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01-06-2019 | Original Article

Effect of light-emitting diodes, platelet-rich plasma, and their combination on the activity of sheep tenocytes

Authors: Jihad A. M. Alzyoud, Saleh A. Al Najjar, Seham Talat, Basha’er Abu-Irmaileh, Yasser Bustanji, Abd Al-Rahman S. Al-Shudiefat

Published in: Lasers in Medical Science | Issue 4/2019

Abstract

Healthy tendons play an important role in joint movements and subjected to a group of pathologies called tendinopathy due to multiple factors. Tendons have a slowly repairing process due to the low vascularity and cellularity. Treatment options aimed at potentiating the healing response and relieving symptoms. Phototherapy and platelet-rich plasma were novel treatment modalities in tendons based on photobiomodulation and growth factors during healing, and the results were encouraging suggesting calibrating treatment parameters. This study utilizes cell culture to explore the potential effect of light-emitting diode and/or growth factors in the form of platelet-rich plasma (PRP) on the activity of tenocytes isolated from sheep Achilles tendons by measuring the cell metabolism and cell mobility using cell viability and migration assays to proof safety and confirm activity. Results showed that sheep tenocyte-cultured groups treated with 5% platelet-rich plasma alone or combined with 4 J/cm² light-emitting diode have increased viability significantly when compared to control group after a 48 h, while light-emitting diode treatment has not decreased cell migration significantly when compared with control. Result suggests that using platelet-rich plasma alone or combined with light-emitting diode might have potential to enhance healing response at the conditions applied. PRP could enhance proliferation while LED could enhance migration and proliferation. Further research is needed at longer durations.

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Title: Effect of light-emitting diodes, platelet-rich plasma, and their combination on the activity of sheep tenocytes
Authors: Jihad A. M. Alzyoud
Saleh A. Al Najjar
Seham Talat
Basha’er Abu-Irmaileh
Yasser Bustanji
Abd Al-Rahman S. Al-Shudiefat
Publication date: 01-06-2019
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 4/2019
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-018-2657-8

At a glance: The STEP trials

Springer Medicine

Effect of light-emitting diodes, platelet-rich plasma, and their combination on the activity of sheep tenocytes

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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