Abstract
Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) have been associated with enteric, respiratory, reproductive and neurological disorders. Although Brazil is the world’s fourth largest producer and exporter of pork, no information on the occurrence of PTV, PSV and EV-G infections is available for Brazilian pig herds. This study aimed to investigate the occurrence of Porcine enteric picornavirus infections in pig farms located in three distinct geographical regions of Brazil. Forty randomly selected diarrhoeic and normal consistency faeces of suckling (n = 22) and nursery (n = 18) pigs from farms located in 21 distinct cities of the Southern, Southeast, and Midwest regions of Brazil were evaluated by nested-RT-PCR assays. Suckling piglets presented the expected amplicon size for PTV (158 bp) and EV-G (313 bp) in single and mixed infections in 40.9 % (9/22) of the faecal samples. PSV amplicon (212 bp) was not detected in this age group. For nursery pigs, Porcine enteric picornaviruses amplicons were present in 77.8 % (14/18) of the faecal samples. PTV and EV-G were detected in single and mixed infections, while PSV was detected only in two samples in co-infection with PTV and EV-G in this age group. The Brazilian regions evaluated presented at least two of the tested viruses. Sequencing analysis revealed high similarities to the related viruses (95.3 to 99.2 % for PTV, 94.2 to 98.5 % for PSV and 86 to 100 % for EV-G). For the first time PTV, PSV and EV-G have been molecularly detected and characterised in pig faecal samples in Brazil.
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References
ABIPECS Brazilian Pork Industry and Exporter Association <http://www.abipecs.org.br/pt/estatisticas/mundial/producao-2.html> Accessed in: 28 July 2013
Alfieri, A.A., Parazzi, M.E., Takiuchi, E., Médici, K.C. and Alfieri, A.F., 2006. Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998–2002, Tropical Animal Health and Production, 38, 521--526
Bangari, D.S., Pogranichniy, R.M., Gillespie, T. and Stevenson, G.W., 2010. Genotyping of porcine teschovirus from nervous tissue of pigs with and without polioencephalomyelitis in Indiana, Journal of Veterinary Diagnostic Investigation, 22, 594--597
Boom, R., Sol, C.J., Salimans, M.M., Jansen, C.L., Wertheim-van Dillen, P.M. and van der Noordaa, J., 1990. Rapid and simple method for purification of nucleic acids, Journal of Clinical Microbiology, 28, 495--503
Boros, Á., Nemes, C., Pankovics, P., Kapusinszky, B., Delwart, E. and Reuter, G., 2012. Porcine teschovirus in wild boars in Hungary, Archives of Virology, 157, 1573--1578
Boros, Á., Pankovics, P. and Reuter, G., 2011. Characterization of a novel porcine enterovirus in domestic pig in Hungary, Infection, Genetics and Evolution, 11, 1096--1102
Buitrago, D., Cano-Gómez, C., Agüero, M., Fernandez-Pacheco, P., Gómez-Tejedor, C. and Jiménez-Clavero, M.Á., 2010. A survey of porcine picornaviruses and adenoviruses in fecal samples in Spain, Journal of Veterinary Diagnostic Investigation, 22, 763--766
Cano-Gómez, C., García-Casado, M.A., Soriguer, R., Palero, F. and Jiménez-Clavero, M.A., 2013. Teschoviruses and sapeloviruses in faecal samples from wild boar in Spain, Veterinary Microbiology, 165, 115--122
Cano-Gómez, C., Palero, F., Buitrago, M.D., García-Casado, M.A., Fernández-Pinero, J., Fernández-Pacheco, P., Agüero, M., Gómez-Tejedor, C. and Jiménez-Clavero, M.Á., 2011. Analyzing the genetic diversity of teschoviruses in Spanish pig populations using complete VP1 sequences, Infection, Genetics and Evolution, 11, 2144--2150
Deng, M.Y., Millien, M., Jacques-Simon, R., Flanagan, J.K., Bracht, A.J., Carrillo, C., Barrette, R.W., Fabian, A., Mohamed, F., Moran, K., Rowland, J., Swenson, S.L., Jenkins-Moore, M., Koster, L., Thomsen, B.V., Mayr, G., Pyburn, D., Morales, P., Shaw, J., Burrage, T., White, W., McIntosh, M.T. and Metwally, S., 2012. Diagnosis of Porcine teschovirus encephalomyelitis in the Republic of Haiti, Journal of Veterinary Diagnostic Investigation, 24, 671--678
ICTV International Committee on Taxonomy of Viruses <http://ictvonline.org/virusTaxonomy.asp?version=2012> Accessed in: 03 May 2013
Kaku, Y., Murakami, Y., Sarai, A., Wang, Y., Ohashi, S. and Sakamoto, K., 2007. Antigenic properties of porcine teschovirus 1 (PTV-1) Talfan strain and molecular strategy for serotyping of PTVs, Archives of Virology, 152, 929--940
Kaku, Y., Sarai, A. and Murakami, Y., 2001. Genetic reclassification of porcine enteroviruses, Journal of General Virology, 82, 417--424
Knowles, N.J., 1988. The association of group III porcine enteroviruses with epithelial tissue, Veterinary Record, 122, 441--442
Knowles, N.J., 2006. Porcine enteric picornaviruses, In: Straw, B.E., Zimmerman, J.J., D’Allaire, S. and Taylor, D.J. (Eds.), Diseases of swine, 9th ed. (Blackwell Publishing, Ames, Iowa), p. 337--345
Krumbholz, A., Dauber, M., Henke, A., Birch-Hirschfeld, E., Knowles, N.J., Stelzner, A. and Zell, R., 2002. Sequencing of porcine enterovirus groups II and III reveals unique features of both virus groups, Journal of Virology, 76, 5813--5821
Krumbholz, A., Wurm, R., Scheck, O., Birch-Hirschfeld, E., Egerer, R., Henke, A., Wutzler, P. and Zell, R., 2003. Detection of porcine teschoviruses and enteroviruses by LightCycler real-time PCR, Journal of Virological Methods, 113, 51--63
La Rosa, G., Muscillo, M., Di Grazia, A., Fontana, S., Iaconelli, M. and Tollis, M., 2006. Validation of RT-PCR assays for molecular characterization of porcine teschoviruses and enteroviruses, Journal of Veterinary Medicine, Series B, 53, 257--265
Lan, D., Ji, W., Yang, S., Cui, L., Yang, Z., Yuan, C. and Hua, X., 2011. Isolation and characterization of the first Chinese porcine sapelovirus strain, Archives of Virology, 156, 1567--1574
Lin, W., Cui, S. and Zell, R., 2012. Phylogeny and evolution of porcine teschovirus 8 isolated from pigs in China with reproductive failure, Archives of Virology, 157, 1387--1391
Moon, H.-J., Song, D., Seon, B.H., Kim, H.-K., Park, S.-J., An, D.-J., Kim, J.-M., Kang, B.-K. and Park, B.-K., 2012. Complete genome analysis of porcine enterovirus B isolated in Korea, Journal of Virology, 86, 10250
Prodělalová, J., 2012. The survey of porcine teschoviruses, sapeloviruses and enteroviruses B infecting domestic pigs and wild boars in the Czech Republic between 2005 and 2011, Infection, Genetics and Evolution, 12, 1447--1451
Salles, M.W.S., Scholes, S.F.E., Dauber, M., Strebelow, G., Wojnarowicz, C., Hassard, L., Acton, A.C. and Bollinger, T.K., 2011. Porcine teschovirus polioencephalomyelitis in western Canada, Journal of Veterinary Diagnostic Investigation, 23, 367--373
Shan, T., Li, L., Simmonds, P., Wang, C., Moeser, A. and Delwart, E., 2011. The fecal virome of pigs on a high-density farm, Journal of Virology, 85, 11697--11708
Sozzi, E., Barbieri, I., Lavazza, A., Lelli, D., Moreno, A., Canelli, E., Bugnetti, M. and Cordioli, P., 2010. Molecular characterization and phylogenetic analysis of VP1 of porcine enteric picornaviruses isolates in Italy, Transboundary and Emerging Diseases, 57, 434--442
Ventura, A., Gonzalez, W., Barrette, R., Swenson, S., Bracht, A., Rowland, J., Fabian, A., Moran, K., Mohamed, F., O’Hearn, E., Jenkins-Moore, M., Toms, D., Shaw, J., Morales, P., Pyburn, D., Carrillo, C., Mayr, G., McIntosh, M. and Deng, M., 2013. Virus and antibody diagnostics for swine samples of the Dominican Republic collected in regions near the border to Haiti, ISRN Virology, 2013, 1--7
Zell, R., Dauber, M., Krumbholz, A., Henke, A., Birch-Hirschfeld, E., Stelzner, A., Prager, D. and Wurm, R., 2001. Porcine teschoviruses comprise at least eleven distinct serotypes: molecular and evolutionary aspects, Journal of Virology, 75, 1620--1631
Zell, R., Krumbholz, A., Henke, A., Birch-Hirschfeld, E., Stelzner, A., Doherty, M., Hoey, E., Dauber, M., Prager, D. and Wurm, R., 2000. Detection of porcine enteroviruses by nRT–PCR: differentiation of CPE groups I–III with specific primer sets, Journal of Virological Methods, 88, 205--218
Zhang, W., Yang, S., Shen, Q., Ren, L., Shan, T., Wei, J., Cui, L. and Hua, X., 2012. Complete genome sequence of a novel porcine enterovirus strain in China, Journal of Virology, 86, 7008–7009
Acknowledgments
The authors are extremely grateful to Gefferson Almeida da Silva and Angelica Schmeing for their technical collaboration. We would like to thank the following Brazilian Institutes for financial support: the National Counsel of Scientific and Technological Development (CNPq), the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES), Financing of Studies and Projects (FINEP), and the Araucária Foundation (FAP/PR). Alfieri A.A. and Alfieri, A.F. are recipients of CNPq fellowships.
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Donin, D.G., de Arruda Leme, R., Alfieri, A.F. et al. First report of Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil. Trop Anim Health Prod 46, 523–528 (2014). https://doi.org/10.1007/s11250-013-0523-z
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DOI: https://doi.org/10.1007/s11250-013-0523-z