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Journal of Gastroenterology
Published in: Journal of Gastroenterology 8/2017

01-08-2017 | Original Article-Alimentary Tract

Effects of estrogen on esophageal function through regulation of Ca2+-related proteins

Authors: Kipung Kim, Dongoh Lee, Changhwan Ahn, Hee Young Kang, Beum-Soo An, Yeon Hee Seong, Eui-Bae Jeung

Published in: Journal of Gastroenterology | Issue 8/2017

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Abstract

Background

The calcium ion is important for physiological functions in all tissues and organs and essential to many vital functions, including hormone secretion and muscle contraction. The intracellular concentration of calcium is regulated by calcium related proteins such as CaBP-9k, PMCA1, and NCX1. In this study, we investigated the relationship between calcium regulation and esophageal functions such as mucin secretion and smooth muscle contraction.

Methods

To evaluate the influence of sex steroid hormones, immature rats were treated for 3 days with estradiol (E2), progesterone (P4), and their antagonists (ICI 182,780, and RU486). Esophageal function, transcription level, and localization of CaBP-9k, PMCA1, NCX1, ERα, and MUC2 were examined in the esophagus.

Results

Transcriptional level of Cabp-9k and Muc2 was increased by E2, but not by P4. CaBP-9k, PMCA1, and MUC2 were mainly localized in the mucosal layer. Acidic mucosubstances in the esophagus were increased by E2 and recovered by ICI treatment. Unlike the expression of Cabp-9k, mRNA levels of Pmca1, Ncx1, and Erα were only decreased in response to E2, and recovered by ICI co-treatment group. The contraction of the esophagus and mRNA level of Mylk were reduced by E2. Overall, E2 upregulated mucus secretion, but downregulated muscle contraction in the esophagus through regulation of the expression of calcium related genes and the resultant intracellular calcium level.

Conclusions

The regulation of E2 in the function of esophagus may be applied to treat esophageal diseases such as reflux esophagitis, achalasia, and esophageal cancer.
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Metadata
Title
Effects of estrogen on esophageal function through regulation of Ca2+-related proteins
Authors
Kipung Kim
Dongoh Lee
Changhwan Ahn
Hee Young Kang
Beum-Soo An
Yeon Hee Seong
Eui-Bae Jeung
Publication date
01-08-2017
Publisher
Springer Japan
Published in
Journal of Gastroenterology / Issue 8/2017
Print ISSN: 0944-1174
Electronic ISSN: 1435-5922
DOI
https://doi.org/10.1007/s00535-016-1305-y

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