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Knee Surgery, Sports Traumatology, Arthroscopy

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01-02-2012 | Experimental Study

Effects of perfusion and cyclic compression on in vitro tissue engineered meniscus implants

Authors: M. Petri, K. Ufer, I. Toma, C. Becher, E. Liodakis, S. Brand, P. Haas, C. Liu, B. Richter, C. Haasper, G. von Lewinski, M. Jagodzinski

Published in: Knee Surgery, Sports Traumatology, Arthroscopy | Issue 2/2012

Abstract

Purpose

The purpose of this study was to investigate the influence of continuous perfusion and mechanical stimulation on bone marrow stromal cells seeded on a collagen meniscus implant.

Methods

Bone marrow aspirates from 6 donors were amplified in vitro. 10⁶ human BMSC were distributed on a collagen meniscus implant. Scaffolds were cultured under static conditions (control) or placed into a bioreactor system where continuous perfusion (10 ml/min) or perfusion and mechanical stimulation (8 h of 10% cyclic compression at 0.5 Hz) were administered daily. After 24 h, 7 and 14 days, cell proliferation, synthesis of procollagen I and III peptide (PIP, PIIIP), histology, and the equilibrium modulus of the constructs were analyzed.

Results

Proliferation demonstrated a significant increase over time in all groups (p < 0.001). PIP synthesis was found to increase from 0.1 ± 0.0 U/ml/g protein after 24 h to 2.0 ± 0.5 (perfusion), 3.8 ± 0.3 (mechanical stimulation), and 1.8 ± 0.2 U/ml/g protein (static control, lower than perfusion and mechanical stimulation, p < 0.05). These differences were also evident after 2 weeks (2.7 ± 0.3, 4.0 ± 0.6, and 1.8 ± 0.2 U/ml/g protein, p < 0.01); PIIIP synthesis was found to increase from 0.1 ± 0.0 U/ml/g protein after 24 h to 2.9 ± 0.7 (perfusion), 3.1 ± 0.9 (mechanical stimulation), and 1.6 ± 0.3 U/ml/g protein (controls) after 1 week and remained significantly elevated under the influence of perfusion and mechanical stimulation (p < 0.01) after 2 weeks. Mechanical stimulation increased the equilibrium modulus more than static culture and perfusion after 2 weeks (24.7 ± 7.6; 12.3 ± 3.7; 15.4 ± 2.6 kPa; p < 0.02).

Conclusion

Biomechanical stimulation and perfusion have impact on collagen scaffolds seeded with BMSCs. Cell proliferation can be enhanced using continuous perfusion and differentiation is fostered by mechanical stimulation.

Level of evidence

Non-applicable experimental study.

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Title: Effects of perfusion and cyclic compression on in vitro tissue engineered meniscus implants
Authors: M. Petri
K. Ufer
I. Toma
C. Becher
E. Liodakis
S. Brand
P. Haas
C. Liu
B. Richter
C. Haasper
G. von Lewinski
M. Jagodzinski
Publication date: 01-02-2012
Publisher: Springer-Verlag
Published in: Knee Surgery, Sports Traumatology, Arthroscopy / Issue 2/2012
Print ISSN: 0942-2056
Electronic ISSN: 1433-7347
DOI: https://doi.org/10.1007/s00167-011-1600-3

At a glance: The STEP trials

Springer Medicine

Effects of perfusion and cyclic compression on in vitro tissue engineered meniscus implants

Abstract

Purpose

Methods

Results

Conclusion

Level of evidence

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

Level of evidence

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