Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Lasers in Medical Science
Published in: Lasers in Medical Science 2/2015

01-02-2015 | Original Article

Effects of increased low-level diode laser irradiation time on extraction socket healing in rats

Authors: Joon Bong Park, Su-Jin Ahn, Yoon-Goo Kang, Eun-Cheol Kim, Jung Sun Heo, Kyung Lhi Kang

Published in: Lasers in Medical Science | Issue 2/2015

Login to get access

Abstract

In our previous studies, we confirmed that low-level laser therapy (LLLT) with a 980-nm gallium–aluminum–arsenide diode laser was beneficial for the healing of the alveolar bone in rats with systemic disease. However, many factors can affect the biostimulatory effects of LLLT. Thus, we attempted to investigate the effects of irradiation time on the healing of extraction sockets by evaluating the expressions of genes and proteins related to bone healing. The left and right first maxillary molars of 24 rats were extracted. Rats were randomly divided into four groups in which extraction sockets were irradiated for 0, 1, 2, or 5 min each day for 3 or 7 days. Specimens containing the sockets were examined using quantitative real-time reverse transcription polymerase chain reaction and western blotting. LLLT increased the expressions of all tested genes, Runx2, collagen type 1, osteocalcin, platelet-derived growth factor-B, and vascular endothelial growth factor, in a time-dependent manner. The highest levels of gene expressions were in the 5-min group after 7 days. Five minutes of irradiation caused prominent increases of the expression of all tested proteins after both 3 and 7 days. The expression level of each protein in group 4 was higher by almost twofold compared with group 1 after 7 days. Laser irradiation for 5 min caused the highest expressions of genes and proteins related to bone healing. In conclusion, LLLT had positive effects on the early stages of bone healing of extraction sockets in rats, which were irradiation time-dependent.
Literature
1.
Cardaropoli G, Araujo M, Lindhe J (2003) Dynamics of bone tissue formation in tooth extraction sites. An experimental study in dogs. J Clin Periodontol 30:809–818PubMedCrossRef
2.
Berglundh T, Abrahamsson I, Lang NP, Lindhe J (2003) De novo alveolar bone formation adjacent to endosseous implants. Clin Oral Implants Res 14:251–262PubMedCrossRef
3.
Villars F, Bordenave L, Bareille R, Amédée J (2000) Effect of human endothelial cells on human bone marrow stromal cell phenotype: role of VEGF? J Cell Biochem 79:672–685PubMedCrossRef
4.
5.
6.
Lubart R, Lavi R, Friedmann H, Rochkind S (2006) Photochemistry and photobiology of light absorption by living cell. Photomed Laser Surg 24:179–185PubMedCrossRef
7.
Jakse N, Payer M, Tangl S, Berghold A, Kirmeier R, Lorenzoni M (2007) Influence of low-level laser treatment on bone regeneration and osseointegration of dental implants following sinus augmentation. An experimental study on sheep. Clin Oral Implants Res 18:517–524PubMedCrossRef
8.
Jahangiri Noudeh Y, Shabani M, Vatankhah N, Hashemian SJ, Akbari K (2010) A combination of 670 nm and 810 nm diode lasers for wound healing acceleration in diabetic rats. Photomed Laser Surg 28:621–627PubMedCrossRef
9.
Aykol G, Baser U, Maden I, Kazak Z, Onan U, Tanrikulu-Kucuk S, Ademoglu E, Issever H, Yalcin F (2011) The effect of low-level laser therapy as an adjunct to non-surgical periodontal treatment. J Periodontol 82:481–488PubMedCrossRef
10.
Li L, Zhu Z, Huang C, Chen W (2008) Ultrasound: a potential technique to improve osseointegration of dental implants. Med Hypotheses 71:568–571PubMedCrossRef
11.
Fini M, Giavaresi G, Giardino R, Cavani F, Cadossi R (2006) Histomorphometric and mechanical analysis of the hydroxyapatite-bone interface after electromagnetic stimulation: an experimental study in rabbits. J Bone Joint Surg Br 88:123–128PubMedCrossRef
12.
Fahimipour F, Mahdian M, Houshmand B, Asnaashari M, Sadrabadi AN, Farashah SE, Mousavifard SM, Khojasteh A (2013) The effect of He-Ne and Ga-Al-As laser light on the healing of hard palate mucosa of mice. Lasers Med Sci 28:93–100PubMedCrossRef
13.
Lopes CB, Pinheiro AL, Sathaia S, Duarte J, Cristinamartins M (2005) Infrared laser light reduces loading time of dental implants: a raman spectroscopic study. Photomed Laser Surg 23:27–31PubMedCrossRef
14.
Pretel H, Lizarelli RF, Ramalho LT (2007) Effect of low-level laser therapy on bone repair: histological study in rats. Lasers Surg Med 39:788–796PubMedCrossRef
15.
Vladimirov YA, Osipov AN, Klebanov GI (2004) Photobiological principles of therapeutic applications of laser radiation. Biochemistry (Mosc) 69:81–90CrossRef
16.
Kang KL, Chung JH (2009) Effect of low-energy laser irradiation on healing of extraction sockets in ovariectomized Rats. Tissue Eng Regen 6:1310–1320
17.
Park JJ, Kang KL (2012) Effect of 980-nm GaAlAs diode laser irradiation on healing of extraction sockets in streptozotocin-induced diabetic rats: a pilot study. Lasers Med Sci 27:223–230PubMedCrossRef
18.
Lomke MA (2009) Clinical applications of dental lasers. Gen Dent 57:47–59PubMed
19.
Silveira PC, Streck EL, Pinho RA (2007) Evaluation of mitochondrial repiratory chain activity in wound healing by low-level laser therapy. J Photochem Photobiol B 86:279–282PubMedCrossRef
20.
Alexandratou E, Yova D, Handris P, Kletsas D, Loukas S (2002) Human fibroblast alterations induced by low power laser irradiation at the sing cell level using confocal microscopy. Photochem Photobiol Sci 1:547–552PubMedCrossRef
21.
Hawkins D, Abrahamse H (2006) Effect of multiple exposures of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. Photomed Laser Surg 24:705–714PubMedCrossRef
22.
Lin Z, Rios HF, Volk SL, Sugai JV, Jin Q, Giannobile WV (2011) Gene expression dynamics during bone healing and osseointegration. J Periodontol 82:1007–1017PubMedCentralPubMedCrossRef
23.
Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G (1997) Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell 89:747–754PubMedCrossRef
24.
Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T (1997) Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturation arrest of osteoblasts. Cell 89:755–764PubMedCrossRef
25.
Min HJ, Lee MJ, Kim JY, Cho SW, Park HD, Lee SI, Kim HJ, Jung HS (2007) Alteration of BMP-4 and Runx2 expression patterns in mouse temporomandibular joint after ovarectomy. Oral Dis 13:220–227PubMedCrossRef
26.
Korany NS, Mehanni SS, Hakam HM, El-Maghraby EM (2012) Evaluation of socket healing in irradiated rats after diode laser exposure (histological and morphometric studies). Arch Oral Biol 57:884–891PubMedCrossRef
27.
Hughes FJ, Turner W, Belibasakis G et al (2006) Effects of growth factors and cytokines on osteoblast differentiation. Periodontol 2000 41:48–72PubMedCrossRef
28.
Vermeulen AH, Vermeer C, Bosman FT (1989) Histochemical detection of osteocalcin in normal and pathological human bone. J Histochem Cytochem 37:1503–1508PubMedCrossRef
29.
Tanaka S, Matsuzaka K, Sato D, Inoue T (2007) Characteristics of newly formed bone during guided bone regeneration: analysis of Cbfa-1, osteocalcin, and VEGF expression. J Oral Implantol 33:321–326PubMedCrossRef
30.
Götz W, Gerber T, Michel B, Lossdörfer S, Henkel KO, Heinemann F (2008) Immunohistochemical characterization of nanocrystalline hydroxiapatite silica gel (NanoBone(s)) osteogenesis: a study on biopsies from human jaws. Clin Oral Implants Res 19:1016–1026PubMedCrossRef
31.
Li G, Cui Y, McIlmurray L, Allen WE, Wang H (2005) rhBMP-2, rhVEGF(165), rhPTN and thrombin-related peptide, TP508 induce chemotaxis of human osteoblasts and microvascular endothelial cells. J Orthop Res 23:680–685PubMedCrossRef
32.
Deckers MM, van Bezooijen RL, van der Horst G, Hoogendam J, van Der Bent C, Papapoulos SE, Löwik CW (2002) Bone morphogenetic proteins stimulate angiogenesis through osteoblast-derived vascular endothelial growth factor A. Endocrinology 143:1545–1553PubMedCrossRef
33.
Wang DS, Miura M, Demura H, Sato K (1997) Anabolic effects of 1,25-dihydroxyvitamin D3 on osteoblasts are enhanced by vascular endothelial growth factor produced by osteoblasts and by growth factors produced by endothelial cells. Endocrinology 138:2953–2962PubMed
34.
Bouletreau PJ, Warren SM, Spector JA, Peled ZM, Gerrets RP, Greenwald JA, Longaker MT (2002) Hypoxia and VEGF up-regulate BMP-2 mRNA and protein expression in microvascular endothelial cells: implications for fracture healing. Plast Reconstr Surg 109:2384–2397PubMedCrossRef
35.
Feng J, Zhang Y, Xing D (2012) Low-power laser irradiation (LPLI) promotes VEGF expression and vascular endothelial cell proliferation through the activation of ERK/Sp1 pathway. Cell Signal 24:1116–1125PubMedCrossRef
36.
Hughes FJ, McCulloch CA (1991) Quantification of chemotactic response of quiescent and proliferating fibroblasts in Boyden chambers by computer-assisted image analysis. J Histochem Cytochem 39:243–246PubMedCrossRef
37.
Hughes FJ, Aubin JE, Heersche JN (1992) Differential chemotatic responses of different populations of fetal rat calvaria cells to platelet-derived growth factor and transforming growth factor β. Bone Miner 19:63–74PubMedCrossRef
38.
Anusaksathien O, Jin Q, Zhao M, Somerman MJ, Giannobile WV (2004) Effect of sustained gene delivery of platelet-derived growth factor or its antagonist (PDGF-1308) on tissue-engineered cementum. J Periodontol 75(3):429–440PubMedCentralPubMedCrossRef
39.
Canalis E, McCarthy TL, Centrella M (1989) Effects of platelet-derived growth factor on bone formation in vitro. J Cell Physiol 140:530–537PubMedCrossRef
40.
Hollinger JO, Hart CE, Hirsch SN, Lynch S, Friedlaender GE (2008) Recombinant human platelet-derived growth factor: biology and clinical applications. J Bone Joint Surg Am 90:48–54PubMedCrossRef
41.
Barnes GL, Kostenuik PJ, Gerstenfeld LC, Einhorn TA (1999) Growth factor regulation of fracture repair. J Bone Miner Res 14:1805–1815PubMedCrossRef
42.
Hart CE, Bailey M, Curtis DA (1990) Purification of PDGF-AB and PDGF-BB from human platelet extracts and identification of all three PDGF dimers in human platelets. Biochemistry 29:166–172PubMedCrossRef
43.
Heldin CH, Westermark B (1999) Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev 79:1283–1316PubMed
44.
Chang PC, Seol YJ, Cirelli JA, Pellegrini G, Jin Q, Franco LM, Goldstein SA, Chandler LA, Sosnowski B, Giannobile WV (2010) PDGF-B gene therapy accelerates bone engineering and oral implant osseointegration. Gene Ther 17:95–104PubMedCentralPubMedCrossRef
45.
Saito S, Shimizu N (1997) Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod Dentofac Orthop 111:525–532CrossRef
46.
Lomke MA (2009) Clinical applications of dental lasers. Gen Dent 57:47–59PubMed
47.
Chung TY, Peplow PV, Baxter GD (2010) Laser photobiostimulation of wound healing: defining a dose response for splinted wounds in diabetic mice. Lasers Surg Med 42:656–664PubMedCrossRef
48.
Al-Watban FA, Andres BL (2012) Laser biomodulation of normal and neoplastic cells. Lasers Med Sci 27:1039–1043PubMedCrossRef
49.
50.
Bossini PS, Rennó AC, Ribeiro DA, Fangel R, Ribeiro AC, Lahoz Mde A, Parizotto NA (2012) Low level laser therapy (830nm) improves bone repair in osteoporotic rats: similar outcomes at two different dosages. Exp Gerontol 47:136–142PubMedCrossRef
Metadata
Title
Effects of increased low-level diode laser irradiation time on extraction socket healing in rats
Authors
Joon Bong Park
Su-Jin Ahn
Yoon-Goo Kang
Eun-Cheol Kim
Jung Sun Heo
Kyung Lhi Kang
Publication date
01-02-2015
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 2/2015
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-013-1402-6

Other articles of this Issue 2/2015

Lasers in Medical Science 2/2015 Go to the issue

Original Article

Significance of scaling and root planing with and without adjunctive use of a water-cooled pulsed Nd:YAG laser for the treatment of periodontal inflammation

Original Article

Effects of low-level laser therapy on orthodontics: rate of tooth movement, pain, and release of RANKL and OPG in GCF

Original Article

In vitro evaluation of enamel demineralization after several overlapping CO2 laser applications

Original Article

Human papilloma virus lesions of the oral cavity: healing and relapse after treatment with 810–980 nm diode laser

Original Article

Efficacy of laser-based irrigant activation methods in removing debris from simulated root canal irregularities

Letter to the Editor

Reply to comments on: “Efficacy of low-level laser therapy in the management of orthodontic pain: a systematic review and meta-analysis”