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BMC Complementary Medicine and Therapies

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Open Access 01-12-2015 | Research article

Effects of kolaviron on hepatic oxidative stress in streptozotocin induced diabetes

Authors: Omolola R. Oyenihi, Nicole L. Brooks, Oluwafemi O. Oguntibeju

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

Abstract

Background

Alteration in antioxidant defence and increase in oxidative stress that results in tissue injury is characteristic of diabetes. We evaluated the protective effects of kolaviron (a flavonoid complex extracted from the seeds of Garcinia kola) on hepatic antioxidants, lipid peroxidation and apoptosis in diabetic rats.

Methods

To induce diabetes, rats were injected with streptozotocin intraperitoneally at a single dose of 50 mg/kg. Kolaviron (100 mg/kg) was administered orally for 6 weeks (5 times weekly). Activities of liver antioxidant enzymes was analysed with Multiskan Spectrum plate reader. High performance liquid chromatography (HPLC) was used in the analysis of MDA (malondialdehyde), a product of lipid peroxidation. Apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay.

Result

Diabetic rats exhibited a significant increase in the peroxidation of hepatic lipids as observed from the elevated level of malondialdehyde (MDA). In addition, Oxygen Radical Absorbance Capacity (ORAC), level of reduced glutathione (GSH), ratio of reduced to oxidized glutathione (GSH: GSSG) and catalase (CAT) activity were decreased in the liver of diabetic rats. The activities of GPX (glutathione peroxidase) and SOD (superoxide dismutase) were unaltered in diabetic rats. TUNEL assay revealed increased apoptotic cell death in the liver. Kolaviron attenuated lipid peroxidation and apoptosis, increased CAT activity, GSH levels and GSH: GSSG ratio. The ORAC of kolaviron-treated diabetic liver was restored to near-normal values.

Conclusion

Kolaviron protects the liver against oxidative and apoptotic damage induced by hyperglycemia.

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Title: Effects of kolaviron on hepatic oxidative stress in streptozotocin induced diabetes
Authors: Omolola R. Oyenihi
Nicole L. Brooks
Oluwafemi O. Oguntibeju
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-015-0760-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of kolaviron on hepatic oxidative stress in streptozotocin induced diabetes

Abstract

Background

Methods

Result

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Result

Conclusion

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