Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Clinical Phytoscience
Published in: Clinical Phytoscience 1/2018

Open Access 01-12-2018 | Original contribution

Hepatoprotective, antihyperglycemic and antidiabetic effects of Dendrophthoe pentandra leaf extract in rats

Authors: Mahadi Hasan, Mohammad Tuhin Ali, Rifat Khan, Parag Palit, Aminul Islam, Veronique Seidel, Rabeya Akter, Laizuman Nahar

Published in: Clinical Phytoscience | Issue 1/2018

Login to get access

Abstract

Background

Dendrophthoe pentandra (L.) Miq. is a mistletoe species used in traditional medicine. Juice of leaves is used in wound healing, skin infection and cancer; whereas the whole plant is used to treat hypertension and cough. D. pentandra leaf extract has attracted interest due to its pharmacological properties including antioxidant, cytotoxicity and anti-inflammatory effects. In this study, we have investigated the hepatopotective, antihyperglycemic and antidiabetic potential of D. pentandra leaf extracts in rats.

Methods

D. pentandra leaf methanolic extract (DPLME) at a fixed dose of 400 mg/kg body weight was evaluated for its effects on fasting glucose levels of rats. DPLME at the same dose was also used to determine the antidiabetic potential in alloxan-induced diabetic rats and the hepatopotective effects on Paracetamol (PCM) intoxicated rats.

Results

Oral administration of DPLME exhibited a significantly notable oral glucose tolerance in rats. Single doses of the DPLME displayed very significant antidiabetic activity which was comparable to the activity of the standard antihyperglycemic agent Metformin (MET). DPLME also offered significant hepatoprotection to PCM-intoxicated rats at levels commensurable to the standard hepatoprotective drug Silymarin (SIL).

Conclusions

The results of the present study showed that the DPLME possesses hepatopotective, antihyperglycemic and antidiabetic activity. All these results could be due to the presence of the bioactive components in the extract and this warrant further investigation on the nature of the phytochemical(s) responsible for the observed effects.
Literature
1.
Endharti AT, Permana S. Extract from mango mistletoes Dendrophthoe pentandra ameliorates TNBS-induced colitis by regulating CD4+ T cells in mesenteric lymph nodes. BMC Complement Altern Med. 2017;17(1):468.CrossRefPubMedPubMedCentral
2.
Syazana NA, Zainuddin NASN, Sul’ain MD. Phytochemical analysis, toxicity and cytotoxicity evaluation of Dendropthoe pentandra leaves extracts. Int J Appl Biol Pharm. 2015;6:109–16.
3.
Artanti N, Ma’arifa Y, Hanafi M. Isolation and identification of active antioxidant compound from star fruit (Averrhoa carambola) mistletoe (Dendrophthoe pentandra L.) Miq. Ethanol extract. J Appl Sci. 2006;6:1659–63.CrossRef
4.
Ang HY, Subramani T, Yeap SK, Omar AR, Ho WY, Abdullah MP, et al. Immunomodulatory effects of Potentilla indica and Dendrophthoe pentandra on mice splenocytes and thymocytes. Exp Ther Med. 2014;7(6):1733–7.CrossRefPubMedPubMedCentral
5.
Wild SH, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030: response to Rathman and Giani. Diabetes Care. 2004;27(10):2569.CrossRef
6.
David EJ, Ohio James MF. Evaluation and prevention of diabetic neuropathy. Am Fam Physician. 2005;71(11):2123–8.
7.
Kristova V, Liskova S, Sotnikova R, Vojtko R, Kurtanský A. Sulodexide improves endothelial dysfunction in streptozotocin-induced diabetes in rats. Physiol Res. 2008;57(3):491–4.PubMed
8.
Chaudhury A, Duvoor C, Dendi VSR, Kraleti S, Chada A, Ravilla R, et al. Clinical review of antidiabetic drugs: implications for type 2 diabetes mellitus management. Front Endocrinol. 2017;8:6.CrossRef
9.
Muniyappa R, Lee S, Chen H, Quon MJ. Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage. Am J Physiol Endocrinol Metab. 2008;294(1):E15–26.CrossRefPubMed
10.
11.
Chattopadhyay RR. Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: part II. J Ethnopharmacol. 2003;89(2):217–9.CrossRefPubMed
12.
Rajagopal K, Sasikala K. Antihyperglycaemic and antihyperlipidaemic effects of Nymphaea stellata in alloxan-induced diabetic rats. Singap Med J. 2008;49(2):137–41.
13.
Mukherjee PK, Sahoo AK, Narayanan N, Kumar NS, Ponnusankar S. Lead finding from medicinal plants with hepatoprotective potentials. Expert Opin Drug Discov. 2009;4(5):545–76.CrossRefPubMed
14.
Alberti KGMM, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus. Provisional report of a WHO consultation. Diabet Med. 1998;15(7):539–53.CrossRefPubMed
15.
Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years⊥. J Nat Prod. 2007;70(3):461–77.CrossRefPubMed
16.
Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food Chem Toxicol. 2006;44(2):198–206.CrossRefPubMed
17.
Joy K, Kuttan R. Anti-diabetic activity of Picrorhiza kurroa extract. J Ethnopharmacol. 1999;67(2):143–8.CrossRefPubMed
18.
Hönes J, Müller P, Surridge N. The technology behind glucose meters: test strips. Diabetes Technol Ther. 2008;10(Suppl 1):10–26.CrossRef
19.
Kumar D, Kumar S, Kohli S, Arya R, Gupta J. Antidiabetic activity of methanolic bark extract of Albizia odoratissima Benth. In alloxan induced diabetic albino mice. Asian Pac J Trop Med. 2011;4(11):900–3.CrossRefPubMed
20.
Barham D, Trinder P. An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst. 1972;97(151):142–5.CrossRefPubMed
21.
Achliya GS, Wadodkar SG, Dorle AK. Evaluation of hepatoprotective effect of Amalkadi Ghrita against carbon tetrachloride-induced hepatic damage in rats. J Ethnopharmacol. 2004;90(2–3):229–32.CrossRefPubMed
22.
Gowda S, Desai PB, Hull VV, Math AAK, Vernekar SN, Kulkarni SS. A review on laboratory liver function tests. Pan Afr Med J. 2009;3:17.PubMedPubMedCentral
23.
24.
25.
Eddouks M, Maghrani M, Lemhadri A, Ouahidi M-L, Jouad H. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco (Tafilalet). J Ethnopharmacol. 2002;82(2):97–103.CrossRefPubMed
26.
Klein R. Hyperglycemia and microvascular and macrovascular disease in diabetes. Diabetes Care. 1995;18(2):258–68.CrossRefPubMed
27.
Bolen S, Feldman L, Vassy J, Wilson L, Yeh H-C, Marinopoulos S, et al. Systematic review: comparative effectiveness and safety of oral medications for type 2 diabetes mellitus. Ann Intern Med. 2007;147(6):386–99.CrossRefPubMed
28.
Endharti AT, Wulandari A, Listyana A, Norahmawati E, Permana S. Dendrophthoe pentandra (L.) Miq. Extract effectively inhibits inflammation, proliferation and induces p53 expression on colitis-associated colon cancer. BMC Complement Altern Med. 2016;16(1):374.CrossRefPubMedPubMedCentral
29.
Elsyana V, Bintang M, Priosoeryanto BP. Cytotoxicity and antiproliferative activity assay of clove mistletoe (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Adv Pharmacol Sci. 2016;2016:3242698.PubMedPubMedCentral
30.
Zamani A, Jusoh SAM, Al-Jamal HAN. Sul’ain MD, Johan MF. Anti-proliferative effects of Dendrophthoe pentandra methanol extract on BCR/ABL-positive and imatinib-resistant leukemia cell lines. Asian Pac J Cancer Prev. 2016;17(11):4857–61.PubMedPubMedCentral
31.
Foretz M, Guigas B, Bertrand L, Pollak M, Viollet B. Metformin: from mechanisms of action to therapies. Cell Metab. 2014;20(6):953–66.CrossRefPubMed
32.
Fitrilia T, Bintang M, Safithri M. Phytochemical screening and antioxidant activity of clove mistletoe leaf extract (Dendrophthoe pentandra (L.) Miq.). IOSR J Pharm. 2015;5(8):13–8.
33.
Yee L, Fauzi N, Najihah N, Daud N, Sulain M. Study of Dendrophthoe pentandra ethyl acetate extract as potential anticancer candidate on safety and toxicity aspects. J anal. Pharm Res. 2017;6(1):00167.
34.
Grover J, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol. 2002;81(1):81–100.CrossRefPubMed
35.
Cordero-Herrera I, Martín MA, Bravo L, Goya L, Ramos S. Cocoa flavonoids improve insulin signalling and modulate glucose production via AKT and AMPK in HepG2 cells. Mol Nutr Food Res. 2013;57(6):974–85.CrossRefPubMed
36.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50(6):537–46.PubMed
37.
Zhang X, Liang W, Mao Y, Li H, Yang Y, Tan H. Hepatic glucokinase activity is the primary defect in alloxan-induced diabetes of mice. Biomed Pharmacother. 2009;63(3):180–6.CrossRefPubMed
38.
Grover J, Vats V, Rathi S. Anti-hyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol. 2000;73(3):461–70.CrossRefPubMed
39.
Patel D, Prasad S, Kumar R, Hemalatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed. 2012;2(4):320–30.CrossRefPubMedPubMedCentral
40.
Ekins S, Wrighton SA. The role of CYP2B6 in human xenobiotic metabolism. Drug Metab Rev. 1999;31(3):719–54.CrossRefPubMed
41.
Giannini EG, Testa R, Savarino V. Liver enzyme alteration: a guide for clinicians. Can Med Assoc J. 2005;172(3):367–79.CrossRef
42.
43.
Kumar G, Banu GS, Pappa PV, Sundararajan M, Pandian MR. Hepatoprotective activity of Trianthema portulacastrum L. against paracetamol and thioacetamide intoxication in albino rats. J Ethnopharmacol. 2004;92(1):37–40.CrossRefPubMed
44.
Ahsan MR, Islam KM, Bulbul IJ, Musaddik MA, Haque E. Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride-induced hepatotoxicity in rats. Eur J Sci Res. 2009;37(2):302–10.
45.
Pradhan S, Girish C. Hepatoprotective herbal drug, silymarin from experimental pharmacology to clinical medicine. Indian J Med Res. 2006;124(5):491–504.PubMed
46.
Al-Sayed E, Martiskainen O, Seif el-Din SH, Sabra A-NA, Hammam OA, El-Lakkany NM, et al. Hepatoprotective and antioxidant effect of Bauhinia hookeri extract against carbon tetrachloride-induced hepatotoxicity in mice and characterization of its bioactive compounds by HPLC-PDA-ESI-MS/MS. Biomed Res Int. 2014;2014:245171.CrossRefPubMedPubMedCentral
47.
Nithianantham K, Shyamala M, Chen Y, Latha LY, Jothy SL, Sasidharan S. Hepatoprotective potential of Clitoria ternatea leaf extract against paracetamol induced damage in mice. Molecules. 2011;16(12):10134–45.CrossRefPubMed
48.
Atta A, Elkoly T, Mouneir S, Kamel G, Alwabel N, Zaher S. Hepatoprotective effect of methanol extracts of Zingiber officinale and Cichorium intybus. Indian J Pharm Sci. 2010;72(5):564–70.CrossRefPubMedPubMedCentral
Metadata
Title
Hepatoprotective, antihyperglycemic and antidiabetic effects of Dendrophthoe pentandra leaf extract in rats
Authors
Mahadi Hasan
Mohammad Tuhin Ali
Rifat Khan
Parag Palit
Aminul Islam
Veronique Seidel
Rabeya Akter
Laizuman Nahar
Publication date
01-12-2018
Publisher
Springer Berlin Heidelberg
Published in
Clinical Phytoscience / Issue 1/2018
Electronic ISSN: 2199-1197
DOI
https://doi.org/10.1186/s40816-018-0076-9

Other articles of this Issue 1/2018

Clinical Phytoscience 1/2018 Go to the issue

Original contribution

Results of a randomised controlled study on the efficacy of a combination of saline irrigation and Sinupret syrup phytopreparation in the treatment of acute viral rhinosinusitis in children aged 6 to 11 years

Original contribution

Neuromodulatory effect of solvent fractions of Africa eggplant (Solanium dadyphyllum) against KCN-induced mitochondria damage, viz. NADH-succinate dehydrogenase, NADH- cytochrome c reductase, and succinate-cytochrome c reductase

Original contribution

Pharmacological activities of leaf and bark extracts of a medicinal mangrove plant Avicennia officinalis L.

Original contribution

A tiered approach to investigate the mechanism of anti-inflammatory activity of an herbal medicinal product containing a fixed combination of thyme herb and primula root extracts

Correction

Correction to: Phytochemical studies, antiangiogenic, antiinflammatory and antioxidant activities of Scyphocephalium ochocoa Warb. (Myristicaceae), medicinal plant from Gabon

Review

Evidence-based phytotherapy in allergic rhinitis