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01-02-2020 | Electromyographic | Original Article

Leuprolide Acetate, a GnRH Agonist, Improves the Neurogenic Bowel in Ovariectomized Rats with Spinal Cord Injury

Authors: Moisés Altamira-Camacho, Daniel Medina-Aguiñaga, Yolanda Cruz, Denisse Calderón-Vallejo, Kalman Kovacs, Fabio Rotondo, J. Luis Quintanar

Published in: Digestive Diseases and Sciences | Issue 2/2020

Abstract

Background

Electromyographic studies have shown that external anal sphincter activity is modified in response to distension in animals with spinal cord injury. Gonadotropin-releasing hormone and its agonist leuprolide acetate have neurotrophic properties in animals with spinal cord injury.

Aim

This study was to determine the effects of leuprolide acetate treatment on electromyographic activity of the external anal sphincter and anorectal manometry in ovariectomized rats with spinal cord injury.

Methods

Adult ovariectomized rats were divided in three groups: (a) sham of spinal cord injury, (b) spinal cord injury treated with saline solution, and (c) spinal cord injury treated with leuprolide acetate. The spinal cord injury was induced by clamping at level T9. Leuprolide acetate dosage of 10 μg/kg was proctored intramuscular for 5 weeks, commencing the day after the lesion. Electromyography of the external anal sphincter, anorectal manometry, and volume of the cecum were evaluated in all groups.

Results

The electromyographic study of the external anal sphincter activity showed a significant improvement in injured rats treated with leuprolide acetate. Manometric analysis and cecum volume data obtained in animals with leuprolide acetate were very similar to those found in the sham group.

Conclusions

These results demonstrate that leuprolide acetate treatment improves the neurogenic colon in ovariectomized rats with spinal cord injury.

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Title: Leuprolide Acetate, a GnRH Agonist, Improves the Neurogenic Bowel in Ovariectomized Rats with Spinal Cord Injury
Authors: Moisés Altamira-Camacho
Daniel Medina-Aguiñaga
Yolanda Cruz
Denisse Calderón-Vallejo
Kalman Kovacs
Fabio Rotondo
J. Luis Quintanar
Publication date: 01-02-2020
Publisher: Springer US
Keywords: Electromyographic
Leuprolide
Published in: Digestive Diseases and Sciences / Issue 2/2020
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-019-05783-4

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Abstract

Background

Aim

Methods

Results

Conclusions

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