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Respiratory Research
Published in: Respiratory Research 1/2011

Open Access 01-12-2011 | Research

Epilysin (matrix metalloproteinase-28) contributes to airway epithelial cell survival

Authors: Anne M Manicone, Susanna Harju-Baker, Laura K Johnston, Ann J Chen, William C Parks

Published in: Respiratory Research | Issue 1/2011

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Abstract

MMP28 is constitutively expressed by epithelial cells in many tissues, including the respiratory epithelium in the lung and keratinocytes in the skin. This constitutive expression suggests that MMP28 may serve a role in epithelial cell homeostasis. In an effort to determine its function in epithelial cell biology, we generated cell lines expressing wild-type or catalytically-inactive mutant MMP28 in two pulmonary epithelial cell lines, A549 and BEAS-2B. We observed that over-expression of MMP28 provided protection against apoptosis induced by either serum-deprivation or treatment with a protein kinase inhibitor, staurosporine. Furthermore, we observed increased caspase-3/7 activity in influenza-infected lungs from Mmp28 -/- mice compared to wild-type mice, and this activity localized to the airway epithelium but was not associated with a change in viral load. Thus, we have identified a novel role of MMP28 in promoting epithelial cell survival in the lung.
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Metadata
Title
Epilysin (matrix metalloproteinase-28) contributes to airway epithelial cell survival
Authors
Anne M Manicone
Susanna Harju-Baker
Laura K Johnston
Ann J Chen
William C Parks
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Respiratory Research / Issue 1/2011
Electronic ISSN: 1465-993X
DOI
https://doi.org/10.1186/1465-9921-12-144

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