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Clinical Oral Investigations
Published in: Clinical Oral Investigations 8/2016

01-11-2016 | Original Article

Effect of therapeutic doses of radiotherapy on the organic and inorganic contents of the deciduous enamel: an in vitro study

Authors: Elza Maria de Sá Ferreira, Luis Eduardo Silva Soares, Héliton Spíndola Antunes, Sofia Takeda Uemura, Patrícia da Silva Barbosa, Hélio Augusto Salmon Jr, Giselle Rodrigues de Sant’Anna

Published in: Clinical Oral Investigations | Issue 8/2016

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Abstract

Objectives

This study evaluated the effects of radiotherapy on the composition of deciduous teeth enamel using micro-energy-dispersive X-ray fluorescence and Fourier transform Raman spectroscopy before and after a pH cycling process.

Materials and methods

Ten deciduous molars were sectioned and divided into two groups (n = 10). The radiotherapy group (RT) was irradiated with 54 Gy at 2 Gy/day, 5 days per week for 5 weeks and 2 days, and the normal group (N) was not irradiated. The RT group was evaluated before radiotherapy (RTb), after radiotherapy (RTa), and after radiotherapy and pH cycling (RTc). The normal group was evaluated before (N) and after pH cycling (Nc). The weight percentage (wt%) of calcium (Ca), phosphorus (P), and organic content; the Ca/P ratio; and the integrated area of the Raman bands relative to the organic, carbonate, and phosphate contents were also evaluated.

Results

The exclusive use of RT reduced the organic content of the enamel (p = 0.000). The RTc group exhibited a decrease in P wt% (p = 0.016), an increase in the Ca/P ratio (p = 0.000), and a reduction in the integrated area of the phosphate band (p = 0.046). An increase in the Ca/P ratio (p = 0.000) and a reduction in the areas of the carbonate and phosphate bands were found in the RTb/RTc treatments.

Conclusions

RT application at a therapeutic dose reduced the organic content of the deciduous enamel.

Clinical relevance

Preventive measures should be included in the patient treatment protocol because of RT-induced chemical changes to the deciduous enamel.
Appendix
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Metadata
Title
Effect of therapeutic doses of radiotherapy on the organic and inorganic contents of the deciduous enamel: an in vitro study
Authors
Elza Maria de Sá Ferreira
Luis Eduardo Silva Soares
Héliton Spíndola Antunes
Sofia Takeda Uemura
Patrícia da Silva Barbosa
Hélio Augusto Salmon Jr
Giselle Rodrigues de Sant’Anna
Publication date
01-11-2016
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 8/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-015-1686-y

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