Published in:
01-04-2011 | General Gynecology
Efficacy of anti-tumor necrosis factor therapy on endometriosis in an experimental rat model
Published in: Archives of Gynecology and Obstetrics | Issue 4/2011
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Objective
To show the efficacy of anti-tumor necrosis factor therapy (etanercept) for treating endometriosis in an experimental model.
Design
A randomized, placebo-controlled, blinded study using rat endometriosis model.
Setting
Experimental research center of Ankara Education and Research Hospital.
Animal(s)
Twenty-two Wistar female rats.
Intervention(s)
After peritoneal implantation of endometrial tissue, rats were randomized to two equal intervention groups: control and etanercept-treated groups. After measuring implant volume, blood and peritoneal fluid samples were obtained. Vehicle treatments of 2 mL saline to rats in control and 0.4 mg/kg etanercept SC once weekly were administered in treatment group. Four weeks later, a third laparotomy was performed to remeasure implant volumes, blood, and peritoneal fluid samples.
Main outcome measure(s)
To compare spherical volume, peritoneal fluid and serum levels of VEGF, IL-6, and TNF-α between groups.
Result(s)
There was a significant difference in spherical volume between control [131.0 (60.3–501.2)] and treatment groups [72.8 (31.2–149.6)] (p < 0.025). In etanercept-treated group, a significant difference was found between peritoneal fluid and serum levels of VEGF, IL-6, and TNF-α (p < 0.01).
Conclusion(s)
These results indicate that etanercept was found to effectively reduce the development of endometriosis.