Top

Published in:

01-06-2013

Effects of Canavalia lectins on Acute Inflammation in Sensitized and Non-sensitized Rats

Authors: Nilson Vieira Pinto, Benildo Sousa Cavada, Lucas Ferreira Brito, Ronniery Ilario Pereira, Mayara Torquato Lima da Silva, Rondinelle Ribeiro Castro, Alana de Freitas Pires, Ana Maria Sampaio Assreuy

Published in: Inflammation | Issue 3/2013

Abstract

The anti-inflammatory activity of Canavalia seed lectins (Canavalia gladiata [CGL], Canavalia maritima [ConM] and Canavalia brasiliensis [ConBr]) was evaluated by intravenous administration in rats. In non-sensitized rats, cellular edema elicited by carrageenan was reduced (45–51 %) by ConM and (44–59 %) by CGL. Osmotic edema elicited by dextran was reduced by ConM and CGL in 27 % and 29 %. ConM and CGL reduced the edema elicited by l-arginine in 53 % and that of prostaglandin E₂ in 48 % and 36 %. Leukocyte migration elicited by carrageenan was reduced in 49 % by ConM and in 55 % by CGL (attenuated in 4× by glucose) and peritoneal TNF-α content in 82 %. In rats sensitized, ConM inhibited the paw edema and leukocyte migration elicited by ovalbumin in 34 % and 70 %. ConM and CGL are anti-inflammatory, mainly in cellular events mediated by prostaglandin E₂, nitric oxide and TNF-α in non-sensitized rats. However, only ConM is anti-inflammatory in sensitized rats. CGL effect involves the lectin domain.

Feitosa, R.F., G.B. Melcíades, A.M. Assreuy, M.F. Rocha, R.A. Ribeiro, and A.A. Lima. 2002. The pharmacological profile of ovalbumin-induced paw oedema in rats. Mediators of Inflammation 11: 155–163.PubMedCrossRef

Peumans, W.J., and E.J.M. Van Damme. 1995. Lectins as plant defense proteins. Plant Physiology 109: 347–352.PubMedCrossRef

Loris, R., T. Hamelryck, J. Bouckaert, and L. Wyns. 1998. Legume lectin structure. Biochemistry Biophysics Acta 1383: 9–36.CrossRef

Bezerra, G.A., T.M. Oliveira, F.B. Moreno, E.P. de Souza, B.A. da Rocha, R.G. Benevides, P. Delatorre, W.F. de Azevedo, and B.S. Cavada JR. 2007. Structural analysis of Canavalia maritima and Canavalia gladiata lectins complexed with different dimannosides: new insights into the understanding of the structure–biological activity relationship in legume lectins. Journal of Structural Biology 160: 168–176.

Delatorre, P., B.A.M. Rocha, C.A.A. Gadelha, T. Santi-Gadelha, J.B. Cajazeiras, E.P. Souza, and K.S. Nascimento. 2006. Crystal structure of a lectin from Canavalia maritima (ConM) in complex with trehalose and maltose reveals relevant mutation in ConA-like lectins. Journal of Structural Biology 154: 280–286.PubMedCrossRef

Bento, C.A.M., B.S. Cavada, J.T.A. Oliveira, R.A. Moreira, and C. Barja-Fidalgo. 1993. Rat paw edema and leukocyte emigration induced by plant lectins. Agents and Actions 38: 48–54.PubMedCrossRef

Figueiredo, J.G., B.F. Silveira, M.R. Mota, P.P. Silvestre, C.N. Aguiar, R.G. Benevides, K.S. Nascimento, T.R. Moura, D. Dal-Secco, A.M. Assreuy, F.Q. Cunha, M.R. Vale, B.S. Cavada, and N.M. Alencar. 2009. Pharmacological analysis of the neutrophil migration induced by D. rostrata lectin: Involvement of cytokines and nitric oxide. Toxicon 54: 736–744.PubMedCrossRef

Rangel, T.B.A., A.M.S. Assreuy, A.F. Pires, A.U. Carvalho, R.G. Benevides, R.C. Simões, H.C. Silva, M.J.B. Bezerra, A.S.F. Nascimento, K.S. Nascimento, C.S. Nagano, A.H. Sampaio, P. Delatorre, B.A.M. Rocha, P.M.B. Fernandes, and B.S. Cavada. 2011. Crystallization and characterization of an inflammatory lectin purified from the seeds of Dioclea wilsonii. Molecules 16: 5087–5103.PubMedCrossRef

Rangel, T.B., B.A. Rocha, G.A. Bezerra, A.M. Assreuy, A.F. Pires, A.S. do Nascimento, M.J. Bezerra, K.S. do Nascimento, C.S. Nagano, A.H. Sampaio, K. Gruber, P. Delatorre, P.M. Fernandes, and B.S. Cavada. 2012. Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii Standl. Biochimie 94: 525–532.PubMedCrossRef

10.

Delatorre, P., B.A. Rocha, R.C. Simões, F.N. Pereira-Júnior, H.C. Silva, E.H. Bezerra, M.J. Bezerra, E.S. Marinho, C.A. Gadelha, T. Santi-Gadelha, D.L. Farias, A.M.S. Assreuy, G.F.O. Marques-Domingos, C.S. Nagano, and B.S. Cavada. 2011. Mass spectrometry and X-ray diffraction analysis of two crystal types of Dioclea virgata lectin: An antinociceptive protein candidate to structure/function analysis. Applied Biochemistry and Biotechnology 164: 741–754.PubMedCrossRef

11.

Assreuy, A.M.S., S.R. Fontenele, A.F. Pires, D.C. Fernandes, N.V.F.C. Rodrigues, E.H.S. Bezerra, T.R. Moura, K.S. Nascimento, and B.S. Cavada. 2009. Vasodilator effects of Diocleinae lectins from the Canavalia genus. N-S Arch Pharmacology 380: 509–521.CrossRef

12.

Simões, R.C., B.A. Rocha, M.J. Bezerra, I.L. Barroso-Neto, F.N. Pereira-Junior, R. da Mata Moura, K.S. do Nascimento, C.S. Nagano, P. Delatorre, A.F. Pires, A.M. Assreuy, A.H. Sampaio, and B.S. Cavada. 2012. Protein crystal content analysis by mass spectrometry and preliminary X-ray diffraction of a lectin from Canavalia grandiflora seeds with modulatory role in inflammation. Rapid Communications in Mass Spectrometry 26: 811–818.PubMedCrossRef

13.

Rocha, B.A., P. Delatorre, T.M. Oliveira, R.G. Benevides, A.F. Pires, A.A. Sousa, L.A. Souza, A.M. Assreuy, H. Debray, W.F. Azevedo Junior, A.H. Sampaio, and B.S. Cavada. 2011. Structural basis for both pro- and anti-inflammatory response induced by mannose-specific legume lectin from Cymbosema roseum. Biochimie 9: 806–816.CrossRef

14.

Assreuy, A.M.S., M.D. Shibuya, G.J. Martins, M.L.P. Souza, B.S. Cavada, R.A. Moreira, J.T.A. Oliveira, R.A. Ribeiro, and C.A. Flores. 1997. Anti-inflammatory effect of glucose–mannose binding lectins isolated from Brazilian beans. Mediators of Inflammation 6: 201–210.PubMedCrossRef

15.

Assreuy, A.M.S., G.J. Martins, M.E.F. Moreira, G.A. Brito, B.S. Cavada, R.A. Ribeiro, and C.A. Flores. 1999. Prevention of cyclophosphamide-induced hemorrhagic cystitis by glucose–mannose binding plant lectins. Journal of Urology 161: 1988–1993.PubMedCrossRef

16.

Alencar, N.M.N., E.H. Teixeira, A.M.S. Assreuy, B.S. Cavada, C.A. Flores, and R.A. Ribeiro. 1999. Leguminous lectins as tools for studying the role of sugar residues in leukocyte recruitment. Mediators of Inflammation 8: 107–113.PubMedCrossRef

17.

Nunes, B.S., N.S. Rensonnet, D. Dal-Secco, S.M. Vieira, B.S. Cavada, E.H. Teixeira, T.R. Moura, C.S. Teixeira, J.T. Clemente-Napimoga, F.Q. Cunha, and M.H. Napimoga. 2009. Lectin extracted from Canavalia grandiflora seeds presents potential anti-inflammatory and analgesic effects. N-S Arch Pharmacology 379: 609–616.CrossRef

18.

Napimoga, M.H., B.S. Cavada, N.M. Alencar, M.L. Mota, F.S. Bittencourt, J.C. Alves-Filho, R. Grespan, R.B. Gonçalves, J.T. Clemente-Napimoga, C.A. Parada, S.H. Ferreira, and F.Q. Cunha. 2007. Lonchocarpus sericeus lectin decreases leukocyte migration and mechanical hypernociception by inhibiting cytokine and chemokines production. International Immunopharmacology 7: 824–835.PubMedCrossRef

19.

Moreira, R.A., and B.S. Cavada. 1984. Lectin from Canavalia brasiliensis (MART). Isolation characterization and behavior during germination. Biologia Plantarum (Praha) 26: 113–120.CrossRef

20.

Moreno, F.B., P. Delatorre, B.T. Freitas, B.A. Rocha, E.P. Souza, F. Facó, F. Canduri, A.L. Cardoso, V.N. Freire, J.L. Lima Filho, A.H. Sampaio, J.J. Calvete, W.F. de Azevedo Jr., and B.S. Cavada. 2004. Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seeds. Acta Crystallographica Section D: Biological Crystallography 60: 1493–1495.

21.

Perez, G., C. Perez, B.S. Cavada, R. Moreira, and M. Richardson. 1991. Comparison of the amino acid sequences of the lectins from seeds of Dioclea lehmanni and Canavalia maritima. Phytochemistry 30: 2619–2621.PubMedCrossRef

22.

Landucci, E.C.T., E. Antunes, J.L. Donato, R. Faro, S. Hyslop, and S. Marangoni. 1995. Inhibition of carrageenin-induced rat paw edema by crotapotin a polypeptide complexed with phospholipase A2. British Journal Pharmacology Oxford 114: 578–583.CrossRef

23.

Souza, G.E.P., and S.H. Ferreira. 1985. Blockade by antimacrophage serum of the migration of PMN neutrophils into the inflamed peritoneal cavity. Agents and Actions 17: 97–103.PubMedCrossRef

24.

DiRosa, M., J.P. Giroud, and D.A. Willoughby. 1971. Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. Journal Path 104: 15–29.CrossRef

25.

Lo, T.N., A.P. Almeida, and M.A. Beaven. 1982. Dextran and carrageenan evoke different inflammatory responses in rat with respect to composition of infiltrates and effect of indomethacin. Journal of Pharmacology and Experimental Therapeutics 221: 261–267.PubMed

26.

Salvemini, D., Z.Q. Wang, P.S. Wyatt, D.M. Bourdon, M.H. Marino, P.T. Manning, and M.G. Currie. 1996. Nitric oxide: A key mediator in the early and late phase of carrageenan-induced rat paw inflammation. British Journal of Pharmacology 118: 829–838.PubMedCrossRef

27.

Vircheva, S., A. Alexandrova, A. Georgieva, P. Mateeva, R. Zamfirova, M. Kubera, and M. Kirkova. 2010. In vivo effects of pentoxifylline on enzyme and non-enzyme antioxidant levels in rat liver after carrageenan-induced paw inflammation. Cell Biochemistry and Function 28: 668–672.PubMedCrossRef

28.

Jahnova, E., A. Horvathova, and F.M. Gazdik. 1998. Expression of adhesion molecules and effect of disodium cromoglycate treatment in asthmatics. Physiological Research 47: 439–443.PubMed

29.

Klein, A., F.Q. Cunha, and S.H. Ferreira. 1995. The role of lymphocytes in the neutrophil migration induced by ovalbumin in immunised rats. Immunology 84: 577–584.PubMed

30.

Sharma, V., and A. Surolia. 1997. Analyses of carbohydrate recognition by legume lectins: size of the combining site loops and their primary specificity. Journal of Molecular Biology 267: 433–45.PubMedCrossRef

31.

Hamelryck, T.W., J.G. Moore, M.J. Chrispeels, R. Loris, and L. Wyns. 2000. The role of weak protein-protein interactions in multivalent lectin–carbohydrate binding: Crystal structure of cross-linked FRIL. Journal of Molecular Biology 16: 875–883.CrossRef

32.

Delatorre, P., B.A.M. Rocha, E.P. Souza, T.M. Oliveira, G.A. Bezerra, F.B. Moreno, and B.T. Freitas. 2007. Structure of a lectin from Canavalia gladiata seeds: new structural insights for old molecules. BMC Structural Biology 7: 52–60.PubMedCrossRef

33.

Campbell, P.A. 1990. The neutrophil a professional killer of bacteria may be controlled by T cells. Clinical and Experimental Immunology 79: 141–143.PubMedCrossRef

34.

Hilaly, J.E., Z.H. Israili, and B. Lyouss. 2004. Acute and chronic toxicological studies of Ajuva iva in experimental animals. Journal of Ethnopharmacology 91: 43–50.PubMedCrossRef

35.

Mukinda, J.T., and J.A. Syce. 2007. Acute and chronic toxicity of the aqueous extract of Artemisia afrainrodents. Journal of Ethnopharmacology 112: 138–144.PubMedCrossRef

Title: Effects of Canavalia lectins on Acute Inflammation in Sensitized and Non-sensitized Rats
Authors: Nilson Vieira Pinto
Benildo Sousa Cavada
Lucas Ferreira Brito
Ronniery Ilario Pereira
Mayara Torquato Lima da Silva
Rondinelle Ribeiro Castro
Alana de Freitas Pires
Ana Maria Sampaio Assreuy
Publication date: 01-06-2013
Publisher: Springer US
Published in: Inflammation / Issue 3/2013
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-013-9596-0

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Effects of Canavalia lectins on Acute Inflammation in Sensitized and Non-sensitized Rats

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2013

Effects of Chronic Exercise Training on Inflammatory Markers in Australian Overweight and Obese Individuals in a Randomized Controlled Trial

Oligopeptidases B from Trypanossoma cruzi and Trypanossoma brucei Inhibit Inflammatory Pain in Mice by Targeting Serotoninergic Receptors

Antiapoptotic and Proapoptotic Signaling of Cyclophilin A in Endothelial Cells

Taurine Enhances Antinociception Produced by a COX-2 Inhibitor in an Inflammatory Pain Model

Modulation of B Cell Regulatory Molecules CD22 and CD72 in Myasthenia Gravis and Multiple Sclerosis

Zinc Sulfate Inhibited Inflammation of Der p2-Induced Airway Smooth Muscle Cells by Suppressing ERK1/2 and NF-κB Phosphorylation

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page