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Journal of Mammary Gland Biology and Neoplasia

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Open Access 01-12-2024 | Estrogens | Research

Single-Cell Transcription Mapping of Murine and Human Mammary Organoids Responses to Female Hormones

Authors: Jenelys Ruiz Ortiz, Steven M. Lewis, Michael Ciccone, Deeptiman Chatterjee, Samantha Henry, Adam Siepel, Camila O. dos Santos

Published in: Journal of Mammary Gland Biology and Neoplasia | Issue 1/2024

Abstract

During female adolescence and pregnancy, rising levels of hormones result in a cyclic source of signals that control the development of mammary tissue. While such alterations are well understood from a whole-gland perspective, the alterations that such hormones bring to organoid cultures derived from mammary glands have yet to be fully mapped. This is of special importance given that organoids are considered suitable systems to understand cross species breast development. Here we utilized single-cell transcriptional profiling to delineate responses of murine and human normal breast organoid systems to female hormones across evolutionary distinct species. Collectively, our study represents a molecular atlas of epithelial dynamics in response to estrogen and pregnancy hormones.

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Title: Single-Cell Transcription Mapping of Murine and Human Mammary Organoids Responses to Female Hormones
Authors: Jenelys Ruiz Ortiz
Steven M. Lewis
Michael Ciccone
Deeptiman Chatterjee
Samantha Henry
Adam Siepel
Camila O. dos Santos
Publication date: 01-12-2024
Publisher: Springer US
Keyword: Estrogens
Published in: Journal of Mammary Gland Biology and Neoplasia / Issue 1/2024
Print ISSN: 1083-3021
Electronic ISSN: 1573-7039
DOI: https://doi.org/10.1007/s10911-023-09553-x

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