Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 3/2013

01-03-2013 | Original Article

Genotypic characterization of dengue virus strains circulating during 2007-2009 in New Delhi

Authors: Anita Chakravarti, Mayank Singh Chauhan, Suman Kumar, Anzar Ashraf

Published in: Archives of Virology | Issue 3/2013

Login to get access

Abstract

Dengue is an important arboviral disease of tropical and subtropical regions, with significant morbidity and mortality. Dengue virus is antigenically classified into four serotypes, which are further classified into 4–5 genotypes based on their genetic diversity. Since genotypes vary in their virulence, their detection and analysis of spatial and temporal transition are essential. We utilized sequence information from the E-NS1 gene region for molecular and phylogenetic characterization of dengue viruses isolated from dengue patients between 2007 and 2009. All four serotypes and multiple genotypes were detected, with predominance and emergence of DENV-1 genotype V. Phylogenetic analysis revealed the emergence of DENV-1 genotype V from India for the first time, which has replaced the earlier circulating genotype III and genotype I. The circulation of multiple genotypes and genotype replacement is critical, since genotypes vary in their virulence, and this should be a point of concern for healthcare agencies.
Literature
1.
2.
3.
4.
Zhang C, Mammen MP Jr, Chinnawirotpisan P, Klungthong C, Rodpradit P, Monkongdee P, Nimmannitya S, Kalayanarooj S, Holmes EC (2005) Clade replacements in dengue virus serotypes 1 and 3 are associated with changing serotype prevalence. J Virol 79(24):15123–15130PubMedCrossRef
5.
Nuegoonpipat A, Berlioz-Arthaud A, Chow V, Endy T, Lowry K, le Mai Q, Ninh TU, Pyke A, Reid M, Reynes JM, Su Yun ST, Thu HM, Wong SS, Holmes EC, Aaskov J (2004) Sustained transmission of dengue virus type 1 in the Pacific due to repeated introductions of different Asian strains. Virology 329(2):505–512. doi:10.​1016/​j.​virol.​2004.​08.​029 CrossRef
6.
Thu HM, Lowry KS, Jian L, Hlaing T, Holmes EC, Aaskov JG (2005) Lineage extinction and replacement in dengue type 1 virus populations is due to stochastic events rather than natural selection. Virology 336(2):163–172. doi:10.​1016/​j.​virol.​2005.​03.​018 CrossRef
7.
8.
Rico-Hesse R, Harrison LM, Salas RA, Tovar D, Nisalak A, Ramos C, Boshell J, de Mesa MT, Nogueira RM, da Rosa AT (1997) Origins of dengue type 2 viruses associated with increased pathogenicity in the Americas. Virology 230(2):244–251PubMedCrossRef
9.
Twiddy SS, Farrar JJ, Chau NV, Wills B, Gouldd EA, Gritsund T, Lloyde G, Holmes EC (2002) Phylogenetic relationships and differential selection pressures among genotypes of dengue-2 virus. Virology 298(1):63–72. doi:10.​1006/​viro.​2002.​1447 PubMedCrossRef
10.
Vasilakis N, Tesh RB, Weaver SC (2008) Sylvatic dengue virus type 2 activity in humans, Nigeria, 1966. Emerg Infect Dis 14(3):502–504PubMedCrossRef
11.
Wang E, Ni H, Xu R, Barrett AD, Watowich SJ, Gubler DJ, Weaver SC (2000) Evolutionary relationships of endemic/epidemic and sylvatic dengue viruses. J Virol 74(7):3227–3234PubMedCrossRef
12.
Lanciotti RS, Lewis JG, Gubler DJ, Trent DW (1994) Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol 75(Pt 1):65–75
13.
Wittke V, Robb TE, Thu HM, Nisalak A, Nimmannitya S, Kalayanrooj S, Vaughn DW, Endy TP, Holmes EC, Aaskov JG (2002) Extinction and rapid emergence of strains of dengue 3 virus during an interepidemic period. Virology 301(1):148–156PubMedCrossRef
14.
Lanciotti RS, Gubler DJ, Trent DW (1997) Molecular evolution and phylogeny of dengue-4 viruses. J Gen Virol 78(Pt 9):2279–2284PubMed
15.
16.
Risbud AR, Mehandale SM, Joshi GD, Banerjee K (1991) Recurrent outbreaks of dengue fever in rural areas of Maharashtra (an experience from Parbhani district). Indian J Virol 7:20–27
17.
Singh NP, Jhamb R, Agarwal SK, Gaiha M, Dewan R, Daga MK, Chakravarti A, Kumar S (2005) The 2003 Outbreak of Dengue fever in Delhi, India. Southeast Asian J Trop Med Public Health 36:1174–1178PubMed
18.
Chakravarti A, Arora R, Luxemburger C (2012) Fifty years of dengue in India. Trans R Soc Trop Med Hyg 106(5):273–282PubMedCrossRef
19.
Sabin AB (1952) Research on dengue during World War II. Am J Trop Med Hyg 1:30–50PubMed
20.
Sarkar JK, Pavri KM, Chatterjee SN, Chakravarty SK, Aanderson CR (1964) Virological and serological studies of cases of haemorrhagic fever in calcutta. Material collected by the Calcutta school of tropical medicine. Indian J Med Res 52:684–691PubMed
21.
Balaya S, Paul SD, D’Lima LV, Pavri KM (1969) Investigations on an outbreak of dengue in Delhi in 1967. Indian J Med Res 57(4):767–774PubMed
22.
23.
Diesh P, Pattanayak S, Singha P, Arora DD, Mathur PS, Ghosh TK, Mondal MM, Raghavan NGS (1972) An outbreak of dengue fever in Delhi–1970. J Commun Dis 4:13–18
24.
Rao CV, Bagchi SK, Pinto BD, Ilkal MA, Bharadwaj M, Shaikh BH, Dhanda V, Dutta M, Pavri KM (1985) The 1982 epidemic of dengue fever in Delhi. Indian J Med Res 82:271–275PubMed
25.
Kabra SK, Verma IC, Arora NK, Jain Y, Kalra V (1992) Dengue haemorrhagic fever in children in Delhi. Bull World Health Organ 70:105–108PubMed
26.
Broor S, Dar L, Sengupta S, Chakaraborty M, Wali JP, Biswas A, Kabra SK, Jain Y, Seth P (1997) Recent dengue epidemic in Delhi, India. In: Jean-François Saluzzo, Betty Dodet (eds) Factors in the Emergence of Arbovirus diseases. Elsevier Science, Amsterdam, pp 123–127
27.
Dar L, Gupta E, Narang P, Broor S (2006) Cocirculation of dengue serotypes, Delhi, India, 2003. Letter to the editor. Emerg Infect Dis 12(2):352–353PubMedCrossRef
28.
Kukreti H, Chaudhary A, Rautela RS, Anand R, Mittal V, Chhabra M, Bhattacharya D, Lal S, Rai A (2008) Emergence of an independent lineage of dengue virus type 1 (DENV-1) and its co-circulation with predominant DENV-3 during the 2006 dengue fever outbreak in Delhi. Int J Infect Dis 12(5):542–549PubMedCrossRef
29.
Singh UB, Maitra A, Broor S, Rai A, Pasha ST, Seth P (1999) Partial nucleotide sequencing and molecular evolution of epidemic causing dengue 2 strains. J Infect Dis 180(4):959–965PubMedCrossRef
30.
Dash PK, Parida MM, Saxena P, Kumar M, Rai A, Pasha ST, Jana AM (2004) Emergence and continued circulation of Dengue-2 (Genotype IV) virus strains in northern India. J Med Virol 74(2):314–322PubMedCrossRef
31.
Dash PK, Saxena P, Abhyankar A, Bhargava R, Jana AM (2005) Emergence of dengue virus type-3 in northern India. Southeast Asian J Trop Med Public Health 36(2):370–377PubMed
32.
Dash PK, Parida MM, Saxena P, Abhyankar A, Singh CP, Tewari KN, Jana AM, Sekhar K, Rao PVL (2006) Reemergence of dengue virus type-3 (subtype-III) in India: implications for increased incidence of DHF & DSS. Virol J 3:55PubMedCrossRef
33.
Chakravarti A, Matlani M, Kumar A (2010) Displacement of Dengue virus type 3 and type 2 by Dengue Virus type 1. Indian J Med Microbiol 28(4):412PubMedCrossRef
34.
Kumaria R, Chakravarti A (2005) Molecular detection and serotypic characterization of dengue viruses by single-tube multiplex reverse transcriptase–polymerase chain reaction. Diagn Microbial Infect Dis 52(4):311–316CrossRef
35.
Domingo C, Palacios G, Niedrig M, Cabrerizo M, Jabado O, Reyes N, Lipkin WI, Tenorio A (2004) A new tool for the diagnosis and molecular surveillance of dengue infections in clinical samples. WHO Dengue Bull 28:87–95
36.
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163PubMedCrossRef
37.
Goncalvez AP, Escalante AA, Pujol FH, Ludert JE, Tovar D et al (2002) Diversity and evolution of the envelope gene of dengue virus type 1. Virology 303(1):110–119PubMedCrossRef
38.
Kukreti H, Dash PK, Parida M, Chaudhary A, Saxena P, Rautela RS, Mittal V, Chhabra M, Bhattacharya D, Lal S, Rao PVL, Rai A (2009) Phylogenetic studies reveal existence of multiple lineages of a single genotype of DENV-1 (genotype III) in India during 1956–2007. Virology 6:1. doi:10.​1186/​1743-422X-6-1 CrossRef
39.
40.
Fu J, Tan BH, Yap EH, Chan YC, Tan YH (1992) Full-length cDNA sequence of dengue type 1 virus (Singapore strain S275/90). Virology 188(2):953–958PubMedCrossRef
41.
Singh UB, Seth P (2001) Use of nucleotide sequencing of the genomic cDNA fragments of the capsid/premembrane junction region for molecular epidemiology of dengue type 2 viruses. Southeast Asian J Trop Med Public Health 32(2):326–335PubMed
42.
Messer WB, Gubler DJ, Harris E, Sivananthan K, de Silva AM (2003) Emergence and global spread of a dengue serotype 3, subtype III virus. Emerg Infect Dis 9(7):800–809PubMedCrossRef
43.
Sierra B, Alegre R, Perez AB, Garcia G, Sturn-Ramirez K, Obasanjo O, Aguirre E, Alvarez M, Rodriguez-Roche R, Valdes L, Kanki P, Guzman MG (2007) HLA-A, -B, -C, and -DRB1 allele frequencies in Cuban individuals with antecedents of dengue 2 disease: advantages of the Cuban population for HLA studies of dengue virus infection. Hum Immunol 68(6):531–540
44.
Dash PK, Sharma S, Srivastava A, Santhosh SR, Parida MM, Neeraja M, Subbalaxmi MV, Lakshmi V, Rao PV (2010) Emergence of dengue virus type 4 (genotype I) in India. Epidemiol Infect 30:1–5. [Epub ahead of print]
45.
46.
Kuno G, Chang GJ, Tsuchiya KR, Karabatsos N, Cropp CB (1998) Phylogeny of the genus Flavivirus. J Virol 72(1):73–83
Metadata
Title
Genotypic characterization of dengue virus strains circulating during 2007-2009 in New Delhi
Authors
Anita Chakravarti
Mayank Singh Chauhan
Suman Kumar
Anzar Ashraf
Publication date
01-03-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 3/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1522-5

Other articles of this Issue 3/2013

Archives of Virology 3/2013 Go to the issue

Original Article

Characterization and whole genome sequences of the Rhodococcus bacteriophages RGL3 and RER2

Brief Report

Population structure of blackberry chlorotic ringspot virus in the United States

Original Article

Alteration of cell responses to PrPSc in prolonged cell culture and its effect on transmission of PrPSc to neural cells

Brief Report

Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B reassortment in nature with existing very virulent segment A

Annotated Sequence Record

Complete nucleotide sequences of two isolates of cherry green ring mottle virus from peach (Prunus persica) in China

Brief Report

Isolation of a novel herpesvirus from a Pacific white-sided dolphin
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discuss last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits