Published in:
Open Access
01-08-2009 | Brief Report
Extended-spectrum beta-lactamases screening agar with AmpC inhibition
Authors:
N. Al Naiemi, J. L. Murk, P. H. M. Savelkoul, C. M. J. Vandenbroucke-Grauls, Y. J. Debets-Ossenkopp
Published in:
European Journal of Clinical Microbiology & Infectious Diseases
|
Issue 8/2009
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Excerpt
The serious increase in the prevalence of extended-spectrum beta-lactamases (ESBLs) worldwide creates a need for effective and easy to perform screening methods for detection [
1]. The use of an ESBL screening agar would allow rapid recognition and isolation of ESBL-producing bacteria. The currently available screening agars have low specificity, mainly due to growth of species with inducible AmpC beta-lactamases [
2,
3]. The inhibition of AmpC beta-lactamases by cloxacillin is used in ESBL confirmation tests [
4]; however, to the best of our knowledge, cloxacillin for ESBL screening has not been described. We developed an ESBL screening agar (ESA), which contains cloxacillin to inhibit growth of AmpC-producing species and vancomycin to inhibit growth of
Enterococci, and compared it with the commercially available, selective medium for screening of presumptive ESBL Enterobacteriaceae, namely, BLSE agar (AES Laboratory, France). …
