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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2015

Open Access 01-12-2015 | Research article

Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca2+ and ERK1/ERK2 signaling

Authors: Abdelhafid Nani, Meriem Belarbi, Wided Ksouri-Megdiche, Souleymane Abdoul-Azize, Chahid Benammar, François Ghiringhelli, Aziz Hichami, Naim Akhtar Khan

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

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Abstract

Background

Pearl millet (PM), i.e., Pennisetum glaucum, is widely grown in Africa and known for its anti-oxidant and anti-hyperlipidemic properties.

Methods

The P. glaucumgrains were obtained from the region of Ouled Aïssa (South of Algeria).  We assessed the effects of phenolic compounds and lipids, extracted from seeds of P. glaucum, on rat lymphocyte proliferation, activated by phorbol 12-myristate 13-acetate and ionomycin. In order to explore signaling pathway, triggered by these compounds, we assessed interleukin-2 (IL-2) mRNA expression and extracellular signal-regulated kinase-1/2 (ERK1/ERK2) phosphorylation. Finally, we determined increases in free intracellular Ca2+ concentrations, [Ca2+]i, by employing Fura-2/AM in rat lymphocytes.

Results

The composition of P. glaucum grains in polyphenols was estimated to be 1660 µg gallic acid equivalents (GAE)/g. Lipids represented 4.5 %, and more than 72% of the fatty acids belonged to unsaturated family. Our investigation showed that both lipid and phenolic compounds inhibited mitogen-induced T-cell proliferation. Compared with phenolic compounds, lipids exerted weaker effects on ERK-1/ERK2 phosphorylation and Ca2+ signaling in mitogen-activated T-cells.

Conclusion

We conclude that the immunomodulatory effects of P. glaucum could be contributed by its phenolic and lipid contents.
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Metadata
Title
Effects of polyphenols and lipids from Pennisetum glaucum grains on T-cell activation: modulation of Ca2+ and ERK1/ERK2 signaling
Authors
Abdelhafid Nani
Meriem Belarbi
Wided Ksouri-Megdiche
Souleymane Abdoul-Azize
Chahid Benammar
François Ghiringhelli
Aziz Hichami
Naim Akhtar Khan
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-015-0946-3

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