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Journal of Infection and Chemotherapy
Published in: Journal of Infection and Chemotherapy 6/2011

01-12-2011 | Note

Electron microscopic study of the effects of antimicrobial agents on the cellular architecture of Leptospira

Authors: Antara Chakraborty, Akemi Takade, Sharon Y. A. M. Villanueva, Shin-ichi Yoshida

Published in: Journal of Infection and Chemotherapy | Issue 6/2011

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Abstract

Morphological changes of Leptospira induced by ampicillin, streptomycin, or ciprofloxacin were studied by transmission electron microscopy after staining with uranyl acetate. Irregularities in coiling, loss of hooks, spherical swellings, membrane vesicle formation, disruption of outer membrane, exposure and detachment of flagella, penetration of dye, and lysis of cells were observed. These changes appeared in a time-dependent manner and appeared to be associated with the sites of action of antimicrobial agents. This is the first report delineating the action of aminoglycoside and new quinolone on the morphology of Leptospira cells.
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Adler B, Faine S. Leptospira. In: Borriello SP, Murray PR, Funke G, editors. Topley and Wilson’s microbiology and microbiological infections, 10th edn. Bacteriology, vol 2. London: Arnold; 2005. p. 1865–1884
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Bystricky V, Ladzianska K, Halasa M. Electron microscopy of the action of polymyxin on leptospirae. J Bacteriol. 1964;84:864–5.
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Chakraborty A, Miyahara S, Villanueva SYAM, Gloriani NG, Yoshida S. In vitro sensitivity and resistance of 46 Leptospira strains isolated from rats of the Philippines to 14 antimicrobial agents. Antimicrob Agents Chemother. 2010;54:5403–5.PubMedCrossRef
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Villanueva SYAM, Ezoe H, Baterna RA, Yanagihara Y, Muto M, Koizumi N, et al. Serologic and molecular studies of Leptospira and leptospirosis among rats in the Philippines. Am J Trop Med Hyg. 2010;82:889–98.PubMedCrossRef
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Metadata
Title
Electron microscopic study of the effects of antimicrobial agents on the cellular architecture of Leptospira
Authors
Antara Chakraborty
Akemi Takade
Sharon Y. A. M. Villanueva
Shin-ichi Yoshida
Publication date
01-12-2011
Publisher
Springer Japan
Published in
Journal of Infection and Chemotherapy / Issue 6/2011
Print ISSN: 1341-321X
Electronic ISSN: 1437-7780
DOI
https://doi.org/10.1007/s10156-011-0258-6

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