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BMC Cancer
Published in: BMC Cancer 1/2024

Open Access 01-12-2024 | Hepatocellular Carcinoma | Research

Ethanol responsive lnc171 promotes migration and invasion of HCC cells via mir-873-5p/ZEB1 axis

Authors: Shiping Huang, Zhouxiang Liao, Xiao He, Zhenyu Song, Xi Fang, Sha Wen, Lichao Yang, Hui Li, Qi Zhang, Wanling Mo, Xiaojing Cheng, Min He, Xuejing Huang

Published in: BMC Cancer | Issue 1/2024

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Abstract

Backgrounds

Long nonconding RNAs (lncRNAs) have been found to be a vital regulatory factor in the development process of human cancer, and could regarded as diagnostic or prognostic biomarkers for human cancers. Here, we aim to confirm the expression and molecular mechanism of RP11-171K16.5 (lnc171) in hepatocellular carcinoma (HCC).

Methods

Screening of differentially expressed lncRNAs by RNA sequencing. Expression level of gene was studied by quantitative real-time PCR (qRT-PCR). The effects of lnc171, mir-873-5p, and ethanol on migration and invasion activity of cells were studied used transwell assay, and luciferase reporter assay was used to confirm the binding site.

Results

RNA sequencing showed that lnc171 was markedly up-regulated in HCC. siRNA-mediated knockdown of lnc171 repressed the migration and invasion ability of HCC cells. Bioinformatic analysis, dual luciferase reporter assay, and qRT-PCR indicated that lnc171 interacted with mir-873-5p in HCC cells, and Zin-finger E-box binding homeobox (ZEB1) was a downstream target gene of mir-873-5p. In addition, lnc171 could enhance migration and invasion ability of HCC cells by up-regulating ZEB1 via sponging mir-873-5p. More interestingly, ethanol stimulation could up-regulate the increase of lnc171, thereby regulating the expression of competing endogenous RNA (ceRNA) network factors which lnc171 participated in HCC cells.

Conclusions

Our date demonstrates that lnc171 was a responsive factor of ethanol, and plays a vital role in development of HCC via binding of mir-873-5p.
Appendix
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Literature
1.
Reina S, Tatsuo K, Osamu Y, et al. Exosomes and Hepatocellular Carcinoma: from bench to Bedside. Int J Mol Sci. 2019;20(6):1406.CrossRef
2.
Torre LA, Bray F, Siegel RL, et al. Global cancer statistics, 2012. CA Cancer J Clin. 2015;65(2):87–108.CrossRefPubMed
3.
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin. 2015;65(1):5–29.
4.
5.
Xu FF, Zha GQ, Wu YP, et al. Overexpressing lncRNA SNHG16 inhibited HCC proliferation and chemoresistance by functionally sponging hsa-miR-93. Onco Targets Ther. 2018;11:8855–63.CrossRefPubMedPubMedCentral
6.
Zhang Y, Luo MY, Cui XH, et al. Long noncoding RNA NEAT1 promotes ferroptosis by modulating the miR-362-3p/MIOX axis as a ceRNA. Cell Death Differ. 2022;29(9):1850–63.CrossRefPubMedPubMedCentral
7.
Deng L, Yang SB, Xu FF, Zhang JH. Long noncoding RNA CCAT1 promotes hepatocellular carcinoma progression by functioning as let-7 sponge. J Exp Clin Cancer Res. 2015;34:18.CrossRefPubMedPubMedCentral
8.
Mary CB, Amanda CD, Antonis K. LNCcation: lncRNA localization and function. J Cell Biol. 2021;220(2):e202009045.CrossRef
9.
10.
Chen TX, Liu RK, Niu YS, et al. HIF-1α-activated long non-coding RNA KDM4A-AS1 promotes hepatocellular carcinoma progression via the miR-411-5p/KPNA2/AKT pathway. Cell Death Dis. 2021;12(12):1152.CrossRefPubMedPubMedCentral
11.
Shan Lu L, Dong X, Jing, et al. Abnormal lncRNA CCAT1/microRNA-155/SIRT1 axis promoted inflammatory response and apoptosis of tubular epithelial cells in LPS caused acute kidney injury. Mitochondrion. 2020;53:76–90.CrossRefPubMed
12.
13.
Xue Wang C, Gao F, Feng, et al. Construction and analysis of competing endogenous RNA networks for breast Cancer based on TCGA dataset. Biomed Res Int. 2020;2020:4078596.PubMedPubMedCentral
14.
Gao YN, Wang ZW, Yang X et al. Aflatoxin M1 and ochratoxin A induce a competitive endogenous RNA regulatory network of intestinal immunosuppression by whole-transcriptome analysis. Sci Total Environ. 2022. 14;158777.
15.
Chao H, Ma H, Sun J, et al. Whole-transcriptome analysis of non-coding RNA alteration in porcine alveolar macrophage exposed to aflatoxin B1. Toxins (Basel). 2022;14(6):373.CrossRefPubMed
16.
Yang JD, Hainaut P, Gregory J, Gores, et al. A global view of hepatocellular carcinoma: trends, risk, prevention and management. Nat Rev Gastroenterol Hepatol. 2019;16(10):589–604.CrossRefPubMedPubMedCentral
17.
Choi MR, Cho S, Kim D-J, et al. Effects of ethanol on expression of coding and noncoding RNAs in murine neuroblastoma Neuro2a cells. Int J Mol Sci. 2022;23(13):7294.CrossRefPubMedPubMedCentral
18.
Wu Y, Qi YB, Bai YQ, et al. LncRNA 1700020I14Rik promotes AKR1B10 expression and activates Erk pathway to induce hepatocyte damage in alcoholic hepatitis. Cell Death Discov. 2022;8(1):374.CrossRefPubMedPubMedCentral
19.
Yan YF, Ren L, Liu Y, et al. Long non-coding RNA CRNDE as potential biomarkers facilitate inflammation and apoptosis in alcoholic liver disease. Aging. 2021;13(19):23233–44.CrossRefPubMedPubMedCentral
20.
Huang XJ, Sun LY, Wen S, et al. RNA sequencing of plasma exosomes revealed novel functional long noncoding RNAs in hepatocellular carcinoma. Cancer Sci. 2020;111(9):3338–49.CrossRefPubMedPubMedCentral
21.
Li YH, Zhong M, Zang HL, Tian XF. The E3 Ligase for Metastasis Associated 1 protein, TRIM25, is targeted by microRNA-873 in Hepatocellular Carcinoma. Exp Cell Res. 2018;368(1):37–41.CrossRefPubMed
22.
Qin G, Tu X, Li H, Cao P, Chen X, Song J, et al. Long noncoding RNA p53-stabilizing and activating RNA promotes p53 signaling by inhibiting heterogeneous nuclear ribonucleoprotein K deSUMOylation and suppresses hepatocellular carcinoma. Hepatology. 2020;71:112–29.CrossRefPubMed
23.
Hu JJ, Zhou C, Luo X, Luo SZ, Li ZH, Xu ZX, et al. Linc-SCRG1 accelerates progression of hepatocellular carcinoma as a ceRNA of miR26a to derepress SKP2. J Exp Clin Cancer Res. 2021;40:26.CrossRefPubMedPubMedCentral
24.
25.
Zhang Y, Zhang C, Zhao Q, et al. The miR-873/NDFIP1 Axis promotes Hepatocellular Carcinoma Growth and Metastasis through the AKT/mTOR-Mediated Warburg Effect. Am J Cancer Res. 2019;9(5):927–44.PubMedPubMedCentral
26.
Wang L, Jiang F, Ma F, et al. Mir-873-5p suppresses cell proliferation and epithelial-mesenchymal transition via directly targeting Jumonji Domain-Containing protein 8 through the NF-kappaB pathway in Colorectal Cancer. J Cell Commun Signal. 2019;13(4):549–60.CrossRefPubMedPubMedCentral
27.
Chen X, Zhang Y, Shi Y, et al. MiR-873 acts as a Novel Sensitizer of Glioma cells to cisplatin by targeting Bcl-2. Int J Oncol. 2015;47(4):1603–11.CrossRefPubMed
28.
Wang Z, Liu W, Wang C, et al. Mir-873-5p inhibits cell migration and invasion of papillary thyroid cancer via regulation of CXCL16. Onco Targets Ther. 2020;13:1037–46.CrossRefPubMedPubMedCentral
29.
Tang L, Chen Y, Tang X, et al. Long noncoding RNA DCST1-AS1 promotes cell proliferation and metastasis in triple-negative breast cancer by forming a positive regulatory loop with mir-873-5p and MYC. J Cancer. 2020;11:311–23.CrossRefPubMedPubMedCentral
30.
Li G, Xu Y, Wang S, et al. Mir-873-5p inhibits cell migration, invasion and epithelial-mesenchymal transition in colorectal cancer via targeting ZEB1. Pathol Res Pract. 2019;215(1):34–9.CrossRefPubMed
31.
Xiufeng H, Wang YMJ et al. LncRNA TDRG1 promotes the metastasis of NSCLC cell through regulating mir-873-5p/ZEB1 axis. J Cell Biochem. 2019; Online ahead of print.
32.
Chen H, Zhu D, Zheng Z, et al. CEP55 promotes epithelial mesenchymal transition in renal cell carcinoma through PI3K/AKT/mTOR pathway. Clin Transl Oncol. 2019;21(7):939–49.CrossRefPubMed
33.
Morillo-bnernal J, Fernandez LP, Santisteban P. FOXE1 regulates migration and invasion in thyroid cancer cells and targets ZEB1. Endocr Relat Cancer. 2020;27(3):137–51.CrossRef
34.
Bray F, Ferlay J, Soerjomataram I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68:394–424.CrossRefPubMed
35.
Bichitra P, Monika L, Jesper BA. Lipid alterations in chronic liver disease and liver cancer. JHEP Rep. 2022;4(6):100479.CrossRef
Metadata
Title
Ethanol responsive lnc171 promotes migration and invasion of HCC cells via mir-873-5p/ZEB1 axis
Authors
Shiping Huang
Zhouxiang Liao
Xiao He
Zhenyu Song
Xi Fang
Sha Wen
Lichao Yang
Hui Li
Qi Zhang
Wanling Mo
Xiaojing Cheng
Min He
Xuejing Huang
Publication date
01-12-2024
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2024
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-024-12309-3

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