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Digestive Diseases and Sciences
Published in: Digestive Diseases and Sciences 12/2020

01-12-2020 | Estrogens | Original Article

Estrogen Regulates Duodenal Calcium Absorption Through Differential Role of Estrogen Receptor on Calcium Transport Proteins

Authors: Xubiao Nie, Hai Jin, Guorong Wen, Jingyu Xu, Jiaxing An, Xuemei Liu, Rui Xie, Biguang Tuo

Published in: Digestive Diseases and Sciences | Issue 12/2020

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Abstract

Background and Aims

Intestinal calcium absorption from the diet plays important role in maintaining calcium homeostasis in the body. Estrogen exerts wide physiological and pathological effects in the human. Previous studies have shown that estrogen is involved in the intestinal calcium absorption. In this study, we made investigation on the mechanism of estrogen action on duodenal calcium absorption.

Methods

The experiments were performed in mice, human, and human duodenal epithelial cells, SCBN cells. Murine duodenal calcium absorption was measured by using single pass perfusion of the duodenum in vivo. The calcium absorption of SCBN cells was evaluated by calcium imaging system. The expression of calcium transport proteins, transient receptor potential cation channel (TRPV6) and plasma membrane calcium pump (PMCA1b), in the duodenum or SCBN cells were analyzed by western blot.

Results

The duodenal calcium absorption in ovariectomized mice was significantly decreased, compared with control female mice, which returned to control level after 17β-estradiol replacement treatment. Estrogen regulated the expressions of TRPV6 and PMCA1b in murine and human duodenal mucosae and SCBN cells. The further results from SCBN cells showed that 17β-estradiol regulated calcium influx through the respective effects of estrogen receptor (ER) ɑ and β on TRPV6 and PMCA1b.

Conclusion

Estrogen regulates duodenal calcium absorption through differential role of ERɑ and ERβ on duodenal epithelial cellular TRPV6 and PMCA1b. The study further elucidates the mechanism of estrogen on the regulation of intestinal calcium absorption.
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Metadata
Title
Estrogen Regulates Duodenal Calcium Absorption Through Differential Role of Estrogen Receptor on Calcium Transport Proteins
Authors
Xubiao Nie
Hai Jin
Guorong Wen
Jingyu Xu
Jiaxing An
Xuemei Liu
Rui Xie
Biguang Tuo
Publication date
01-12-2020
Publisher
Springer US
Published in
Digestive Diseases and Sciences / Issue 12/2020
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI
https://doi.org/10.1007/s10620-020-06076-x

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