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01-09-2016 | Brief Report

Effects of the led therapy on the global DNA methylation and the expression of Dnmt1 and Dnmt3a genes in a rat model of skin wound healing

Authors: Marcus Vinícius de Matos Gomes, Marcelo Henrique Manfredo, Leandro Vaz Toffoli, Daniellen Christine Castro-Alves, Lucas Magnoni do Nascimento, Wyllian Rafael da Silva, Roberto Kiyoshi Kashimoto, Gelson Marcos Rodrigues-Jr, Viviane Batista Estrada, Rodrigo Antonio Andraus, Gislaine Garcia Pelosi

Published in: Lasers in Medical Science | Issue 7/2016

Abstract

The use of light emitting diodes (LED) as a therapeutic resource for wound healing has increased over the last years; however, little is still known about the molecular pathways associated to LED exposure. In the present study, we verified the effects of LED therapy on DNA methylation and expression of the DNA methyltransferase (Dnmt) genes, Dnmt1 and Dnmt3a, in an in vivo model of epithelial wound healing. Male Wistar rats were submitted to epithelial excision in the dorsal region and subsequently distributed within the experimental groups: group 1, animals that received irradiation of 0.8 J/cm² of LED (604 nm); group 2, animals that received 1.6 J/cm² of LED (604 nm); control (CTL), animals not submitted to therapeutic intervention. LED applications were performed during 7 days, and tissues from the periphery of the wound area were obtained for molecular analysis. The Image-J software was used for analysis of the wound area. DNA methylation was evaluated by ELISA-based method and gene expressions were quantified by real-time PCR. Decrease on global DNA methylation profile was observed in all experimental groups (CTL, 1, and 2) revealing the participation of DNA methylation in the healing process. Significant decrease in the wound area accompanied by increase in the Dnmt3a expression was associated to group 2. Based on our findings, we propose that DNA methylation is an important molecular mechanism associated to wound healing and that irradiation with 1.6 J/cm² of LED evokes an increase in the expression of the Dnmt3a that might associates to the efficiency of the epithelial wound healing.

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Title: Effects of the led therapy on the global DNA methylation and the expression of Dnmt1 and Dnmt3a genes in a rat model of skin wound healing
Authors: Marcus Vinícius de Matos Gomes
Marcelo Henrique Manfredo
Leandro Vaz Toffoli
Daniellen Christine Castro-Alves
Lucas Magnoni do Nascimento
Wyllian Rafael da Silva
Roberto Kiyoshi Kashimoto
Gelson Marcos Rodrigues-Jr
Viviane Batista Estrada
Rodrigo Antonio Andraus
Gislaine Garcia Pelosi
Publication date: 01-09-2016
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 7/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-016-2007-7

At a glance: The STEP trials

Springer Medicine

Effects of the led therapy on the global DNA methylation and the expression of Dnmt1 and Dnmt3a genes in a rat model of skin wound healing

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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