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Inflammation Research

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01-06-2020 | Gout | Original Research Paper

Characteristics of voltage-gated potassium currents in monosodium urate induced gouty arthritis in mice

Authors: Jiangru Hong, Jie Qiu, Xiniao Wang, Guangqin Zhang

Published in: Inflammation Research | Issue 6/2020

Abstract

Objective

To evaluate the role of K⁺ channels in pain following gouty arthritis.

Methods

The model of acute gouty arthritis was induced by monosodium urate (MSU) in mice. The swelling degree was determined by measuring the circumference of the ankle joint. Mechanical hyperalgesia was detected by von Frey filaments. Two types of K⁺ currents, A-type currents (I_A) and delayed rectifier currents (I_K), were recorded in dorsal root ganglion (DRG) neurons using patch-clamp techniques.

Results

The swelling degree reached its maximum at 10 h and the minimum pain threshold was maintained between 8 and 48 h after MSU treatment in mice. The amplitudes of I_A and I_K in DRG neurons were moderately increased on day 1 after MSU treatment, and then, they were gradually decreased with times and reached their minimums on day 4 (for I_A) or 5 (for I_K). Compared with control group, the activation curve of I_A was significantly shifted to more positive potential and the recovery time of I_A from inactivation was markedly prolonged, but inactivation and frequency dependence of I_A appeared unaffected in MSU-treated group. Additionally, no change was observed in the activation curve of I_K after MSU treatment. The excitability was significantly higher in the MSU group than in the control group.

Conclusions

MSU-induced gout pain may be related to the hyperexcitability of DRG neurons elicited by decreasing K⁺ currents.

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Title: Characteristics of voltage-gated potassium currents in monosodium urate induced gouty arthritis in mice
Authors: Jiangru Hong
Jie Qiu
Xiniao Wang
Guangqin Zhang
Publication date: 01-06-2020
Publisher: Springer International Publishing
Keywords: Gout
Arthritis
Published in: Inflammation Research / Issue 6/2020
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-020-01343-z

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