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Hepatology International
Published in: Hepatology International 1/2015

01-01-2015 | Original Article

FTY720, a sphingosine-1-phosphate (S1P) receptor modulator, protects sinusoid endothelial cells from radiation injury in vitro

Authors: Shu-Min Zhang, Yi-Xing Chen, Jing Sun, Lei Guo, Zhao-Chong Zeng

Published in: Hepatology International | Issue 1/2015

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Abstract

Purpose

To determine whether FTY720, a sphingosine-1-phosphate (S1P) receptor modulator, would protect sinusoid endothelial cells (SECs) from radiation injury in vitro.

Materials and methods

The effect of FTY720 on the viability of irradiated human liver SECs were examined by MTT assay or FACS analysis. The effect of FTY720 on the survival of hepatocellular carcinoma cell line, HepG2 and McA-RH7777, were determined by clonogenic assays. The activation of Akt pathway was tested by western bolt.

Results

FTY720 increases the survival of irradiated SECs; in contrast, it does not appear to be radioprotective of tumor cells. Furthermore, the activation of Akt pathway was confirmed in the protective effect of FTY720 on SECs.

Conclusion

These results suggest that FTY720 will be a potential therapeutic protector for the SEC apoptosis during RILD.
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Metadata
Title
FTY720, a sphingosine-1-phosphate (S1P) receptor modulator, protects sinusoid endothelial cells from radiation injury in vitro
Authors
Shu-Min Zhang
Yi-Xing Chen
Jing Sun
Lei Guo
Zhao-Chong Zeng
Publication date
01-01-2015
Publisher
Springer India
Published in
Hepatology International / Issue 1/2015
Print ISSN: 1936-0533
Electronic ISSN: 1936-0541
DOI
https://doi.org/10.1007/s12072-014-9590-0

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