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Calcified Tissue International
Published in: Calcified Tissue International 3/2019

01-03-2019 | Original Research

Effect of Long-Term Green Tea Polyphenol Supplementation on Bone Architecture, Turnover, and Mechanical Properties in Middle-Aged Ovariectomized Rats

Authors: Chwan-Li Shen, Brenda J. Smith, Jiliang Li, Jay J. Cao, Xiao Song, Maria F. Newhardt, Kylie A. Corry, Michael D. Tomison, Lili Tang, Jia-Sheng Wang, Ming-Chien Chyu

Published in: Calcified Tissue International | Issue 3/2019

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Abstract

We investigated the effects of 6-month green tea polyphenols (GTP) supplementation on bone architecture, turnover, and mechanical properties in middle-aged ovariectomized (OVX) rats. Female rats were sham-operated (n = 39, 13/group) or OVX (n = 143, 13/group). Sham-control and OVX-control rats (n = 39) receiving no GTP were assigned for sample collection at baseline, 3, or 6 months. The remaining OVX rats (n = 104) were randomized to 0.15%, 0.5%, 1%, and 1.5% (g/dL) GTP for 3 or 6 months. Blood and bone samples were collected. Relative to the OVX-control group, GTP (1% and 1.5%) lowered serum procollagen type 1 N-terminal propeptide at 3 and 6 months, C-terminal telopeptides of type I collagen at 3 months, and insulin-like growth factor-I at 6 months. GTP did not affect bone mineral content and density. At 6 months, no dose of GTP positively affected trabecular bone volume based on microCT, but a higher cortical thickness and improved biomechanical properties of the femur mid-diaphysis was observed in the 1.5% GTP-treated group. At 3 and 6 months, GTP (0.5%, 1%, and 1.5%) had lower rates of trabecular bone formation and resorption than the OVX-control group, but the inhibitory effects of GTP on periosteal and endocortical bone mineralization and formation at the tibial midshaft were only evident at 3 months. GTP at higher doses suppressed bone turnover in the trabecular and cortical bone of OVX rats and resulted in improved cortical bone structural and biomechanical properties, although it was not effective in preventing the ovariectomy-induced dramatic cancellous bone loss.
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Metadata
Title
Effect of Long-Term Green Tea Polyphenol Supplementation on Bone Architecture, Turnover, and Mechanical Properties in Middle-Aged Ovariectomized Rats
Authors
Chwan-Li Shen
Brenda J. Smith
Jiliang Li
Jay J. Cao
Xiao Song
Maria F. Newhardt
Kylie A. Corry
Michael D. Tomison
Lili Tang
Jia-Sheng Wang
Ming-Chien Chyu
Publication date
01-03-2019
Publisher
Springer US
Published in
Calcified Tissue International / Issue 3/2019
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI
https://doi.org/10.1007/s00223-018-0489-y

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