Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
BMC Oral Health
Published in: BMC Oral Health 1/2021

Open Access 01-12-2021 | Gingiva Index | Research article

Early soft tissue response to zirconium oxide and titanium healing abutments in vivo: a study in dogs

Authors: Min Wang, Shuang Zhang, Longjie Chen, Haixiao Zou, Yining Wang, Haibin Xia

Published in: BMC Oral Health | Issue 1/2021

Login to get access

Abstract

Background

This study aimed to investigate the clinical characteristics and early soft tissue response to zirconium oxide (Zr) and titanium (Ti) healing abutments in dogs.

Methods

Eight implants (four at each hemi-mandible) were inserted after bilateral mandibular third and fourth premolars and first molar extraction in dogs. Then, two Zr and two Ti healing abutments were connected to each unilateral mandible eight weeks later. The ligation method was used to create a peri-implant mucositis model and the 24 abutments were divided into four groups: Zr or Ti healing abutments with ligation (ZrL, TiL) or non-ligation (ZrN, TiN). The clinical indices, peri-implant crevicular fluid (PICF), and inflammatory cytokines (TNF-α and IL-1β) were measured and analyzed on days 0 and 28. The dogs were then sacrificed on day 28, soft tissues around the implants were harvested, and inflammation infiltration was tested by immunohistochemistry. Normal distribution test and two-way analysis of variance was used to analyze the data.

Results

The results showed that the clinical indices were similar for Zr and Ti healing abutments. There was significantly more PICF in the ZrL and TiL groups compared to in the ZrN and TiN groups. The TNF-α levels in PICF were significantly different between ZrL and ZrN groups on day 28. And the TNF-α levels in PICF were significantly higher in TiL group on day 28 than that on day 0. However, the number of inflammatory cells was not significantly different between the groups as measured by immunohistochemistry.

Conclusions

These data indicate that soft tissue responses to Zr healing abutments with peri-implant mucositis were comparable to those of Ti healing abutments in vivo, providing a theoretical foundation for the clinical application of Zr abutments.
Literature
1.
Welander M, Abrahamsson I, Berglundh T. The mucosal barrier at implant abutments of different materials. Clin Oral Implants Res. 2008;19(7):635–41.PubMed
2.
Abrahamsson I, Berglundh T, Lindhe J. The mucosal barrier following abutment dis/reconnection. An experimental study in dogs. J Clin Periodontol. 1997;4(8):568–72.CrossRef
3.
Lindhe J, Berglundh T. The interface between the mucosa and the implant. Periodontol. 2000;1998(17):47–54.
4.
Barboza EP, Caula AL, Carvalho WR. Crestal bone loss around submerged and exposed unloaded dental implants: a radiographic and microbiological descriptive study. Implant Dent. 2002;11(2):162–9.CrossRef
5.
Misch CE, Dietsh-Misch F, Hoar J, Beck G, Hazen R, Misch CM. A bone quality-based implant system: first year of prosthetic loading. J Oral Implantol. 1999;25(3):185–97.CrossRef
6.
Linkevicius T, Apse P. Influence of abutment material on stability of peri-implant tissues: a systematic review. Int J Oral Maxillofac Implants. 2008;23(3):449–56.PubMed
7.
Lekholm U, Gunne J, Henry P, Higuchi K, Linden U, Bergstrom C, et al. Survival of the Branemark implant in partially edentulous jaws: a 10-year prospective multicenter study. Int J Oral Maxillofac Implants. 1999;14(5):639–45.PubMed
8.
Ekelund JA, Lindquist LW, Carlsson GE, Jemt T. Implant treatment in the edentulous mandible: a prospective study on Branemark system implants over more than 20 years. Int J Prosthodont. 2003;16(6):602–8.PubMed
9.
Taira M, Kagiya T, Harada H, Sasaki M, Kimura S, Narushima T, et al. Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles. J Mater Sci Mater Med. 2010;21(1):267–75.CrossRef
10.
Siddiqi A, Payne A, De Silva R, Duncan W. Titanium allergy: could it affect dental implant integration? Clin Oral Implants Res. 2011;22(7):673–80.CrossRef
11.
Prestipino V, Ingber A. Esthetic high-strength implant abutments. Part II. J Esthet Dent. 1993;5(2):63–8.CrossRef
12.
Prestipino V, Ingber A. Esthetic high-strength implant abutments. Part I. J Esthet Dent. 1993;5(1):29–36.CrossRef
13.
Holst S, Blatz MB, Hegenbarth E, Wichmann M, Eitner S. Prosthodontic considerations for predictable single-implant esthetics in the anterior maxilla. J Oral Maxillofac Surg. 2005;63(9 Suppl 2):89–96.CrossRef
14.
Denry I, Kelly JR. State of the art of zirconia for dental applications. Dent Mater. 2008;24(3):299–307.CrossRef
15.
van Brakel R, Cune MS, van Winkelhoff AJ, de Putter C, Verhoeven JW, van der Reijden W. Early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man. Clin Oral Implants Res. 2011;22(6):571–7.CrossRef
16.
Scarano A, Piattelli M, Caputi S, Favero GA, Piattelli A. Bacterial adhesion on commercially pure titanium and zirconium oxide disks: an in vivo human study. J Periodontol. 2004;75(2):292–6.CrossRef
17.
Rimondini L, Cerroni L, Carrassi A, Torricelli P. Bacterial colonization of zirconia ceramic surfaces: an in vitro and in vivo study. Int J Oral Maxillofac Implants. 2002;17(6):793–8.PubMed
18.
Degidi M, Artese L, Scarano A, Perrotti V, Gehrke P, Piattelli A. Inflammatory infiltrate, microvessel density, nitric oxide synthase expression, vascular endothelial growth factor expression, and proliferative activity in peri-implant soft tissues around titanium and zirconium oxide healing caps. J Periodontol. 2006;77(1):73–80.CrossRef
19.
Tete S, Mastrangelo F, Bianchi A, Zizzari V, Scarano A. Collagen fiber orientation around machined titanium and zirconia dental implant necks: an animal study. Int J Oral Maxillofac Implants. 2009;24(1):52–8.PubMed
20.
van Brakel R, Meijer GJ, Verhoeven JW, Jansen J, de Putter C, Cune MS. Soft tissue response to zirconia and titanium implant abutments: an in vivo within-subject comparison. J Clin Periodontol. 2012;39(10):995–1001.CrossRef
21.
Barwacz CA, Brogden KA, Stanford CM, Dawson DV, Recker EN, Blanchette D. Comparison of pro-inflammatory cytokines and bone metabolism mediators around titanium and zirconia dental implant abutments following a minimum of 6 months of clinical function. Clin Oral Implants Res. 2015;26(4):e35–41.CrossRef
22.
Zhou Y, Jiang T, Qian M, Zhang X, Wang J, Shi B, et al. Roles of bone scintigraphy and resonance frequency analysis in evaluating osseointegration of endosseous implant. Biomaterials. 2008;29(4):461–74.CrossRef
23.
Orsini E, Salgarello S, Bubalo M, Lazic Z, Trire A, Martini D, et al. Histomorphometric evaluation of implant design as a key factor in peri-implant bone response: a preliminary study in a dog model. Minerva Stomatol. 2009;58(6):263–75.PubMed
24.
Shibli JA, Martins MC, Lotufo RF, Marcantonio E. Microbiologic and radiographic analysis of ligature-induced peri-implantitis with different dental implant surfaces. Int J Oral Maxillofac Implants. 2003;18(3):383–90.PubMed
25.
Lang NP, Bragger U, Walther D, Beamer B, Kornman KS. Ligature-induced peri-implant infection in cynomolgus monkeys. I. Clinical and radiographic findings. Clin Oral Implants Res. 1993;4(1):2–11.CrossRef
26.
Loe H, Theilade E, Jensen SB. Experimental gingivitis in man. J Periodontol. 1965;36:177–87.CrossRef
27.
Loe H. The Gingival Index, the Plaque Index and the Retention Index Systems. J Periodontol. 1967;38(6):610–6.CrossRef
28.
Griffiths GS, Curtis MA, Wilton JM. Selection of a filter paper with optimum properties for the collection of gingival crevicular fluid. J Periodontal Res. 1988;23(1):33–8.CrossRef
29.
Jung RE, Holderegger C, Sailer I, Khraisat A, Suter A, Hammerle CH. The effect of all-ceramic and porcelain-fused-to-metal restorations on marginal peri-implant soft tissue color: a randomized controlled clinical trial. Int J Periodontics Restor Dent. 2008;28(4):357–65.
30.
Albouy JP, Abrahamsson I, Persson LG, Berglundh T. Spontaneous progression of peri-implantitis at different types of implants. An experimental study in dogs. I: clinical and radiographic observations. Clin Oral Implants Res. 2008;19(10):997–1002.CrossRef
31.
Schierano G, Pejrone G, Brusco P, Trombetta A, Martinasso G, Preti G, et al. TNF-alpha TGF-beta2 and IL-1beta levels in gingival and peri-implant crevicular fluid before and after de novo plaque accumulation. J Clin Periodontol. 2008;35(6):532–8.CrossRef
32.
Petkovic AB, Matic SM, Stamatovic NV, Vojvodic DV, Todorovic TM, Lazic ZR, et al. Proinflammatory cytokines (IL-1beta and TNF-alpha) and chemokines (IL-8 and MIP-1alpha) as markers of peri-implant tissue condition. Int J Oral Maxillofac Surg. 2010;39(5):478–85.CrossRef
33.
Panagakos FS, Aboyoussef H, Dondero R, Jandinski JJ. Detection and measurement of inflammatory cytokines in implant crevicular fluid: a pilot study. Int J Oral Maxillofac Implants. 1996;11(6):794–9.PubMed
34.
Ataoglu H, Alptekin NO, Haliloglu S, Gursel M, Ataoglu T, Serpek B, et al. Interleukin-1beta, tumor necrosis factor-alpha levels and neutrophil elastase activity in peri-implant crevicular fluid. Clin Oral Implants Res. 2002;13(5):470–6.CrossRef
35.
Blanco J, Caneiro L, Linares A, Batalla P, Munoz F, Ramos I. Peri-implant soft tissue analyses comparing Ti and ZrO2 abutments: an animal study on beagle dogs. Clin Oral Implants Res. 2016;27(10):1221–6.CrossRef
36.
Sampatanukul T, Serichetaphongse P, Pimkhaokham A. Histological evaluations and inflammatory responses of different dental implant abutment materials: a human histology pilot study. Clin Implant Dent Relat Res. 2018;20(2):160–9.CrossRef
37.
Cury PR, Furuse C, Rodrigues AE, Barbuto JA, Araujo VC, Araujo NS. Interstitial and Langerhans’ dendritic cells in chronic periodontitis and gingivitis. Braz Oral Res. 2008;22(3):258–63.CrossRef
Metadata
Title
Early soft tissue response to zirconium oxide and titanium healing abutments in vivo: a study in dogs
Authors
Min Wang
Shuang Zhang
Longjie Chen
Haixiao Zou
Yining Wang
Haibin Xia
Publication date
01-12-2021
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2021
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-021-01748-0

Other articles of this Issue 1/2021

BMC Oral Health 1/2021 Go to the issue

Study protocol

CariesCare International adapted for the pandemic in children: Caries OUT multicentre single-group interventional study protocol

Research

Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats

Research article

Comparison of two minimally invasive restorative techniques in improving the oral health-related quality of life of pregnant women: a six months randomized controlled trial

Research

Assessing the completeness of periodontal disease documentation in the EHR: a first step in measuring the quality of care

Research article

Analysing the effectiveness of topical bleeding care following tooth extraction in patients receiving dual antiplatelet therapy-retrospective observational study

Research

Farsi version of the CLEFT-Q: translation, cultural adaptation process and reliability