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Magnetic Resonance Materials in Physics, Biology and Medicine

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Open Access 01-02-2019 | Research Article

Fluorine-19 MRI at 21.1 T: enhanced spin–lattice relaxation of perfluoro-15-crown-5-ether and sensitivity as demonstrated in ex vivo murine neuroinflammation

Authors: Sonia Waiczies, Jens T. Rosenberg, Andre Kuehne, Ludger Starke, Paula Ramos Delgado, Jason M. Millward, Christian Prinz, Joao dos Santos Periquito, Andreas Pohlmann, Helmar Waiczies, Thoralf Niendorf

Published in: Magnetic Resonance Materials in Physics, Biology and Medicine | Issue 1/2019

Abstract

Objective

Fluorine MR would benefit greatly from enhancements in signal-to-noise ratio (SNR). This study examines the sensitivity gain of ¹⁹F MR that can be practically achieved when moving from 9.4 to 21.1 T.

Materials and methods

We studied perfluoro-15-crown-5-ether (PFCE) at both field strengths (B₀), as a pure compound, in the form of nanoparticles (NP) as employed to study inflammation in vivo, as well as in inflamed tissue. Brains, lymph nodes (LNs) and spleens were obtained from mice with experimental autoimmune encephalomyelitis (EAE) that had been administered PFCE NPs. All samples were measured at both B₀ with 2D-RARE and 2D-FLASH using ¹⁹F volume radiofrequency resonators together. T₁ and T₂ of PFCE were measured at both B₀ strengths.

Results

Compared to 9.4 T, an SNR gain of > 3 was observed for pure PFCE and > 2 for PFCE NPs at 21.1 T using 2D-FLASH. A dependency of ¹⁹F T₁ and T₂ relaxation on B₀ was demonstrated. High spatially resolved ¹⁹F MRI of EAE brains and LNs at 21.1 T revealed signals not seen at 9.4 T.

Discussion

Enhanced SNR and T₁ shortening indicate the potential benefit of in vivo ¹⁹F MR at higher B₀ to study inflammatory processes with greater detail.

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Title: Fluorine-19 MRI at 21.1 T: enhanced spin–lattice relaxation of perfluoro-15-crown-5-ether and sensitivity as demonstrated in ex vivo murine neuroinflammation
Authors: Sonia Waiczies
Jens T. Rosenberg
Andre Kuehne
Ludger Starke
Paula Ramos Delgado
Jason M. Millward
Christian Prinz
Joao dos Santos Periquito
Andreas Pohlmann
Helmar Waiczies
Thoralf Niendorf
Publication date: 01-02-2019
Publisher: Springer International Publishing
Published in: Magnetic Resonance Materials in Physics, Biology and Medicine / Issue 1/2019
Print ISSN: 0968-5243
Electronic ISSN: 1352-8661
DOI: https://doi.org/10.1007/s10334-018-0710-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Fluorine-19 MRI at 21.1 T: enhanced spin–lattice relaxation of perfluoro-15-crown-5-ether and sensitivity as demonstrated in ex vivo murine neuroinflammation

Abstract

Objective

Materials and methods

Results

Discussion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and methods

Results

Discussion

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