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Clinical Oral Investigations

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01-11-2016 | Original Article

In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

Authors: Karolina Jurczyk, Sandor Nietzsche, Claudia Ender, Anton Sculean, Sigrun Eick

Published in: Clinical Oral Investigations | Issue 8/2016

Abstract

Objectives

The aim of the present study was to assess the antimicrobial activity of a sodium hypochlorite formulation including its components against bacteria associated with periodontal disease.

Materials and methods

Sodium hypochlorite formulation (NaOCl gel), its components sodium hypochlorite (NaOCl), and the activating vehicle were compared with 0.1 % chlorhexidine digluconate (CHX) solution. The antimicrobial activity was proven by determination of minimal inhibitory concentrations (MIC), minimal bactericidal concentrations, and killing assays. Furthermore, the influence on formation as well as on a 4-day-old 6-species biofilm was tested.

Results

Except for one strain (Parvimonas micra ATCC 33270 in case of NaOCl gel), the MICs both of the CHX solution and NaOCl gel did not exceed 10 % of the formulations’ concentration. In general, MICs of the NaOCl gel were equal as of the CHX solution against Gram-negatives but higher against Gram-positive bacteria. CHX but not NaOCl gel clearly inhibited biofilm formation; however, the activity of NaOCl gel was more remarkable on a 4-day-old biofilm. NaOCl killed bacteria in the biofilm and interfered with the matrix.

Conclusions

The NaOCl gel acts antimicrobial in particular against Gram-negative species associated with periodontitis. Moreover, its component NaOCl hypochlorite is able to alter biofilm matrices.

Clinical relevance

The NaOCl gel may represent a potential alternative for adjunctive topical antimicrobial treatment in periodontitis.

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Title: In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis
Authors: Karolina Jurczyk
Sandor Nietzsche
Claudia Ender
Anton Sculean
Sigrun Eick
Publication date: 01-11-2016
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 8/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-1711-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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