Skip to main content
Top
Published in: Lasers in Medical Science 2/2014

01-03-2014 | Original Article

Healing of normal and osteopenic bone with titanium implant and low-level laser therapy (GaAlAs): a histomorphometric study in rats

Authors: Luana Marotta Reis de Vasconcellos, Mary Anne Moreira Barbara, Camila Porto Deco, Juliana Campos Junqueira, Renata Falchete do Prado, Ana Lia Anbinder, Luis Gustavo Oliveira de Vasconcellos, Carlos Alberto Alves Cairo, Yasmin Rodarte Carvalho

Published in: Lasers in Medical Science | Issue 2/2014

Login to get access

Abstract

The study investigates the influence of low-level laser therapy (LLLT) on bone healing in the femur of osteopenic and normal rats with titanium implants. Ovariectomy and control group were randomly submitted to LLLT, which was applied by gallium-aluminum-arsenium (GaAlAs) laser at the surgical site before and after placing the implant, for seven times. Histomorphometric and statistical analysis were performed. Most irradiated groups showed higher values than the nonirradiated groups. The GaAlAs infrared diode laser may improve the osseointegration process in osteopenic and normal bone, particularly based on its effects in the initial phase of bone formation.
Literature
1.
go back to reference Pinheiro ALB, Gerbi MEMM (2006) Photobioengineering of the bone repair process. Photomed Laser Surg 24:169–178PubMedCrossRef Pinheiro ALB, Gerbi MEMM (2006) Photobioengineering of the bone repair process. Photomed Laser Surg 24:169–178PubMedCrossRef
2.
go back to reference Deco C, Marchini A, Barbara MA, Vasconcellos L, Rocha R, Marchini L (2011) Negative effects of alcohol intake and estrogen deficiency combination on osseointegration in a rat model. J Oral Implantol 37:633–639PubMedCrossRef Deco C, Marchini A, Barbara MA, Vasconcellos L, Rocha R, Marchini L (2011) Negative effects of alcohol intake and estrogen deficiency combination on osseointegration in a rat model. J Oral Implantol 37:633–639PubMedCrossRef
3.
go back to reference Stübinger S, Ghanaati S, Saldamli B, Kirkpatrick CJ, Sader R (2009) Er: YAG laser osteotomy: preliminary clinical and histological results of a new technique for contact-free bone surgery. Eur Surg Res 42:150–156PubMedCrossRef Stübinger S, Ghanaati S, Saldamli B, Kirkpatrick CJ, Sader R (2009) Er: YAG laser osteotomy: preliminary clinical and histological results of a new technique for contact-free bone surgery. Eur Surg Res 42:150–156PubMedCrossRef
4.
go back to reference Genant HK, Cooper C, Poor G et al (1999) Interim report and recommendations of the World Health Organization Task Force for osteoporosis. Osteoporos Int 10:259–264PubMedCrossRef Genant HK, Cooper C, Poor G et al (1999) Interim report and recommendations of the World Health Organization Task Force for osteoporosis. Osteoporos Int 10:259–264PubMedCrossRef
5.
go back to reference Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:171–192CrossRef Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:171–192CrossRef
6.
go back to reference Frost HM, Jee WS (1992) On the rat model of human osteopenics and osteoporoses. Bone Miner 18:227–236PubMedCrossRef Frost HM, Jee WS (1992) On the rat model of human osteopenics and osteoporoses. Bone Miner 18:227–236PubMedCrossRef
7.
go back to reference Pires-Oliveira DAA, Oliveira RF, Amadei SU, Pacheco-Soares C, Rocha RF (2010) Laser 904 nm action on bone repair in rats with osteoporosis. Osteoporos Int 21:2109–2114PubMedCrossRef Pires-Oliveira DAA, Oliveira RF, Amadei SU, Pacheco-Soares C, Rocha RF (2010) Laser 904 nm action on bone repair in rats with osteoporosis. Osteoporos Int 21:2109–2114PubMedCrossRef
8.
go back to reference Renno ACM, Moura F, Bossini P, Parizotto N (2006) Effects of 830 nm laser used in two doses, on biomechanical properties of osteopenic rats. Photomed Laser Surg 24:204–208 Renno ACM, Moura F, Bossini P, Parizotto N (2006) Effects of 830 nm laser used in two doses, on biomechanical properties of osteopenic rats. Photomed Laser Surg 24:204–208
9.
go back to reference Gerbi MEMM, Pinheiro ALB, Marzola C et al (2005) Assessment of bone repair associated with the use of organic bovine bone and membrane irradiated at 830 nm. Photomed Laser Surg 23:382–388PubMedCrossRef Gerbi MEMM, Pinheiro ALB, Marzola C et al (2005) Assessment of bone repair associated with the use of organic bovine bone and membrane irradiated at 830 nm. Photomed Laser Surg 23:382–388PubMedCrossRef
10.
go back to reference Gerbi MEMM, Marques AMC, Ramalho LMP et al (2008) Infrared laser light further improves bone healing when associated with bone morphogenic proteins: an in vivo study in a rodent model. Photomed Laser Surg 26:55–60PubMedCrossRef Gerbi MEMM, Marques AMC, Ramalho LMP et al (2008) Infrared laser light further improves bone healing when associated with bone morphogenic proteins: an in vivo study in a rodent model. Photomed Laser Surg 26:55–60PubMedCrossRef
11.
go back to reference Lopes CB, Pinheiro ALB, Sathaiah S, Silva NS, Salgado MAC (2007) Infrared laser photobiomodulation (830 nm) on bone tissue around dental implants: a raman spectroscopy and scanning electronic microscopy study in rabbits. Photomed Laser Surg 25:96–101PubMedCrossRef Lopes CB, Pinheiro ALB, Sathaiah S, Silva NS, Salgado MAC (2007) Infrared laser photobiomodulation (830 nm) on bone tissue around dental implants: a raman spectroscopy and scanning electronic microscopy study in rabbits. Photomed Laser Surg 25:96–101PubMedCrossRef
12.
go back to reference Diniz JS, Nicolau RA, de Melo Ocarino N, do Carmo Magalhães F, de Oliveira Pereira RD, Serakides R (2009) Effect of low-power gallium-aluminum-arsenium laser therapy (830 nm) in combination with bisphosphonate treatment on osteopenic bone structure: an experimental animal study. Lasers Med Sci 24:347–352PubMedCrossRef Diniz JS, Nicolau RA, de Melo Ocarino N, do Carmo Magalhães F, de Oliveira Pereira RD, Serakides R (2009) Effect of low-power gallium-aluminum-arsenium laser therapy (830 nm) in combination with bisphosphonate treatment on osteopenic bone structure: an experimental animal study. Lasers Med Sci 24:347–352PubMedCrossRef
13.
go back to reference Obradovic RR, Kesic LG, Pesevska S (2009) Influence of low-level laser therapy on biomaterial osseointegration: a mini review. Lasers Med Sci 24:447–451PubMedCrossRef Obradovic RR, Kesic LG, Pesevska S (2009) Influence of low-level laser therapy on biomaterial osseointegration: a mini review. Lasers Med Sci 24:447–451PubMedCrossRef
14.
go back to reference Favaro-Pipi E, Feitosa SM, Ribeiro DA, Bossini P, Oliveira P, Parizotto NA, Renno AC (2010) Comparative study of the effects of low-level therapy on bone defects in tibias of rats. Lasers Med Sci 25:727–732PubMedCrossRef Favaro-Pipi E, Feitosa SM, Ribeiro DA, Bossini P, Oliveira P, Parizotto NA, Renno AC (2010) Comparative study of the effects of low-level therapy on bone defects in tibias of rats. Lasers Med Sci 25:727–732PubMedCrossRef
15.
go back to reference Bossini PS, Rennó AC, Ribeiro DA et al (2012) Low level laser therapy (830 nm) improves bone repair in osteoporotic rats: similar outcomes at two different dosages. Exp Gerontol 47:136–142PubMedCrossRef Bossini PS, Rennó AC, Ribeiro DA et al (2012) Low level laser therapy (830 nm) improves bone repair in osteoporotic rats: similar outcomes at two different dosages. Exp Gerontol 47:136–142PubMedCrossRef
16.
go back to reference Nogueira GT, Mesquita-Ferrari RA, Souza NH et al (2012) Effect of low-level laser therapy on proliferation, differentiation, and adhesion of steroid-treated osteoblasts. Lasers Med Sci 27:1189–1193PubMedCrossRef Nogueira GT, Mesquita-Ferrari RA, Souza NH et al (2012) Effect of low-level laser therapy on proliferation, differentiation, and adhesion of steroid-treated osteoblasts. Lasers Med Sci 27:1189–1193PubMedCrossRef
17.
go back to reference Nissan J, Assif D, Gross MD, Yaffe A, Binderman I (2006) Effect of low intensity laser irradiation on surgically created bony defects in rats. J Oral Rehabil 33:619–624PubMedCrossRef Nissan J, Assif D, Gross MD, Yaffe A, Binderman I (2006) Effect of low intensity laser irradiation on surgically created bony defects in rats. J Oral Rehabil 33:619–624PubMedCrossRef
18.
go back to reference Doshi-Mehta G, Bhad-Patil WA (2012) Efficacy of low-intensity laser therapy in reducing treatment time and orthodontic pain: a clinical investigation. Am J Orthod Dentofac Orthop 141:289–297CrossRef Doshi-Mehta G, Bhad-Patil WA (2012) Efficacy of low-intensity laser therapy in reducing treatment time and orthodontic pain: a clinical investigation. Am J Orthod Dentofac Orthop 141:289–297CrossRef
19.
go back to reference Pretel H, Lizarelli RFZ, Ramalho LTO (2007) Effect of low-level laser therapy on bone repair: histological study in rats. Lasers Surg Med 39:788–796PubMedCrossRef Pretel H, Lizarelli RFZ, Ramalho LTO (2007) Effect of low-level laser therapy on bone repair: histological study in rats. Lasers Surg Med 39:788–796PubMedCrossRef
20.
go back to reference Pinheiro ALB, Limeira FA Jr, Gerbi MEMM, Ramalho LMP, Marzola C, Ponzi EAC (2003) Effect of low-level therapy on the repair of bone defects grafted with inorganic bovine bone. Braz Dent J 14:177–181PubMedCrossRef Pinheiro ALB, Limeira FA Jr, Gerbi MEMM, Ramalho LMP, Marzola C, Ponzi EAC (2003) Effect of low-level therapy on the repair of bone defects grafted with inorganic bovine bone. Braz Dent J 14:177–181PubMedCrossRef
21.
go back to reference Ré Poppi R, Da Silva AL, Nacer RS et al (2011) Evaluation of the osteogenic effect of low-level laser therapy (808 nm and 660 nm) on bone defects induced in the femurs of female rats submitted to ovariectomy. Lasers Med Sci 26:515–522PubMedCrossRef Ré Poppi R, Da Silva AL, Nacer RS et al (2011) Evaluation of the osteogenic effect of low-level laser therapy (808 nm and 660 nm) on bone defects induced in the femurs of female rats submitted to ovariectomy. Lasers Med Sci 26:515–522PubMedCrossRef
22.
go back to reference Pinheiro ALB, Oliveira MG, Martins PPM, Ramalho LMP, Oliveira MAM, Junior NA, Nicolau RA (2001) Biomodulatory effects of low-level laser therapy on bone regeneration. Laser Ther 13:73–79CrossRef Pinheiro ALB, Oliveira MG, Martins PPM, Ramalho LMP, Oliveira MAM, Junior NA, Nicolau RA (2001) Biomodulatory effects of low-level laser therapy on bone regeneration. Laser Ther 13:73–79CrossRef
23.
go back to reference Dörtbudak O, Haas R, Mailath-Pokorny G (2002) Effect of low-power laser irradiation on bony implant sites. Clin Oral Implants Res 13:288–292PubMedCrossRef Dörtbudak O, Haas R, Mailath-Pokorny G (2002) Effect of low-power laser irradiation on bony implant sites. Clin Oral Implants Res 13:288–292PubMedCrossRef
24.
go back to reference Merli LAS, Santos MT, Genovese WJ, Faloppa F (2005) Effects of low-intensity laser irradiation on process of bone repair. Photomed Laser Surg 2:212–215CrossRef Merli LAS, Santos MT, Genovese WJ, Faloppa F (2005) Effects of low-intensity laser irradiation on process of bone repair. Photomed Laser Surg 2:212–215CrossRef
25.
go back to reference Duarte PM, César Neto JB, Gonçalves PF, Sallum EA, Nociti FH (2003) Estrogen deficiency affects bone healing around titanium implants: a histometric study in rats. Implant Dent 12:340–346PubMedCrossRef Duarte PM, César Neto JB, Gonçalves PF, Sallum EA, Nociti FH (2003) Estrogen deficiency affects bone healing around titanium implants: a histometric study in rats. Implant Dent 12:340–346PubMedCrossRef
26.
go back to reference Prado RF, Silveira VAS, Rocha RF, Vasconcellos LMR, Carvalho YR (2012) Effects of experimental osteoporosis and low calcium intake on post-extraction sockets of rats. Int J Exp Pathol 93:139–147PubMedCrossRef Prado RF, Silveira VAS, Rocha RF, Vasconcellos LMR, Carvalho YR (2012) Effects of experimental osteoporosis and low calcium intake on post-extraction sockets of rats. Int J Exp Pathol 93:139–147PubMedCrossRef
27.
go back to reference Khadra M (2005) The effect of low level laser irradiation on implant-tissue interaction. In vivo and in vitro studies. Swed Dent J 172:1–63 Khadra M (2005) The effect of low level laser irradiation on implant-tissue interaction. In vivo and in vitro studies. Swed Dent J 172:1–63
28.
go back to reference Omasa S, Motoyoshi M, Arai Y, Ejima K, Shimizu N (2012) Low-level laser therapy enhances the stability of orthodontic mini-implants via bone formation related to bmp-2 expression in a rat model. Photomed Laser Surg 30:255–261PubMedCrossRef Omasa S, Motoyoshi M, Arai Y, Ejima K, Shimizu N (2012) Low-level laser therapy enhances the stability of orthodontic mini-implants via bone formation related to bmp-2 expression in a rat model. Photomed Laser Surg 30:255–261PubMedCrossRef
30.
go back to reference Renno ACM, McDonnell PA, Parizotto NA, Laakso EL (2007) The effects of laser irradiation on the osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25:275–280PubMedCrossRef Renno ACM, McDonnell PA, Parizotto NA, Laakso EL (2007) The effects of laser irradiation on the osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25:275–280PubMedCrossRef
31.
go back to reference Ng GY, Fung DT, Leung MC, Guo X (2004) Ultrastructural comparison of medial collateral ligament repair after single or multiple applications of GaAlAs laser in rats. Lasers Surg Med 35:317–323PubMedCrossRef Ng GY, Fung DT, Leung MC, Guo X (2004) Ultrastructural comparison of medial collateral ligament repair after single or multiple applications of GaAlAs laser in rats. Lasers Surg Med 35:317–323PubMedCrossRef
32.
go back to reference Reis FA, Belchior AC, de Carvalho PT et al (2009) Effect of laser therapy (660 nm) on recovery of the sciatic nerve in rats after injury through neurotmesis followed by epineural anastomosis. Lasers Med Sci 24:741–747PubMedCrossRef Reis FA, Belchior AC, de Carvalho PT et al (2009) Effect of laser therapy (660 nm) on recovery of the sciatic nerve in rats after injury through neurotmesis followed by epineural anastomosis. Lasers Med Sci 24:741–747PubMedCrossRef
33.
go back to reference Garavello-Freitas I, Baranauskas V, Joazeiro P, Padovani CR, Dal Pai-Silva M, Cruz-Hofling MA (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochemical Photobiol 70:81–89CrossRef Garavello-Freitas I, Baranauskas V, Joazeiro P, Padovani CR, Dal Pai-Silva M, Cruz-Hofling MA (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochemical Photobiol 70:81–89CrossRef
34.
go back to reference Sasaki Y, Wang S, Ogata Y (2011) Transcriptional regulation of bone sialoprotein gene by CO (2) laser irradiation. J Oral Sci 53:51–59PubMedCrossRef Sasaki Y, Wang S, Ogata Y (2011) Transcriptional regulation of bone sialoprotein gene by CO (2) laser irradiation. J Oral Sci 53:51–59PubMedCrossRef
35.
go back to reference Grassi FR, Ciccolella F, D’Apolito G et al (2011) Effect of low-level laser irradiation on osteoblast proliferation and bone formation. J Biol Regul Homeost Agents 25:603–601PubMed Grassi FR, Ciccolella F, D’Apolito G et al (2011) Effect of low-level laser irradiation on osteoblast proliferation and bone formation. J Biol Regul Homeost Agents 25:603–601PubMed
36.
go back to reference Serakides R, Nunes VA, Ocarino NM, Nascimento EF (2004) Bone effects of the association hyperthyroidism-castration of adult female rats. Arq Bras Endocrinol Metabol 48:875–883PubMedCrossRef Serakides R, Nunes VA, Ocarino NM, Nascimento EF (2004) Bone effects of the association hyperthyroidism-castration of adult female rats. Arq Bras Endocrinol Metabol 48:875–883PubMedCrossRef
37.
go back to reference Saad A, El Yamany M, Abbas O, Yehia M (2010) Possible role of low level laser therapy on bone turnover in ovariectomized rats. Endocr Regul 44:155–163PubMedCrossRef Saad A, El Yamany M, Abbas O, Yehia M (2010) Possible role of low level laser therapy on bone turnover in ovariectomized rats. Endocr Regul 44:155–163PubMedCrossRef
38.
go back to reference Garavello I, Baranauskas V, Cruz-Hofling MA (2004) The effects of low laser irradiation on angiogenesis in injured rat tibiae. Histol Histopathol 19:43–48PubMed Garavello I, Baranauskas V, Cruz-Hofling MA (2004) The effects of low laser irradiation on angiogenesis in injured rat tibiae. Histol Histopathol 19:43–48PubMed
39.
go back to reference Lill CA, Hesseln J, Schlegel U, Eckhardt C, Goldhahn J, Schneider E (2003) Biomechanical evaluation of healing in a non-critical defect in a large animal model of osteoporosis. J Orthop Res 21:836–842PubMedCrossRef Lill CA, Hesseln J, Schlegel U, Eckhardt C, Goldhahn J, Schneider E (2003) Biomechanical evaluation of healing in a non-critical defect in a large animal model of osteoporosis. J Orthop Res 21:836–842PubMedCrossRef
Metadata
Title
Healing of normal and osteopenic bone with titanium implant and low-level laser therapy (GaAlAs): a histomorphometric study in rats
Authors
Luana Marotta Reis de Vasconcellos
Mary Anne Moreira Barbara
Camila Porto Deco
Juliana Campos Junqueira
Renata Falchete do Prado
Ana Lia Anbinder
Luis Gustavo Oliveira de Vasconcellos
Carlos Alberto Alves Cairo
Yasmin Rodarte Carvalho
Publication date
01-03-2014
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 2/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-013-1326-1

Other articles of this Issue 2/2014

Lasers in Medical Science 2/2014 Go to the issue