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24-11-2023 | Food Allergy | Original Article

Semaphorin 3 a restores the ability of type 1 regulatory T cells to suppress food allergy

Authors: Panpan Gao, Shuo Song, Yanan Wang, Huazhen Liu, Xiangyu Wang, Qing shu, Pingchang Yang, Pengyuan Zheng

Published in: Immunologic Research | Issue 2/2024

Abstract

Food allergy (FA) is a common immune disorder that involves dysfunctional immune regulation. More remedies for restoring immune regulation are necessary. Semaphorin 3 A (Sema3a) is a secreted protein of the semaphorin family, which plays a role in immune responses at all stages. The objective of this study is to gain an understanding of how Sema3a can restore the immune regulatory abilities of type 1 regulatory T cells (Tr1 cells). In this study, blood samples were taken from patients with FA. Tr1 cells were purified from blood samples using flow cytometry cell sorting, using LAG3 and CD49b as surface markers. RNA sequencing was employed to examine the characteristics of Tr1 cells. We observed an exaggerated increase in ER stress in peripheral Tr1 cells of FA patients. Enforced expression of spliced X-box protein-1 (XBP1s, one of the key molecules in ER stress) resulted in suppression of interleukin (IL)-10 expression in CD4⁺ T cells. Eukaryotic initiation factor 2a (eIF2a) mediated the effects of XBP1 on suppressing IL-10 expression in Tr1 cells. The use of Sema3a resulted in a decrease in ER stress, and an increase in IL-10 expression in Tr1 cells of FA patients. Sema3a administration reduced experimental FA by increasing the number of Tr1 cells. In conclusion, IL-10 expression in Tr1 cells is disturbed by ER stress. Sema3a treatment restores the expression of IL-10 and the immunosuppressive capability of Tr1 cells.

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Title: Semaphorin 3 a restores the ability of type 1 regulatory T cells to suppress food allergy
Authors: Panpan Gao
Shuo Song
Yanan Wang
Huazhen Liu
Xiangyu Wang
Qing shu
Pingchang Yang
Pengyuan Zheng
Publication date: 24-11-2023
Publisher: Springer US
Keyword: Food Allergy
Published in: Immunologic Research / Issue 2/2024
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI: https://doi.org/10.1007/s12026-023-09437-6

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Semaphorin 3 a restores the ability of type 1 regulatory T cells to suppress food allergy

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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