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Lasers in Medical Science
Published in: Lasers in Medical Science 5/2016

01-07-2016 | Original Article

Effects of low-level laser therapy combined with toluidine blue on polysaccharides and biofilm of Streptococcus mutans

Authors: S. S. de Sousa Farias, M. A. Nemezio, S. A. M. Corona, C. P. Aires, M. C. Borsatto

Published in: Lasers in Medical Science | Issue 5/2016

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Abstract

The aim of this study was to evaluate the effect of a low-level laser therapy in combination with toluidine blue on polysaccharides and biofilm of Streptococcus mutans. S. mutans biofilms were formed on acrylic resin blocks. These biofilms were exposed eight times/day to 10 % sucrose, and two times/day, they were subjected to one of the following treatments: G1, 0.9 % NaCl as a negative control; G2, 0.12 % chlorhexidine digluconate (CHX) as a positive antibacterial control; and G3 and G4 antimicrobial photodynamic therapy (aPDT) combined with toluidine blue using dosages of 320 and 640 J/cm2, respectively. The experiment was performed in triplicate. The biofilm formed on each block was collected for determination of the viable bacteria and concentration of insoluble extracellular polysaccharides (IEPS) and intracellular polysaccharides (IPS). CHX and aPDT treatments were able to inhibit bacterial growth in comparison with negative control (p < 0.05). The aPDT treatment reduced the number of viable bacteria formed in the S. mutans biofilm, in a dose-dependent manner (p < 0.05). The concentration of IEPS and IPS in the biofilms formed in presence of aPDT did not differ each other or in comparison to CHX (p > 0.05). The results suggest that low-level laser therapy presents effects on biofilm bacteria viability and in polysaccharides concentration.
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Metadata
Title
Effects of low-level laser therapy combined with toluidine blue on polysaccharides and biofilm of Streptococcus mutans
Authors
S. S. de Sousa Farias
M. A. Nemezio
S. A. M. Corona
C. P. Aires
M. C. Borsatto
Publication date
01-07-2016
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 5/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-016-1944-5

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