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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Guided Tissue Regeneration | Research

Adjunctive effect of collagen membrane coverage to L-PRF in the treatment of periodontal intrabony defects: a randomized controlled clinical trial with biochemical assessment

Authors: Ramy Mubarak, Doaa Adel-Khattab, Khaled A. Abdel-Ghaffar, Ahmed Youssef Gamal

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Background

The innovation of leukocyte platelet-rich fibrin (L-PRF) has added enormous impact on wound healing dynamics especially the field of periodontal regeneration. The release of growth factors (GF) is thought to improve the clinical outcomes in infrabony defects. The aim of this study was to evaluate the clinical effect of covering L-PRF contained infrabony defects with collagen membranes (CM), and to compare their GF release profile to uncovered L-PRF defects and open flap debridement (OFD).

Methods

Thirty non- smoking patients with infrabony pockets participated to be randomly assigned to OFD group (n = 10), L-PRF group (n = 10), or L-PRF protected CM group (n = 10). Plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL) and the radiographic defect base fill (DBF) were measured at baseline and at 6 month following surgical intervention. Gingival crevicular fluid samples were obtained on days 1, 3, 5, 7, 14, 21 and 30 days following surgery for the Platelet Derived Growth Factor-BB (PDGF-BB) and Vascular Endothelial Growth Factors (VEGF) release profile evaluation.

Results

For all patients, a statistically significant (P ≤ 0.05) reduction in PI, GI, PD and CAL were reported throughout the study period. Differences between the three treatment modalities were not statistically significant. PRF + CM showed a statistically significant DBF compared to OFD and L-PRF groups at follow up. Quantitative analysis of PDGF-BB and VEGF levels demonstrated a statistically significant (P < 0.001) decline between measurement intervals for all groups with no statistically significant differences between the three groups.

Conclusion

Within the limitations of this study, L-PRF coverage with CM may augment defect base fill through its mechanical protective effect without enhancement in the release profile of VEGF and PDGF. The non-significant intergroup differences question the validity of the claimed extra physiologic concentration of GF offered by L-PRF harvests.

Trial registration

The present study was registered at ClinicalTrials.gov (NCT05496608), (11/08/2022).
Appendix
Available only for authorised users
Footnotes
2
Hu-Friedy Mfg. Co., LLC, USA.
 
3
Artinibsa 2% Inibsa Dental S.L.U, Ctra. Sabadell a Granollers, KM 14,5 (C-155), 08185 Lliçà de Vall (Barcelona), Spain.
 
4
Silicon-coated interior, Intraspin®; IntraLockTM, Boca Raton, FL, USA.
 
5
Intraspin®; IntraLockTM, Boca Raton, FL, USA.
 
6
Pearson Dental, Sylmar, Ca
 
7
Tutopatch RTI Surgical Inc, FL, USA.
 
8
Brufen 400 mg; Kahira Pharm. & Chem. Ind. Co., Under license from: Abbott Laboratories.
 
9
Floxamo 500 mg; Amoun Pharmaceutical Company S.A.E. Cairo – Egypt.
 
10
Hexitol; Kahera Pharmaceutical, Cairo, Egypt.
 
11
New IDA, Eagle, Brazil.
 
12
Preva Dental X-ray System, Progeny, Midmark, Illinois, USA.
 
13
DabiAtlante, Sao Paulo, Brazil.
 
14
Oraflow Inc., New York, United States.
 
15
RayBiotech, Norcross, GA.
 
16
Microsoft Excel 2016®, DC, USA.
 
17
IBM SPSS Statistics for Windows, version 20.0. Armonk, NY: IBM Corp, USA.
 
18
Graphpad Software, Boston, USA.
 
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Metadata
Title
Adjunctive effect of collagen membrane coverage to L-PRF in the treatment of periodontal intrabony defects: a randomized controlled clinical trial with biochemical assessment
Authors
Ramy Mubarak
Doaa Adel-Khattab
Khaled A. Abdel-Ghaffar
Ahmed Youssef Gamal
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03332-0

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