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Archives of Gynecology and Obstetrics

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01-04-2011 | General Gynecology

Expression of tyrosine kinase receptor B in eutopic endometrium of women with adenomyosis

Authors: Yaqin Huang, Wei Zheng, Lin Mu, Yuefang Ren, Xuejun Chen, Fang Liu

Published in: Archives of Gynecology and Obstetrics | Issue 4/2011

Abstract

Objective

Our study is to investigate whether tyrosine kinase receptor B (TrkB) is expressed in eutopic endometrium of women with adenomyosis and its association with clinical characteristics.

Methods

We collected endometrial tissues from 31 women with adenomyosis and 30 adenomyosis-free women undergoing surgery for benign indications. TrkB expression was assessed by immunohistochemistry and reverse-transcription polymerase chain reaction.

Results

Immunoreactive staining for TrkB was present as brown flocculent precipitate in the endometrial cells. The average level of TrkB protein (quantitation of immunostaining intensity) in secretory endometrial samples of women with adenomyosis was significantly higher than that in controls (p < 0.01). The average level of TrkB messenger RNA (mRNA) expression of women with adenomyosis was significantly higher than that of controls at secretory phase (p < 0.01). In addition, the immunostaining quantitation of TrkB protein was positively correlated with the serum CA125 (r = 0.308, p = 0.016) and dysmenorrhea (r = 0.393, p = 0.002).

Conclusions

Our study revealed elevation of TrkB protein and mRNA expression in the secretory endometrium of women with adenomyosis. Moreover, TrkB protein expression in human endometrium was positively correlated with the serum CA125 and dysmenorrhea. TrkB might contribute to the pathogenesis and progression of adenomyosis.

Mu L, Zheng W, Wang L, Chen XJ, Zhang X, Yang JH (2008) Alteration of focal adhesion kinase expression in eutopic endometrium of women with endometriosis. Fertil Steril 89(3):529–537PubMedCrossRef

Ren YF, Mu L, Ding XY, Zheng W (2010) Decreased expression of Beclin 1 in eutopic endometrium of women with adenomyosis. Arch Gynecol Obstet 282:401–406PubMedCrossRef

Mizumoto H, Saito T, Ashihara K, Nishimura M, Takehara M, Tanaka R et al (2002) Expression of matrix metalloproteinases in ovarian endometriomas: immuno-histochemical study and enzyme immunoassay. Life Sci 71:259–273PubMedCrossRef

Meresman GF, Vighi S, Buquet RA, Ortiz OC, Tesone M, Rumi LS (2000) Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis. Fertil Steril 74:760–766PubMedCrossRef

Douma S, Laar TV, Zevenhoven J, Meuwissen R, Van GE, Peeper DS (2004) Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB. Nature 430:1034–1040PubMedCrossRef

Satoh F, Mimata H, Nomura T, Fujita Y, Shin T, Sakamoto S et al (2001) Autocrine expression of neurotrophins and their receptors in prostate cancer. Int J Urol 8:S28–S34PubMedCrossRef

Pearse RN, Swendeman SL, Li Y, Rafii D, Hempstead BL (2005) A neurotrophin axis in myeloma: TrkB and BDNF promote tumor-cell survival. Blood 105(11):4429–4436PubMedCrossRef

Yu XH, Liu L, Cai B, He YY, Wan XP (2008) Suppression of anoikis by the neurotrophic receptor TrkB in human ovarian cancer. Cancer Sci 99(3):543–552PubMedCrossRef

Sclabas GM, Fujioka S, Schmidt C, Li ZK, Frederick WA, Yang WT et al (2005) Overexpression of tropomysin-related kinase B in metastatic human pancreatic cancer cells. Clin Cancer Res 11:440–449PubMed

10.

Braun DP, Ding J, Shaheen F, Willey JC, Rana N, Dmowski WP (2007) Quantitative expression of apoptosis-regulating genes in endometrium from women with and without endometriosis. Fertil Steril 87(2):263–268PubMedCrossRef

11.

Szymanowski K (2006) Apoptosis pattern in human endometrium in women with pelvic endometriosis. Eur J Obstet Gynecol Reprod Biol 68(2):343–350

12.

Dufournet C, Uzan C, Fauvet R, Cortez A, Siffroi JP, Darai E (2006) Expression of apoptosis-related proteins in peritoneal, ovarian and colorectal endometriosis. J Reprod Immunol 70(1):151–162PubMedCrossRef

13.

Noyes RW, Hertig AT, Rock J (1975) Dating the endometrial biopsy. Am J Obstet Gynecol 122:262–263PubMed

14.

Kelly AM (2001) The minimum clinically significant difference in visual analogue scale pain score does not differ with severity of pain. Emerg Med J 18:205–207PubMedCrossRef

15.

Elkas J, Armstrong A, Pohl J, Cuttitta F, Martínez A, Gray K (2000) Modulation of endometrial steroid receptors and growth regulatory genes by tamoxifen. Obstet Gynecol 95(5):697–703PubMedCrossRef

16.

Powitz AS, Zeitvogel A, Schreiner A, Baumann R (2001) In search of pathogenic mechanisms in endometriosis: the challenge for molecular cell biology. Curr Mol Med 1:655–664CrossRef

17.

Harada T, Kaponis A, Iwabe T, Taniguchi F, Makrydimas G, Sofikitis N et al (2004) Apoptosis in human endometrium and endometriosis. Hum Reprod Update 10:29–38PubMedCrossRef

18.

Cheng YM, Wang ST, Chou CY (2002) Serum CA-125 in preoperative patients at high risk for endometriosis. Obstet Gynecol 99(3):375–380PubMedCrossRef

19.

Matsuzaki S, Canis M, Barriere CV, Pouly JL, Tanguy OB, Llorca FP et al (2004) DNA microarray analysis of gene expression profiles in deep endometriosis using laser capture microdissection. Mol Hum Reprod 10:719–728PubMedCrossRef

20.

Matsuzaki S, Canis M, Pouly JL, Botchorishvili R, Déchelotte PJ, Mage G (2006) Differential expression of genes in eutopic and ectopic endometrium from patients with ovarian endometriosis. Fertil Steril 86:548–553PubMedCrossRef

21.

Fedele L, Bianchi S, Zanconato G, Portuese A, Raffaelli R (2001) Use of a levonorgestrel-releasing intrauterine device in the treatment of rectovaginal endometriosis. Fertil Steril 75(3):485–488PubMedCrossRef

22.

Matsuzaki S, Canis M, Pouly JL, Botchorishvili R, Déchelotte PJ, Mage G (2007) Both GnRH agonist and continuous oral progestin treatments reduce the expression of the tyrosine kinase receptor B and mu-opioid receptor in deep infiltrating endometriosis. Hum Reprod 22(1):124–128PubMedCrossRef

23.

Leyendecker G, Wildt L, Mall G (2009) The pathophysiology of endometriosis and adenomyosis: tissue injury and repair. Arch Gynecol Obstet 280:529–538PubMedCrossRef

24.

Wang XD, Ratnam J, Zou BD, England PM, Basbaum AI (2009) TrkB signaling is required for both the induction and maintenance of tissue and nerve injury-induced persistent pain. J Neurosci 29(17):5508–5515PubMedCrossRef

Title: Expression of tyrosine kinase receptor B in eutopic endometrium of women with adenomyosis
Authors: Yaqin Huang
Wei Zheng
Lin Mu
Yuefang Ren
Xuejun Chen
Fang Liu
Publication date: 01-04-2011
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 4/2011
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-010-1718-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Expression of tyrosine kinase receptor B in eutopic endometrium of women with adenomyosis

Abstract

Objective

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Results

Conclusions

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