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Open Access 01-12-2015 | Short report

Glycoside hydrolase family 32 is present in Bacillus subtilis phages

Authors: Halim Maaroufi, Roger C. Levesque

Published in: Virology Journal | Issue 1/2015

Abstract

Background

Glycoside hydrolase family 32 (GH32) enzymes cleave the glycosidic bond between two monosaccharides or between a carbohydrate and an aglycone moiety. GH32 enzymes have been studied in prokaryotes and in eukaryotes but not in viruses.

Findings

This is the first analysis of GH32 enzymes in Bacillus subtilis phage SP10, ϕNIT1 and SPG24. Phylogenetic analysis, molecular docking and secretability predictions suggest that phage GH32 enzymes function as levan (fructose homopolysaccharide) fructotransferase.

Conclusions

We showed that viruses also contain GH32 enzymes and that our analyses in silico strongly suggest that these enzymes function as levan fructotransferase.

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Title: Glycoside hydrolase family 32 is present in Bacillus subtilis phages
Authors: Halim Maaroufi
Roger C. Levesque
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2015
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-015-0373-6

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