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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Effects of Shenlian extract on experimental atherosclerosis in ApoE-deficient mice based on ultrasound biomicroscopy

Authors: Yan Guo, Xu-Cen Liu, Ya-Jie Wang, Qi Li, Qing Yang, Xiao-Gang Weng, Ying Chen, Wei-Yan Cai, Xiao-Xi Kan, Xi Chen, He-Fei Huang, Xiao-Xin Zhu, Yu-Jie Li

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

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Abstract

Background

This study directly and dynamically investigated the effects of SL extract (i.e., a combination of Radix Salviae miltiorrhizae and Andrographis paniculata extract) on plaque progression in vivo by high resolution ultrasound biomicroscopy (UBM).

Methods

An atherosclerosis model was established by placing a perivascular collar on the right common carotid artery in apolipoprotein E-deficient (ApoE-/-) mice. Thickness, plaque area and local blood flow were observed by UBM, pathological changes were observed by histochemical staining, and lipid levels were measured by respective commercially available kits.

Results

Compared with the model group, the SL extract groups showed reduced wall thickness of the aortic arch (GC: P = 0.001, P = 0.002, and P < 0.001; LC: P < 0.001, P < 0.001, and P < 0.001; BC: P = 0.027, P = 0.017, and P = 0.003; respectively), which presented with retarded plaque progression of the cartoid artery with concordantly increased blood flow (P = 0.002 and P < 0.001) as visualized in vivo by UBM. Histological analysis confirmed the reduction of carotid atherosclerosis.

Conclusions

The SL extract inhibited the formation of atherosclerotic plaques in an ApoE-/- mice model by UBM analysis, and did so by effects that ameliorated local blood flow and improved blood lipid levels.
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Metadata
Title
Effects of Shenlian extract on experimental atherosclerosis in ApoE-deficient mice based on ultrasound biomicroscopy
Authors
Yan Guo
Xu-Cen Liu
Ya-Jie Wang
Qi Li
Qing Yang
Xiao-Gang Weng
Ying Chen
Wei-Yan Cai
Xiao-Xi Kan
Xi Chen
He-Fei Huang
Xiao-Xin Zhu
Yu-Jie Li
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1449-6

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