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Lasers in Medical Science
Published in: Lasers in Medical Science 1/2015

01-01-2015 | Original Article

Expression of mPGES-1 and IP mRNA is reduced by LLLT in both subplantar and brain tissues in the model of peripheral inflammation induced by carrageenan

Authors: Luciene Reginato Chagas, José Antonio Silva Jr., Juliana de Almeida Pires, Maricilia S. Costa

Published in: Lasers in Medical Science | Issue 1/2015

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Abstract

The increase in PGE2 production by microsomal PGE synthase-1 (mPGES-1) in CNS contributes to the severity of the inflammatory and pain responses in the model of edema formation and hyperalgesia induced by carrageenan. PGI2, alike to PGE2, plays an important role in the inflammation. Low-level laser therapy (LLLT) has been used in the treatment of inflammatory pathologies, reducing both pain and the acute inflammatory process. In this work, we studied the effect of LLLT on the expression of both mPGES-1 and IP messenger RNA (mRNA), in either subplantar or total brain tissues obtained from rats submitted to model of edema formation and hyperalgesia induced by carrageenan administration. The test sample consisted of 30 rats divided into five groups: A1 (control—saline), A2 (carrageenan—0.5 mg/paw), A3 (carrageenan—0.5 mg/paw + LLLT), A4 (carrageenan—1.0 mg/paw), and A5 (carrageenan—1.0 mg/paw + LLLT). The animals from groups A3 and A5 were irradiated 1 h after induction of inflammation by carrageenan injection. Continuous-wave red laser with wavelengths of 660 nm and dose of 7.5 J/cm2 was used. Six hours after carrageenan-induced inflammation, mPGES-1 and prostacyclin receptor (IP) mRNA expression were significantly increased both in subplantar and brain tissues. LLLT was able to reduce both mPGES-1 and IP mRNA expression in subplantar and brain tissues. We suggest that LLLT is able to reduce both inflammation and hyperalgesia observed in the model of edema formation and hyperalgesia induced by carrageenan, by a mechanism involving the decrease in the expression of both mPGES-1 and IP.
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Metadata
Title
Expression of mPGES-1 and IP mRNA is reduced by LLLT in both subplantar and brain tissues in the model of peripheral inflammation induced by carrageenan
Authors
Luciene Reginato Chagas
José Antonio Silva Jr.
Juliana de Almeida Pires
Maricilia S. Costa
Publication date
01-01-2015
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 1/2015
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-014-1622-4

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