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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2023

Open Access 01-12-2023 | Encephalitis | Research

H3K27me3 of Rnf19a promotes neuroinflammatory response during Japanese encephalitis virus infection

Authors: Shuo Zhu, Mengying Tao, Yunchuan Li, Xugang Wang, Zikai Zhao, Yixin Liu, Qi Li, Qiuyan Li, Yanbo Lu, Youhui Si, Shengbo Cao, Jing Ye

Published in: Journal of Neuroinflammation | Issue 1/2023

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Abstract

Histone methylation is an important epigenetic modification that affects various biological processes, including the inflammatory response. In this study, we found that infection with Japanese encephalitis virus (JEV) leads to an increase in H3K27me3 in BV2 microglial cell line, primary mouse microglia and mouse brain. Inhibition of H3K27me3 modification through EZH2 knockdown and treatment with EZH2 inhibitor significantly reduces the production of pro-inflammatory cytokines during JEV infection, which suggests that H3K27me3 modification plays a crucial role in the neuroinflammatory response caused by JEV infection. The chromatin immunoprecipitation-sequencing (ChIP-sequencing) assay revealed an increase in H3K27me3 modification of E3 ubiquitin ligases Rnf19a following JEV infection, which leads to downregulation of Rnf19a expression. Furthermore, the results showed that Rnf19a negatively regulates the neuroinflammatory response induced by JEV. This is achieved through the degradation of RIG-I by mediating its ubiquitination. In conclusion, our findings reveal a novel mechanism by which JEV triggers extensive neuroinflammation from an epigenetic perspective.
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Metadata
Title
H3K27me3 of Rnf19a promotes neuroinflammatory response during Japanese encephalitis virus infection
Authors
Shuo Zhu
Mengying Tao
Yunchuan Li
Xugang Wang
Zikai Zhao
Yixin Liu
Qi Li
Qiuyan Li
Yanbo Lu
Youhui Si
Shengbo Cao
Jing Ye
Publication date
01-12-2023
Publisher
BioMed Central
Keyword
Encephalitis
Published in
Journal of Neuroinflammation / Issue 1/2023
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/s12974-023-02852-4

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