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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2017

Open Access 01-12-2017 | Research

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach

Authors: Joanna Budna, Marta Rybska, Sylwia Ciesiółka, Artur Bryja, Sylwia Borys, Wiesława Kranc, Katarzyna Wojtanowicz-Markiewicz, Michal Jeseta, Ewa Sumelka, Dorota Bukowska, Paweł Antosik, Klaus P. Brüssow, Małgorzata Bruska, Michał Nowicki, Maciej Zabel, Bartosz Kempisty

Published in: Reproductive Biology and Endocrinology | Issue 1/2017

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Abstract

Background

The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduction pathways crucial for folliculogenesis and oogenesis. Transforming growth factor beta signaling includes several molecules that are involved in the regulation of oogenesis and embryo growth, including bone morphogenetic protein (BMP). However, the BMP-related gene expression profile in oocytes at different maturational stages requires further investigation.

Methods

Oocytes were isolated from pubertal crossbred Landrace gilts follicles, selected with a use of BCB staining test and analyzed before and after in vitro maturation. Gene expression profiles were examined using an Affymetrix microarray approach and validated by RT-qPCR. Database for Annotation, Visualization, and Integrated Discovery (DAVID) software was used for the extraction of the genes belonging to a BMP-signaling pathway ontology group.

Results

The assay revealed 12,258 different transcripts in porcine oocytes, among which 379 genes were down-regulated and 40 were up-regulated. The DAVID database indicated a “BMP signaling pathway” ontology group, which was significantly regulated in both groups of oocytes. We discovered five up-regulated genes in oocytes before versus after in vitro maturation (IVM): chordin-like 1 (CHRDL1), follistatin (FST), transforming growth factor-beta receptor-type III (TGFβR3), decapentaplegic homolog 4 (SMAD4), and inhibitor of DNA binding 1 (ID1).

Conclusions

Increased expression of CHRDL1, FST, TGFβR3, SMAD4, and ID1 transcripts before IVM suggested a subordinate role of the BMP signaling pathway in porcine oocyte maturational competence. Conversely, it is postulated that these genes are involved in early stages of folliculogenesis and oogenesis regulation in pigs, since in oocytes before IVM increased expression was observed.
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Metadata
Title
Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach
Authors
Joanna Budna
Marta Rybska
Sylwia Ciesiółka
Artur Bryja
Sylwia Borys
Wiesława Kranc
Katarzyna Wojtanowicz-Markiewicz
Michal Jeseta
Ewa Sumelka
Dorota Bukowska
Paweł Antosik
Klaus P. Brüssow
Małgorzata Bruska
Michał Nowicki
Maciej Zabel
Bartosz Kempisty
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2017
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/s12958-017-0261-6

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