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BMC Complementary Medicine and Therapies

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Open Access 01-12-2016 | Research article

Gyeji-tang water extract exerts anti-inflammatory activity through inhibition of ERK and NF-κB pathways in lipopolysaccharide-stimulated RAW 264.7 cells

Authors: Sae-Rom Yoo, Yeji Kim, Mee-Young Lee, Ohn-Soon Kim, Chang-Seob Seo, Hyeun-Kyoo Shin, Soo-Jin Jeong

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Abstract

Background

Gyeji-tang (GJT, Guizhi Tang in Chinese, Keishi-to in Japanese) is a traditional herbal decoction composed of 5 medicinal herbs. GJT has been used to treat the common cold, headaches, and fever in Asian countries including Korea, China, and Japan. In the present study, we investigated the inhibitory effect of a water extract of GJT on inflammatory response using the murine macrophage cell line, RAW 264.7.

Methods

RAW 264.7 macrophages were treated with lipopolysaccharide (LPS) to upregulate inflammatory genes. Cells were pretreated with various concentrations of GJT for 4 h and stimulated with LPS for an additional 20 h. Productions of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), and prostaglandin E₂ (PGE₂) were measured by enzyme-linked immunosorbent assays (ELISAs). Protein expressions of heme oxygenase (HO)-1, extracellular signal-regulated kinase (ERK), and nuclear factor kappa-B (NF-κB) were analyzed by immunoblotting.

Results

Treatment with the GJT extract enhanced expression of HO-1 in macrophages without cytotoxicity. GJT extract significantly inhibited proinflammatory cytokines TNF-α and IL-6 in LPS-stimulated cells. GJT suppressed LPS-induced COX-2 expression, leading to a decrease in COX-2-derived PGE₂ level. In addition, GJT extract prevented phosphorylation of ERK and NF-κB translocalization to the nucleus in LPS-treated RAW 264.7 cells.

Conclusion

These data suggest that GJT has anti-inflammatory possibly through blocking ERK and NF-κB signaling pathways.

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Title: Gyeji-tang water extract exerts anti-inflammatory activity through inhibition of ERK and NF-κB pathways in lipopolysaccharide-stimulated RAW 264.7 cells
Authors: Sae-Rom Yoo
Yeji Kim
Mee-Young Lee
Ohn-Soon Kim
Chang-Seob Seo
Hyeun-Kyoo Shin
Soo-Jin Jeong
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-016-1366-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Gyeji-tang water extract exerts anti-inflammatory activity through inhibition of ERK and NF-κB pathways in lipopolysaccharide-stimulated RAW 264.7 cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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