Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
BMC Ophthalmology
Published in: BMC Ophthalmology 1/2016

Open Access 01-12-2016 | Research article

Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion

Authors: Wenting Cai, Qingquan Wei, Qingyu Liu, Chengda Ren, Junling Liu, Ruiling Zhang, Mengmei He, Qianyi Wang, Yaru Du, Jing Yu

Published in: BMC Ophthalmology | Issue 1/2016

Login to get access

Abstract

Background

To evaluate the effect of bradykinin (BK) on TGF-β1-induced retinal pigment epithelial (RPE) cell proliferation and extracellular matrix secretion and to elucidate the relationship between BK and the Erk/Akt signaling pathway.

Methods

The effects of BK on TGF-β1-induced RPE cell proliferation were examined via CCK-8 assay. Cell culture supernatant collagen I concentrations were measured via ELISA. Fibronectin (Fn), matrix metalloproteinase-2 (MMP-2) and MMP-9 mRNA and protein expression levels were measured via q-PCR and Western blotting, respectively. Changes in Akt/Erk phosphorylation induced by BK and HOE-140 were evaluated via Western blotting.

Results

TGF-β1 stimulated ARPE-19 cell proliferation, which was inhibited by BK, whose effects were inhibited by HOE-140. BK inhibited TGF-β1-induced collagen I, Fn and MMP-2 secretion in RPE cells, and these effects were inhibited by HOE-140. BK also inhibited TGF-β1-induced Akt phosphorylation in RPE cells, and these effects were blocked by HOE-140. BK had no significant effect on Erk-mediated signaling.

Conclusions

The findings from this study indicate that BK could be novel therapeutic targets for the treatment of PVR.
Literature
1.
2.
3.
Ciprian D. The pathogeny of proliferative vitreoretinopathy. Rom J Ophthalmol. 2015;59(2):88–92.PubMed
4.
5.
6.
7.
8.
Kimoto K, Nakatsuka K, Matsuo N, Yoshioka H. p38 MAPK mediates the expression of type I collagen induced by TGF-beta 2 in human retinal pigment epithelial cells ARPE-19. Invest Ophthalmol Vis Sci. 2004;45:2431–7.CrossRefPubMed
9.
10.
11.
12.
13.
14.
Kon CH, Occleston NL, Charteris D, Daniels J, Aylward GW, Khaw PT. A prospective study of matrix metalloproteinases in proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci. 1998;39(8):1524–9.PubMed
15.
16.
17.
Zeng J, Jiang DY. The relationship between matrix metalloproteinases and inhibitors with vitreoretinal diseases. Int J Ophthalmol Vol. 2000;2(2):83–6.
18.
19.
Nassar K, Grisanti S, Tura A, Lüke J, Lüke M, Soliman M, et al. A TGF-beta receptor 1 inhibitor for prevention of proliferative vitreoretinopathy. Exp Eye Res. 2014;123:72–86.CrossRefPubMed
20.
Pasquale LR, Dorman-Pease ME, Lutty GA, Quigley HA, Jampel HD. Immunolocalization of TGF-beta 1, TGF-beta 2, and TGF-beta 3 in the anterior segment of the human eye. Invest Ophthalmol Vis Sci. 1993;34(1):23–30.PubMed
21.
Zhao HM, Yu J, Sheng MJ. Experimental study of kallikrein-kinin system participating in proliferative vitreoretinopathy procedure. Chinese Journal of Experimental Ophthalmology. 2011;29:591–5.
22.
23.
24.
25.
Yu J, Liu F, Cui SJ, Liu Y, Song ZY, Cao H, et al. Vitreous proteomic analysis of proliferative vitreoretinopathy. Proteomics. 2008;8:3667–78.CrossRefPubMed
26.
Yu J, Wang F. Verify kininogen1 as molecular marker in the serum of proliferative vitreous retinopathy. Rec Adv Ophthal. 2009;29:561–4.
27.
28.
29.
Penn JW, Grobbelaar AO, Rolfe KJ. The role of the TGF-beta family in wound healing, burns and scarring: a review. Int J Burns Trauma. 2012;2:18–28.PubMedPubMedCentral
30.
31.
32.
33.
Qing QH, Yao YM. Research progress of TGF-βsignal pathway. J Med Sci. 2012;41(10):5–9.
34.
35.
Metadata
Title
Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion
Authors
Wenting Cai
Qingquan Wei
Qingyu Liu
Chengda Ren
Junling Liu
Ruiling Zhang
Mengmei He
Qianyi Wang
Yaru Du
Jing Yu
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Ophthalmology / Issue 1/2016
Electronic ISSN: 1471-2415
DOI
https://doi.org/10.1186/s12886-016-0373-3

Other articles of this Issue 1/2016

BMC Ophthalmology 1/2016 Go to the issue

Research article

The relationship between self-reported habitual exercise and visual field defect progression: a retrospective cohort study

Research article

Changes in intraocular pressure values measured with noncontact tonometer (NCT), ocular response analyzer (ORA) and corvis scheimpflug technology tonometer (CST) in the early phase after small incision lenticule extraction (SMILE)

Research article

Protective effect of etanercept, an inhibitor of tumor necrosis factor-α, in a rat model of retinal ischemia

Research article

Refractive shift of silicone oil tamponade in pseudophakic eye

Research article

The serum angiotensin converting enzyme and lysozyme levels in patients with ocular involvement of autoimmune and infectious diseases

Case report

Closantel; a veterinary drug with potential severe morbidity in humans