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Cancer Cell International
Published in: Cancer Cell International 1/2020

Open Access 01-12-2020 | Gastric Cancer | Primary research

miR-502-5p affects gastric cancer progression by targeting PD-L1

Authors: Wendao You, Xiaoyu Liu, Yang Yu, Chen Chen, Yujia Xiong, Yiting Liu, Yibin Sun, Chenhuan Tan, Hanshuo Zhang, Yadong Wang, Rui Li

Published in: Cancer Cell International | Issue 1/2020

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Abstract

Background

Studies have shown that miR-502-5p functions as a tumor suppressor and is associated with tumor growth and metastasis. This study intends to uncover the potential mechanism of miR-502-5p functioning as a tumor suppressor in gastric cancer.

Methods

Expression levels of miR-502-5p and PD-L1 were measured by using qRT-PCR. Cell proliferation abilities were examined by EDU incorporation assay. Cell migration, invasion and cell cycle analysis of cells were determined by transwell assay, transwell-matrigel assay and flow cytometry, respectively. The relationship between miR-502-5p expression and the overall survival of xenograft tumor mice was statistically analyzed. Bioinformatics analysis and luciferase reporter assays were applied to analyze the relationship between miR-502-5p and CD40, STAT3 or PD-L1. Expressions of CD40, STAT3 and PD-L1 at protein level were detected by western blot.

Results

The results showed that miR-502-5p was significantly downregulated in gastric cancer tumor tissues compared with adjacent normal tissues. Overexpression of miR-502-5p significantly attenuated the proliferation, migration/invasion and induced the G1 phase arrest of gastric cancer cells. Consistently, miR-502-5p suppressed tumor growth and metastasis in vivo. Mechanically, we demonstrated that miR-502-5p had inhibited the malignant behaviour of gastric cancer by down-regulating PD-L1 expression at transcriptional level and post-transcriptional levels.

Conclusions

These findings suggest that miR-502-5p acts as a tumor suppressor in gastric cancer (GC). MiR-502-5p/PD-L1 may be a novel therapeutic target in GC treatment.
Appendix
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Metadata
Title
miR-502-5p affects gastric cancer progression by targeting PD-L1
Authors
Wendao You
Xiaoyu Liu
Yang Yu
Chen Chen
Yujia Xiong
Yiting Liu
Yibin Sun
Chenhuan Tan
Hanshuo Zhang
Yadong Wang
Rui Li
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-020-01479-2

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