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01-06-2017 | Original Article

First isolation and genetic characteristics of porcine sapeloviruses in Hunan, China

Authors: Taotao Yang, Runcheng Li, Wang Peng, Meng Ge, Binyu Luo, Tailong Qu, Xinglong Yu

Published in: Archives of Virology | Issue 6/2017

Abstract

Outbreaks of diarrhea in piglets cause serious economic consequences in China. Diarrhetic fecal samples from 20 Hunan farm piglets were tested and found to be positive for porcine epidemic diarrhea virus (PEDV) by RT-PCR, although incubation with porcine kidney (PK-15) cells failed to produce infectious PEDV. Four porcine sapelovirus (PSV) strains (designated as PSV-HuNs) were isolated from four of the samples. Genomic sequence analysis revealed open reading frames encoding polyproteins of 2,331 (HuN1, 2 and 3) and 2,332 (HuN4) amino acids. Homology comparisons of the VP1 gene of the four Hunan strains with previously reported PSV strains revealed nucleotide sequence identities ranging from 74.2 to 98.6%, and deduced amino acid sequence identities from 79.5 to 98%. Phylogenetic analyses based on full-length and partial VP1 gene sequences showed that 3 of the PSV-HuN strains (HuN2, 3 and 4) clustered within a clade distinct from HuN1 as well as from all PSVs previously isolated in China, thereby showing that genetic diversity exists within Chinese PSVs. In addition, recombination analysis among PSVs indicates that a recombinant (HuN2 strain) exist in China.

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ICTV (2015) International Committee on Taxonomy of Viruses. http://ictvonline.org/virusTaxonomy.asp. Accessed 11 Oct 2016

Chen J, Chen F, Zhou Q, Li W, Song Y, Pan Y, Zhang X, Xue C, Bi Y, Cao Y (2012) Complete genome sequence of a novel porcine Sapelovirus strain YC2011 isolated from piglets with diarrhea. J Virol 86:10898CrossRefPubMedPubMedCentral

Son K, Kim D, Kwon J, Choi J, Kang M, Belsham GJ, Cho K (2014) Full-length genomic analysis of Korean porcine sapelovirus strains. PLoS One 9:e107860CrossRefPubMedPubMedCentral

Lan D, Ji W, Yang S, Cui L, Yang Z, Yuan C, Hua X (2011) Isolation and characterization of the first Chinese porcine sapelovirus strain. Arch Virol 156:1567–1574CrossRefPubMed

Schock A, Gurrala R, Fuller H, Foyle L, Dauber M, Martelli F, Scholes S, Roberts L, Steinbach F, Dastjerdi A (2014) Investigation into an outbreak of encephalomyelitis caused by a neuroinvasive porcine sapelovirus in the United Kingdom. Vet Microbiol 172:381–389CrossRefPubMed

Abe M, Ito N, Sakai K, Kaku Y, Oba M, Nishimura M, Kurane I, Saijo M, Morikawa S, Sugiyama M (2011) A novel sapelovirus-like virus isolation from wild boar. Virus Genes 43:243–248CrossRefPubMed

Donin DG, de Arruda Leme R, Alfieri AF, Alberton GC, Alfieri AA (2014) First report of Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil. Trop Anim Health Prod 46:523–528CrossRefPubMed

Prodělalová J (2012) The survey of porcine teschoviruses, sapeloviruses and enteroviruses B infecting domestic pigs and wild boars in the Czech Republic between 2005 and 2011. Infect Genet Evol 12:1447–1451CrossRefPubMed

Son K, Kim D, Matthijnssens J, Kwon H, Park J, Hosmillo M, Alfajaro MM, Ryu E, Kim J, Kang M (2014) Molecular epidemiology of Korean porcine sapeloviruses. Arch Virol 159:1175–1180CrossRefPubMed

10.

Chen J, Chen F, Zhou Q, Li W, Chen Y, Song Y, Zhang X, Xue C, Bi Y, Cao Y (2014) Development of a minor groove binder assay for real-time PCR detection of porcine Sapelovirus. J Virol Methods 198:69–74CrossRefPubMed

11.

Johns MB, Paulus-Thomas JE (1989) Purification of human genomic DNA from whole blood using proteinase K treatment followed by phenol–chloroform extraction. Anal Biochem 180:276–278CrossRefPubMed

12.

Yu K, Pauls KP (1992) Optimization of the PCR program for RAPD analysis. Nucleic Acids Res 20:2606CrossRefPubMedPubMedCentral

13.

Rossmann MG, Arnold E, Erickson JW, Frankenberger EA, Griffith JP, Hecht H, Johnson JE, Kamer G, Luo M, Mosser AG (1985) Structure of a human common cold virus and functional relationship to other picornaviruses. Nature 317:145CrossRefPubMed

14.

Mateu MG (1995) Antibody recognition of picornaviruses and escape from neutralization: a structural view. Virus Res 38:1–24CrossRefPubMed

15.

Minor PD (1990) Antigenic structure of picornaviruses. picornaviruses. Springer, Berlin, pp 121–154CrossRef

16.

Oberste MS, Maher K, Kilpatrick DR, Pallansch MA (1999) Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. J Virol 73:1941–1948PubMedPubMedCentral

17.

Sozzi E, Barbieri I, Lavazza A, Lelli D, Moreno A, Canelli E, Bugnetti M, Cordioli P (2010) Molecular characterization and phylogenetic analysis of VP1 of porcine enteric picornaviruses isolates in Italy. Transbound Emerg Dis 57:434–442CrossRefPubMed

18.

Allen DJ, Gray JJ, Gallimore CI, Xerry J, Iturriza-Gómara M (2008) Analysis of amino acid variation in the P2 domain of the GII-4 norovirus VP1 protein reveals putative variant-specific epitopes. PLoS One 3:e1485CrossRefPubMedPubMedCentral

19.

Gao J, Chen J, Si X, Xie Z, Zhu Y, Zhang X, Wang S, Jiang S (2012) Genetic variation of the VP1 gene of the virulent duck hepatitis A virus type 1 (DHAV-1) isolates in Shandong province of China. Virol Sin 27:248–253CrossRefPubMed

20.

Haydon D, Lea S, Fry L, Knowles N, Samuel AR, Stuart D, Woolhouse ME (1998) Characterizing sequence variation in the VP1 capsid proteins of foot and mouth disease virus (serotype 0) with respect to virion structure. J Mol Evol 46:465–475CrossRefPubMed

21.

Kottaridi C, Bolanaki E, Mamuris Z, Stathopoulos C, Markoulatos P (2006) Molecular phylogeny of VP1, 2A, and 2B genes of echovirus isolates: epidemiological linkage and observations on genetic variation. Arch Virol 151:1117–1132CrossRefPubMed

22.

Weddell GN, Yansura DG, Dowbenko DJ, Hoatlin ME, Grubman MJ, Moore DM, Kleid DG (1985) Sequence variation in the gene for the immunogenic capsid protein VP1 of foot-and-mouth disease virus type A. Proc Natl Acad Sci 82:2618–2622CrossRefPubMedPubMedCentral

23.

Foo DGW, Alonso S, Phoon MC, Ramachandran NP, Chow VTK, Poh CL (2007) Identification of neutralizing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides. Virus Res 125:61–68CrossRefPubMed

24.

Horsington JJ, Gilkerson JR, Hartley CA (2012) Mapping B-cell epitopes in equine rhinitis B viruses and identification of a neutralising site in the VP1 C-terminus. Vet Microbiol 155:128–136CrossRefPubMed

25.

Inoue A, Choe Y, Kim BS (1994) Analysis of antibody responses to predominant linear epitopes of Theiler’s murine encephalomyelitis virus. J Virol 68:3324–3333PubMedPubMedCentral

26.

Kriegshäuser G, Wutz G, Lea S, Stuart D, Skern T, Kuechler E (2003) Model of the equine rhinitis A virus capsid: identification of a major neutralizing immunogenic site. J Gen Virol 84:2365–2373CrossRefPubMed

27.

Pulli T, Roivainen M, Hovi T, Hyypi T (1998) Induction of neutralizing antibodies by synthetic peptides representing the C terminus of coxsackievirus A9 capsid protein VP1. J Gen Virol 79:2249–2253CrossRefPubMed

28.

Xie QC, McCahon D, Crowther JR, Belsham GJ, McCullough KC (1987) Neutralization of foot-and-mouth disease virus can be mediated through any of at least three separate antigenic sites. J Gen Virol 68:1637–1647CrossRefPubMed

29.

Cano-Gómez C, García-Casado MA, Soriguer R, Palero F, Jiménez-Clavero MA (2013) Teschoviruses and sapeloviruses in faecal samples from wild boar in Spain. Vet Microbiol 165:115–122CrossRefPubMed

30.

Donin DG, Leme RDA, Alfieri AF, Alberton GC, Alfieri AA (2015) Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil. Pesquisa Vet Brasil 35:403–408CrossRef

31.

Oka T, Saif LJ, Marthaler D, Esseili MA, Meulia T, Lin C, Vlasova AN, Jung K, Zhang Y, Wang Q (2014) Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene. Vet Microbiol 173:258–269CrossRefPubMed

32.

Shibata I, Tsuda T, Mori M, Ono M, Sueyoshi M, Uruno K (2000) Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages. Vet Microbiol 72:173–182CrossRefPubMed

Title: First isolation and genetic characteristics of porcine sapeloviruses in Hunan, China
Authors: Taotao Yang
Runcheng Li
Wang Peng
Meng Ge
Binyu Luo
Tailong Qu
Xinglong Yu
Publication date: 01-06-2017
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 6/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3264-x

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