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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 4/2020

01-04-2020 | Fertility | Fertility Preservation

Cryopreservation of euploid blastocysts obtained after fertilization of in vitro matured ovarian tissue oocytes: a case report

Authors: Anastasia Kirillova, Evgeniya Kovalskaya, Olga Brovkina, Aleksey Ekimov, Ekaterina Bunyaeva, Marat Gordiev, Nona Mishieva, Tatiana Nazarenko, Aydar Abubakirov, Gennady Sukikh

Published in: Journal of Assisted Reproduction and Genetics | Issue 4/2020

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Abstract

With the increased rate of stable remission after gonadotoxic cancer treatment, new methods of fertility preservation are required in order to provide the best possible care for oncological patients. Here, we report an original case of euploid blastocyst cryopreservation after in vitro maturation of ovarian tissue oocytes (OTO IVM). Thirty-three oocytes were obtained from the ovarian tissue after ovariectomy in the breast cancer patient. Six out of 12 matured oocytes fertilized successfully and 3 blastocysts were formed. Genetic investigation for mutations associated with this type of malignancy found that the patient is not a carrier. Preimplantation genetic testing was performed only for aneuploidies and found all 3 blastocysts to be euploid and suitable for embryo transfer. Our study showed that the ovarian tissue oocytes matured in vitro have the potential for euploid blastocyst formation after ICSI which could be screened for aneuploidies and inherited mutations and then be vitrified in order to provide the best fertility preservation strategy for women with cancer.
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Metadata
Title
Cryopreservation of euploid blastocysts obtained after fertilization of in vitro matured ovarian tissue oocytes: a case report
Authors
Anastasia Kirillova
Evgeniya Kovalskaya
Olga Brovkina
Aleksey Ekimov
Ekaterina Bunyaeva
Marat Gordiev
Nona Mishieva
Tatiana Nazarenko
Aydar Abubakirov
Gennady Sukikh
Publication date
01-04-2020
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 4/2020
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-020-01729-2

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