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Published in: Odontology 2/2019

01-04-2019 | Original Article

Effectiveness of irrigation strategies on the removal of the smear layer from root canal dentin

Authors: Prasanna Neelakantan, Hani F. Ounsi, Sharmila Devaraj, Gary S. P. Cheung, Simone Grandini

Published in: Odontology | Issue 2/2019

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Abstract

The aim of this study was to evaluate the removal of the smear layer by some commonly used (needle-and-syringe irrigation, sonic activation, ultrasonically activated irrigation) and new root canal irrigation strategies (negative pressure irrigation and polymer rotary file) using a novel approach by comparing pre- and post-experimental images. Prepared root canals (n = 50) were subjected to a split tooth model and divided into 5 groups (n = 10): (1) needle-and-syringe irrigation (control); (2) sonic activation (SA); (3) negative pressure irrigation with continuous warm activated irrigation and evacuation (CWA); (4) polymer finishing file (FF); (5) ultrasonically activated irrigation (UAI). Smear layer scores and percentage of open dentinal tubules (%ODT) were evaluated by 2 examiners before and after irrigation procedures, from the middle and apical thirds of the root canal, on scanning electron microscopic images. Data were analysed using Kruskal–Wallis and post hoc tests at P = 0.05. Needle-and-syringe irrigation (control) showed no significant difference (both smear score and %ODT) compared to the pre-experimental value (P > 0.05). All other groups showed lower smear scores and higher %ODT, compared to the control (P < 0.05). The lowest smear score and highest %ODT were observed in the CWA group, which was significantly different from all other groups (P < 0.05). SA group showed significantly higher smear scores and lower %ODT than FF or UAI (P < 0.05). CWA showed superior removal of smear layer in the middle and apical thirds of the root canal compared to the other irrigation strategies.
Literature
1.
go back to reference Wang Z, Shen Y, Haapasalo M. Effect of smear layer against disinfection protocols on Enterococcus faecalis-infected dentin. J Endod. 2013;39:1395–400.CrossRefPubMed Wang Z, Shen Y, Haapasalo M. Effect of smear layer against disinfection protocols on Enterococcus faecalis-infected dentin. J Endod. 2013;39:1395–400.CrossRefPubMed
2.
3.
go back to reference Carvalho AS, Camargo CH, Valera MC, Camargo SE, Mancini MN. Smear layer removal by auxiliary chemical substances in biomechanical preparation: a scanning electron microscope study. J Endod. 2008;34:1396–400.CrossRefPubMed Carvalho AS, Camargo CH, Valera MC, Camargo SE, Mancini MN. Smear layer removal by auxiliary chemical substances in biomechanical preparation: a scanning electron microscope study. J Endod. 2008;34:1396–400.CrossRefPubMed
4.
go back to reference Ng YL, Mann V, Gulabivala K. A prospective study of the factors affecting outcomes of nonsurgical root canal treatment: part 1: periapical health. Int Endod J. 2011;44:583–609.CrossRefPubMed Ng YL, Mann V, Gulabivala K. A prospective study of the factors affecting outcomes of nonsurgical root canal treatment: part 1: periapical health. Int Endod J. 2011;44:583–609.CrossRefPubMed
5.
go back to reference Pintor AV, Dos Santos MR, Ferreira DM, Barcelos R, Primo LG, Maia LC. Does smear layer removal influence root canal therapy outcome? A systematic review. J Clin Pediatr Dent. 2016;40:1–7.CrossRefPubMed Pintor AV, Dos Santos MR, Ferreira DM, Barcelos R, Primo LG, Maia LC. Does smear layer removal influence root canal therapy outcome? A systematic review. J Clin Pediatr Dent. 2016;40:1–7.CrossRefPubMed
6.
go back to reference Neelakantan P, Devaraj S, Jagannathan N. Histologic assessment of debridement of the root canal isthmus of mandibular molars by irrigant activation techniques ex vivo. J Endod. 2016;42:1268–72.CrossRefPubMed Neelakantan P, Devaraj S, Jagannathan N. Histologic assessment of debridement of the root canal isthmus of mandibular molars by irrigant activation techniques ex vivo. J Endod. 2016;42:1268–72.CrossRefPubMed
7.
go back to reference De-Deus G, Reis C, Paciornik S. Critical appraisal of published smear layer-removal studies: methodological issues. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;112:531–43.CrossRefPubMed De-Deus G, Reis C, Paciornik S. Critical appraisal of published smear layer-removal studies: methodological issues. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;112:531–43.CrossRefPubMed
8.
go back to reference Lottanti S, Gautschi H, Sener B, Zehnder M. Effects of ethylenediaminetetraacetic, etidronic and peracetic acid irrigation on human root dentin and the smear layer. Int Endod J. 2009;42:335–43.CrossRefPubMed Lottanti S, Gautschi H, Sener B, Zehnder M. Effects of ethylenediaminetetraacetic, etidronic and peracetic acid irrigation on human root dentin and the smear layer. Int Endod J. 2009;42:335–43.CrossRefPubMed
9.
go back to reference Mancini M, Cerroni L, Iorio L, Armellin E, Conte G, Cianconi L. Smear layer removal and canal cleanliness using different irrigation systems (EndoActivator, EndoVac, and passive ultrasonic irrigation): field emission scanning electron microscopic evaluation in an in vitro study. J Endod. 2013;39:1456–60.CrossRefPubMed Mancini M, Cerroni L, Iorio L, Armellin E, Conte G, Cianconi L. Smear layer removal and canal cleanliness using different irrigation systems (EndoActivator, EndoVac, and passive ultrasonic irrigation): field emission scanning electron microscopic evaluation in an in vitro study. J Endod. 2013;39:1456–60.CrossRefPubMed
10.
go back to reference Blank-Goncalves LM, Nabeshima CK, Martins GH, Machado ME. Qualitative analysis of the removal of the smear layer in the apical third of curved roots: conventional irrigation versus activation systems. J Endod. 2011;37:1268–71.CrossRefPubMed Blank-Goncalves LM, Nabeshima CK, Martins GH, Machado ME. Qualitative analysis of the removal of the smear layer in the apical third of curved roots: conventional irrigation versus activation systems. J Endod. 2011;37:1268–71.CrossRefPubMed
11.
go back to reference Schmidt TF, Teixeira CS, Felippe MC, Felippe WT, Pashley DH, Bortoluzzi EA. Effect of ultrasonic activation of irrigants on smear layer removal. J Endod. 2015;41:1359–63.CrossRefPubMed Schmidt TF, Teixeira CS, Felippe MC, Felippe WT, Pashley DH, Bortoluzzi EA. Effect of ultrasonic activation of irrigants on smear layer removal. J Endod. 2015;41:1359–63.CrossRefPubMed
12.
go back to reference Tay FR, Gu LS, Schoeffel GJ, Wimmer C, Susin L, Zhang K, Arun SN, Kim J, Looney SW, Pashley DH. Effect of vapor lock on root canal debridement by using a side-vented needle for positive-pressure irrigant delivery. J Endod. 2010;36:745–50.CrossRefPubMedPubMedCentral Tay FR, Gu LS, Schoeffel GJ, Wimmer C, Susin L, Zhang K, Arun SN, Kim J, Looney SW, Pashley DH. Effect of vapor lock on root canal debridement by using a side-vented needle for positive-pressure irrigant delivery. J Endod. 2010;36:745–50.CrossRefPubMedPubMedCentral
13.
go back to reference Hulsmann M, Rummelin C, Schafers F. Root canal cleanliness after preparation with different endodontic handpieces and hand instruments: a comparative SEM investigation. J Endod. 1997;23:301–6.CrossRefPubMed Hulsmann M, Rummelin C, Schafers F. Root canal cleanliness after preparation with different endodontic handpieces and hand instruments: a comparative SEM investigation. J Endod. 1997;23:301–6.CrossRefPubMed
14.
go back to reference Vasiliadis L, Darling AI, Levers BG. The amount and distribution of sclerotic human root dentin. Arch Oral Biol. 1983;28:645–9.CrossRefPubMed Vasiliadis L, Darling AI, Levers BG. The amount and distribution of sclerotic human root dentin. Arch Oral Biol. 1983;28:645–9.CrossRefPubMed
15.
go back to reference Basmadjian-Charles CL, Farge P, Bourgeois DM, Lebrun T. Factors influencing the long-term results of endodontic treatment: a review of the literature. Int Dent J. 2002;52:81–6.CrossRefPubMed Basmadjian-Charles CL, Farge P, Bourgeois DM, Lebrun T. Factors influencing the long-term results of endodontic treatment: a review of the literature. Int Dent J. 2002;52:81–6.CrossRefPubMed
16.
go back to reference Gu LS, Kim JR, Ling J, Choi KK, Pashley DH, Tay FR. Review of contemporary irrigant agitation techniques and devices. J Endod. 2009;35:791–804.CrossRefPubMed Gu LS, Kim JR, Ling J, Choi KK, Pashley DH, Tay FR. Review of contemporary irrigant agitation techniques and devices. J Endod. 2009;35:791–804.CrossRefPubMed
17.
go back to reference George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod. 2005;31:867–72.CrossRefPubMed George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod. 2005;31:867–72.CrossRefPubMed
18.
go back to reference Baumgartner JC, Mader CL. A scanning electron microscopic evaluation of four root canal irrigation regimens. J Endod. 1987;13:147–57.CrossRefPubMed Baumgartner JC, Mader CL. A scanning electron microscopic evaluation of four root canal irrigation regimens. J Endod. 1987;13:147–57.CrossRefPubMed
19.
go back to reference Saunders WP, Saunders EM. The effect of smear layer upon the coronal leakage of gutta-percha fillings and a glass ionomer sealer. Int Endod J. 1992;25:245–9.CrossRefPubMed Saunders WP, Saunders EM. The effect of smear layer upon the coronal leakage of gutta-percha fillings and a glass ionomer sealer. Int Endod J. 1992;25:245–9.CrossRefPubMed
20.
go back to reference Yang SE, Bae KS. Scanning electron microscopy study of the adhesion of Prevotella nigrescens to the dentin of prepared root canals. J Endod. 2002;28:433–7.CrossRefPubMed Yang SE, Bae KS. Scanning electron microscopy study of the adhesion of Prevotella nigrescens to the dentin of prepared root canals. J Endod. 2002;28:433–7.CrossRefPubMed
21.
go back to reference Cameron JA. The use of ultrasound for the removal of the smear layer. The effect of sodium hypochlorite concentration; SEM study. Aust Dent J. 1988;33:193–200.CrossRefPubMed Cameron JA. The use of ultrasound for the removal of the smear layer. The effect of sodium hypochlorite concentration; SEM study. Aust Dent J. 1988;33:193–200.CrossRefPubMed
22.
go back to reference Rodig T, Dollmann S, Konietschke F, Drebenstedt S, Hulsmann M. Effectiveness of different irrigant agitation techniques on debris and smear layer removal in curved root canals: a scanning electron microscopy study. J Endod. 2010;36:1983–7.CrossRefPubMed Rodig T, Dollmann S, Konietschke F, Drebenstedt S, Hulsmann M. Effectiveness of different irrigant agitation techniques on debris and smear layer removal in curved root canals: a scanning electron microscopy study. J Endod. 2010;36:1983–7.CrossRefPubMed
23.
go back to reference Prado M, Gusman H, Gomes BP, Simao RA. Scanning electron microscopic investigation of the effectiveness of phosphoric acid in smear layer removal when compared with EDTA and citric acid. J Endod. 2011;37:255–8.CrossRefPubMed Prado M, Gusman H, Gomes BP, Simao RA. Scanning electron microscopic investigation of the effectiveness of phosphoric acid in smear layer removal when compared with EDTA and citric acid. J Endod. 2011;37:255–8.CrossRefPubMed
24.
go back to reference de Gregorio C, Estevez R, Cisneros R, Paranjpe A, Cohenca N. Efficacy of different irrigation and activation systems on the penetration of sodium hypochlorite into simulated lateral canals and up to working length: an in vitro study. J Endod. 2010;36:1216–21.CrossRefPubMed de Gregorio C, Estevez R, Cisneros R, Paranjpe A, Cohenca N. Efficacy of different irrigation and activation systems on the penetration of sodium hypochlorite into simulated lateral canals and up to working length: an in vitro study. J Endod. 2010;36:1216–21.CrossRefPubMed
25.
26.
go back to reference Mozo S, Llena C, Forner L. Review of ultrasonic irrigation in endodontics: increasing action of irrigating solutions. Med Oral Patol Oral Cir Bucal. 2012;17:e512-6.PubMed Mozo S, Llena C, Forner L. Review of ultrasonic irrigation in endodontics: increasing action of irrigating solutions. Med Oral Patol Oral Cir Bucal. 2012;17:e512-6.PubMed
27.
go back to reference Jiang LM, Verhaagen B, Versluis M, van der Sluis LW. Evaluation of a sonic device designed to activate irrigant in the root canal. J Endod. 2010;36:143–6.CrossRefPubMed Jiang LM, Verhaagen B, Versluis M, van der Sluis LW. Evaluation of a sonic device designed to activate irrigant in the root canal. J Endod. 2010;36:143–6.CrossRefPubMed
28.
go back to reference Moorer WR, Wesselink PR. Factors promoting the tissue dissolving capability of sodium hypochlorite. Int Endod J. 1982;15:187–96.CrossRefPubMed Moorer WR, Wesselink PR. Factors promoting the tissue dissolving capability of sodium hypochlorite. Int Endod J. 1982;15:187–96.CrossRefPubMed
29.
go back to reference Saber Sel D, Hashem AA. Efficacy of different final irrigation activation techniques on smear layer removal. J Endod. 2011;37:1272–5.CrossRef Saber Sel D, Hashem AA. Efficacy of different final irrigation activation techniques on smear layer removal. J Endod. 2011;37:1272–5.CrossRef
30.
go back to reference Wu L, Mu Y, Deng X, Zhang S, Zhou D. Comparison of the effect of four decalcifying agents combined with 60 °C 3% sodium hypochlorite on smear layer removal. J Endod. 2012;38:381–4.CrossRefPubMed Wu L, Mu Y, Deng X, Zhang S, Zhou D. Comparison of the effect of four decalcifying agents combined with 60 °C 3% sodium hypochlorite on smear layer removal. J Endod. 2012;38:381–4.CrossRefPubMed
31.
go back to reference Khademi A, Yazdizadeh M, Feizianfard M. Determination of the minimum instrumentation size for penetration of irrigants to the apical third of root canal systems. J Endod. 2006;32:417–20.CrossRefPubMed Khademi A, Yazdizadeh M, Feizianfard M. Determination of the minimum instrumentation size for penetration of irrigants to the apical third of root canal systems. J Endod. 2006;32:417–20.CrossRefPubMed
32.
go back to reference Lee SJ, Wu MK, Wesselink PR. The efficacy of ultrasonic irrigation to remove artificially placed dentin debris from different-sized simulated plastic root canals. Int Endod J. 2004;37:607–12.CrossRefPubMed Lee SJ, Wu MK, Wesselink PR. The efficacy of ultrasonic irrigation to remove artificially placed dentin debris from different-sized simulated plastic root canals. Int Endod J. 2004;37:607–12.CrossRefPubMed
33.
go back to reference Capar ID, Aydinbelge HA. Effectiveness of various irrigation activation protocols and the self-adjusting file system on smear layer and debris removal. Scanning. 2014;36:640–7.CrossRefPubMed Capar ID, Aydinbelge HA. Effectiveness of various irrigation activation protocols and the self-adjusting file system on smear layer and debris removal. Scanning. 2014;36:640–7.CrossRefPubMed
34.
go back to reference Malki M, Verhaagen B, Jiang LM, Nehme W, Naaman A, Versluis M, Wesselink P, van der Sluis L. Irrigant flow beyond the insertion depth of an ultrasonically oscillating file in straight and curved root canals: visualization and cleaning efficacy. J Endod. 2012;38:657–61.CrossRefPubMed Malki M, Verhaagen B, Jiang LM, Nehme W, Naaman A, Versluis M, Wesselink P, van der Sluis L. Irrigant flow beyond the insertion depth of an ultrasonically oscillating file in straight and curved root canals: visualization and cleaning efficacy. J Endod. 2012;38:657–61.CrossRefPubMed
35.
go back to reference Sabins RA, Johnson JD, Hellstein JW. A comparison of the cleaning efficacy of short-term sonic and ultrasonic passive irrigation after hand instrumentation in molar root canals. J Endod. 2003;29:674–8.CrossRefPubMed Sabins RA, Johnson JD, Hellstein JW. A comparison of the cleaning efficacy of short-term sonic and ultrasonic passive irrigation after hand instrumentation in molar root canals. J Endod. 2003;29:674–8.CrossRefPubMed
36.
go back to reference Klyn SL, Kirkpatrick TC, Rutledge RE. In vitro comparisons of debris removal of the EndoActivator system, the F file, ultrasonic irrigation, and NaOCl irrigation alone after hand-rotary instrumentation in human mandibular molars. J Endod. 2010;36:1367–71.CrossRefPubMed Klyn SL, Kirkpatrick TC, Rutledge RE. In vitro comparisons of debris removal of the EndoActivator system, the F file, ultrasonic irrigation, and NaOCl irrigation alone after hand-rotary instrumentation in human mandibular molars. J Endod. 2010;36:1367–71.CrossRefPubMed
37.
go back to reference Mader CL, Baumgartner JC, Peters DD. Scanning electron microscopic investigation of the smeared layer on root canal walls. J Endod. 1984;10:477–83.CrossRefPubMed Mader CL, Baumgartner JC, Peters DD. Scanning electron microscopic investigation of the smeared layer on root canal walls. J Endod. 1984;10:477–83.CrossRefPubMed
38.
go back to reference White RR, Goldman M, Lin PS. The influence of the smeared layer upon dentinal tubule penetration by endodontic filling materials. Part II. J Endod. 1987;13:369–74.CrossRefPubMed White RR, Goldman M, Lin PS. The influence of the smeared layer upon dentinal tubule penetration by endodontic filling materials. Part II. J Endod. 1987;13:369–74.CrossRefPubMed
Metadata
Title
Effectiveness of irrigation strategies on the removal of the smear layer from root canal dentin
Authors
Prasanna Neelakantan
Hani F. Ounsi
Sharmila Devaraj
Gary S. P. Cheung
Simone Grandini
Publication date
01-04-2019
Publisher
Springer Japan
Published in
Odontology / Issue 2/2019
Print ISSN: 1618-1247
Electronic ISSN: 1618-1255
DOI
https://doi.org/10.1007/s10266-018-0373-2

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