Top

Published in:

01-04-2009 | Original Paper

Effects of the nonsugar fraction of brown sugar on chronic ultraviolet B irradiation-induced photoaging in melanin-possessing hairless mice

Authors: Maho Sumiyoshi, Teruaki Hayashi, Yoshiyuki Kimura

Published in: Journal of Natural Medicines | Issue 2/2009

Abstract

Brown sugar has been used traditionally for the treatment of skin trouble as a component of soaps or lotions. Symptoms of aging including wrinkles and pigmentation develop earlier in sun-exposed skin than unexposed skin, a phenomenon referred to as photoaging. Ultraviolet B (UVB) radiation is one of the most important environmental factors influencing photoaging. The aim of this study was to clarify whether the nonsugar fraction of brown sugar prevents chronic UVB-induced aging of the skin using melanin-possessing hairless mice. The nonsugar fraction (1% or 3% solution, 50 μl/mouse) was applied topically to the dorsal region every day for 19 weeks. Both solutions prevented an increase in skin thickness and reduction in skin elasticity caused by the UVB. The 3% solution also prevented wrinkles and melanin pigmentation as well as increases in the diameter and length of skin blood vessels. Increases in the expression of matrix metalloproteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) in UVB-irradiated skin was inhibited by the nonsugar fraction. Prevention of UVB-induced aging of the skin by topical application of the nonsugar fraction of brown sugar may be due to inhibition of increases in MMP-2 and VEGF expression.

Kimura Y, Okuda H, Arichi S (1984) Effects of non-sugar fraction in black sugar on lipid and carbohydrate metabolism: Part I. Planta Med 50:465–468PubMedCrossRef

Kimura Y, Okuda H, Shoji N, Takemoto T, Arichi S (1984) Effects of non-sugar fraction in black sugar on lipid and carbohydrate metabolism: Part II—New compounds inhibiting elevation of plasma insulin. Planta Med 50:469–473PubMedCrossRef

Sams WM, Smith J (1961) The histochemistry of chronically sun damaged skin. J Invest Dermatol 37:447–452PubMed

Smith J, Davidson EA, Sams WM, Clark RD (1962) Alterations in human dermal connective tissue with age and chronic sun damage. J Invest Dermatol 39:347–350PubMed

Uitto J, Fazio MJ, Olsen DR (1989) Molecular mechanisms of cutaneous aging age-associated connective tissue alterations in the dermis. J Am Acad Dermatol 21:614–622PubMedCrossRef

Bissett DL, Hannon DP, Orr TV (1987) An animal model of solar-aged skin: histological, physical, and visible changes in UV-irradiated hairless mouse skin. Photochem Photobiol 46:367–378PubMedCrossRef

de Gruiji FR, Sterenborg HJ, Forbes PD, Davies RE, Cole C, Kelfkens G, van Weelden H, Slaper H, van der Leun JC (1993) Wavelength dependence of skin induction by ultraviolet irradiation of albino hairless mice. Cancer Res 53:53–60

Beissert S, Schwarz T (1999) Mechanisms involved in ultraviolet light-induced immunosuppression. J Investg Dermatol Symp Proc 4:61–64CrossRef

Fisher GJ, Wang ZQ, Fatta SC, Varani J, Kang S, Voorhees JJ (1997) Pathophysiology of premature skin aging induced by ultraviolet light. N Engl Med 337:1419–1428CrossRef

10.

Inomata S, Matsunaga Y, Amano S, Takada K, Kobayashi K, Tsunenaga M, Nishiyama T, Kohno Y, Fukuda M (2003) Possible involvement of gelatinases in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse. J Invest Dermatol 120:128–134PubMedCrossRef

11.

Amano S, Akutsu N, Matsunaga Y, Kadoya K, Nishiyama T, Champliaud M-F, Burgeson RE, Adachi E (2001) Importance of balance between extracellular matrix synthesis and degradation in basement membrane formation. Exp Cell Res 271:249–262PubMedCrossRef

12.

Birkedal-Hansen H (1995) Protelytic remodeling of extracellular matrix. Curr Opin Cell Biol 7:728–735PubMedCrossRef

13.

Cox NH, Diffey BL, Farr PM (1992) The relationship between chronological age and the erythemal response to ultraviolet B radiation. Br J Dermatol 98:248–254

14.

Kripke ML (1994) Ultraviolet radiation and immunology: something new under the sun-presidential address. Cancer Res 54:6102–6105PubMed

15.

Pearse AD, Gaskell SA, Marks R (1987) Epidermal changes in human skin following irradiation with either UVB or UVA. J Invest Dermatol 88:83–87PubMedCrossRef

16.

Berton TR, Mitchell DL, Fischer SM, Locniskar MF (1997) Epidermal proliferation but not quantity of DNA photodamage is correlated with UV-induced mouse skin carcinogenesis. J Invest Dermatol 109:340–347PubMedCrossRef

17.

Kramer M, Sachsenmaier C, Herrlich P, Rahmsdorf HJ (1993) UV irradiation-induced interleukin-1 and basic fibroblast growth factor synthesis and release mediate part of the UV response. J Biol Chem 268:6734–6741PubMed

18.

Strickland I, Rhodes LE, Flanagan BF, Friedmann PS (1997) TNF-α and IL-8 are upregulated in the epidermis of normal human skin after UVB exposure: correlation with neutrophil accumulation and E-selectin expression. J Invest Dermatol 108:763–768PubMedCrossRef

19.

Bielenberg DR, Bucana CD, Sanchez R, Donawho CK, Kripke ML, Fidler IJ (1998) Molecular regulation of UVB-induced cutaneous angiogenesis. J Invest Dertmatol 111:864–872CrossRef

20.

Yano K, Oura H, Detmar M (2002) Targeted over expression of the angiogenesis inhibitor thrombospondin-1 in the epidermis of transgenic mice prevents ultraviolet-B-induced angiogenesis and cutaneous photo-damage. J Invest Dermatol 118:800–805PubMedCrossRef

21.

Yano K, Kajiya K, Ishikawa M, Hong Y-K, Miyakawa T, Detmar M (2004) Ultraviolet B-induced skin angiogenesis is associated with switch in the balance of vascular endothelial growth factor and thrombospondin-1 expression. J Invest Dermatol 122:201–208PubMedCrossRef

22.

Bein K, Simons M (2000) Thrombospondin type 1 repeats interact with matrix metalloproteinase 2. Regulation of metalloproteinase activity. J Biol Chem 275:32167–32173PubMedCrossRef

23.

Taraboletti G, Sonzogni L, Vergani V, Hosseini G, Ceruti R, Ghilardi C, Bastone A, Toschi E, Borsotti P, Scanziani E, Giavazzi R, Pepper MS, Stetler-Stevenson WG, Bani MR (2000) Posttranscriptional stimulation of endothelial cell matrix metalloproteinases 2 and 1 by endothelioma cells. Exp Cell Res 258:384–394PubMedCrossRef

24.

Rodriguez-Manzaneque JC, Lane TF, Ortega MA, Hynes RO, Lawler J, Iruela-Arispe ML (2001) Thrombospondin-1 suppresses spontaneous tumor growth and inhibits activation of matrix metalloproteinase-9 and mobilization of vascular endothelial growth factor. Proc Natl Acad Sci USA 98:12485–12490PubMedCrossRef

Title: Effects of the nonsugar fraction of brown sugar on chronic ultraviolet B irradiation-induced photoaging in melanin-possessing hairless mice
Authors: Maho Sumiyoshi
Teruaki Hayashi
Yoshiyuki Kimura
Publication date: 01-04-2009
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 2/2009
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-008-0301-9

2024 ASCO Annual Meeting

Springer Medicine

Effects of the nonsugar fraction of brown sugar on chronic ultraviolet B irradiation-induced photoaging in melanin-possessing hairless mice

Abstract

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2009

Anti-inflammatory and analgesic components from “hierba santa,” a traditional medicine in Peru

Two new neolignan glycosides from leaves of Osmanthus heterophyllus

In vitro antiproliferative characteristics of flavonoids and diazepam on SNU-C4 colorectal adenocarcinoma cells

Survey of Glycyrrhizae Radix resources in Mongolia: chemical assessment of the underground part of Glycyrrhiza uralensis and comparison with Chinese Glycyrrhizea Radix

Confirmation of the antispasmodic effect of shakuyaku-kanzo-to (TJ-68), a Chinese herbal medicine, on the duodenal wall by direct spraying during endoscopic retrograde cholangiopancreatography

Appraisal of immunomodulatory potential of Spirulina fusiformis: an in vivo and in vitro study