Skip to main content
Top
Published in: Clinical Oral Investigations 1/2006

01-03-2006

Effect of an oily calcium hydroxide suspension (Osteoinductal) on healing of intrabony periodontal defects. A pilot study in dogs

Authors: Frank Schwarz, Stefan-Ioan Stratul, Monika Herten, Brigitte Beck, Jürgen Becker, Anton Sculean

Published in: Clinical Oral Investigations | Issue 1/2006

Login to get access

Abstract

The aim of the present study was to evaluate histologically in dogs the effect of treating intrabony defects with an oily calcium hydroxide suspension (OCHS). Intrabony defects were surgically created bilaterally at the distal aspects of the maxillary first premolars and at the mesial aspects of the third premolars in two mongrel dogs. Subsequently, the defects were randomly treated with (a) access flap surgery followed by the application of an OCHS or (b) access flap surgery alone. After 8 weeks of healing, the animals were killed. Dissected blocks containing the experimental specimens were fixed in formalin, decalcified in EDTA, and embedded in paraffin. The formation of new cementum and bone was assessed histomorphometrically. In the control group, healing was predominantly characterized by the formation of a long junctional epithelium along the root surface and limited periodontal regeneration at the most apical part of the defect. The OCHS-treated defects consistently revealed periodontal regeneration (i.e., new periodontal ligament, new cementum with inserting collagen fibers, and new bone). Within the limits of the present study, it can be concluded that OCHS may favor periodontal regeneration in acute-type intrabony periodontal defects.
Literature
1.
go back to reference Brunsvold MA, Mellonig JT (1993) Bone grafts and periodontal regeneration. Periodontol 2000 1:80–91CrossRef Brunsvold MA, Mellonig JT (1993) Bone grafts and periodontal regeneration. Periodontol 2000 1:80–91CrossRef
2.
go back to reference Caton JG, Greenstein G (1993) Factors related to periodontal regeneration. Periodontol 2000 1:9–15CrossRef Caton JG, Greenstein G (1993) Factors related to periodontal regeneration. Periodontol 2000 1:9–15CrossRef
3.
go back to reference Caton J, Nyman S (1980) Histometric evaluation of periodontal surgery. I. The modified Widman flap procedure. J Clin Periodontol 7:212–223PubMedCrossRef Caton J, Nyman S (1980) Histometric evaluation of periodontal surgery. I. The modified Widman flap procedure. J Clin Periodontol 7:212–223PubMedCrossRef
4.
go back to reference Caton J, Nyman S, Zander H (1980) Histometric evaluation of periodontal surgery. II. Connective tissue attachment levels after four regenerative procedures. J Clin Periodontol 7:224–231PubMedCrossRef Caton J, Nyman S, Zander H (1980) Histometric evaluation of periodontal surgery. II. Connective tissue attachment levels after four regenerative procedures. J Clin Periodontol 7:224–231PubMedCrossRef
5.
go back to reference Caton J, Mota L, Gandini L, Laskaris B (1994) Non-human primate models for testing the efficacy and safety of periodontal regeneration procedures. J Periodontol 65:1143–1150PubMed Caton J, Mota L, Gandini L, Laskaris B (1994) Non-human primate models for testing the efficacy and safety of periodontal regeneration procedures. J Periodontol 65:1143–1150PubMed
6.
go back to reference Charles P, Nathalie S, Carine D, Alexandru G (2004) Calcium hydroxide and treatment of inflammatory inter-radicular bone resorption of non-vital deciduous molars. Rev Belge Med Dent 59:163–169PubMed Charles P, Nathalie S, Carine D, Alexandru G (2004) Calcium hydroxide and treatment of inflammatory inter-radicular bone resorption of non-vital deciduous molars. Rev Belge Med Dent 59:163–169PubMed
7.
go back to reference Claffey N, Polyzois I, Ziaka P (2004) An overview of nonsurgical and surgical therapy. Periodontol 2000 36:35–44PubMedCrossRef Claffey N, Polyzois I, Ziaka P (2004) An overview of nonsurgical and surgical therapy. Periodontol 2000 36:35–44PubMedCrossRef
8.
go back to reference Cochran DL, King GN, Schoolfield J, Velasquez-Plata D, Mellonig JT, Jones A (2003) The effect of enamel matrix proteins on periodontal regeneration as determined by histological analyses. J Periodontol 74:1043–1055PubMedCrossRef Cochran DL, King GN, Schoolfield J, Velasquez-Plata D, Mellonig JT, Jones A (2003) The effect of enamel matrix proteins on periodontal regeneration as determined by histological analyses. J Periodontol 74:1043–1055PubMedCrossRef
9.
go back to reference De Moor RJ, De Witte AM (2002) Periapical lesions accidentally filled with calcium hydroxide. Int Endod J 35:946–958PubMedCrossRef De Moor RJ, De Witte AM (2002) Periapical lesions accidentally filled with calcium hydroxide. Int Endod J 35:946–958PubMedCrossRef
10.
go back to reference De Rossi A, Silva LA, Leonardo MR, Rocha LB, Rossi MA (2005) Effect of rotary or manual instrumentation, with or without a calcium hydroxide/1% chlorhexidine intracanal dressing, on the healing of experimentally induced chronic periapical lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99:628–636PubMedCrossRef De Rossi A, Silva LA, Leonardo MR, Rocha LB, Rossi MA (2005) Effect of rotary or manual instrumentation, with or without a calcium hydroxide/1% chlorhexidine intracanal dressing, on the healing of experimentally induced chronic periapical lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99:628–636PubMedCrossRef
11.
go back to reference Evanov C, Liewehr F, Buxton TB, Joyce AP (2004) Antibacterial efficacy of calcium hydroxide and chlorhexidine gluconate irrigants at 37 degrees C and 46 degrees C. J Endod 30:653–657PubMedCrossRef Evanov C, Liewehr F, Buxton TB, Joyce AP (2004) Antibacterial efficacy of calcium hydroxide and chlorhexidine gluconate irrigants at 37 degrees C and 46 degrees C. J Endod 30:653–657PubMedCrossRef
12.
go back to reference Gottlow J, Nyman S, Karring T, Lindhe J (1984) New attachment formation as the result of controlled tissue regeneration. J Clin Periodontol 11:494–503PubMedCrossRef Gottlow J, Nyman S, Karring T, Lindhe J (1984) New attachment formation as the result of controlled tissue regeneration. J Clin Periodontol 11:494–503PubMedCrossRef
13.
go back to reference Haenni S, Schmidlin PR, Mueller B, Sener B, Zehnder M (2003) Chemical and antimicrobial properties of calcium hydroxide mixed with irrigating solutions. Int Endod J 36:100–105PubMedCrossRef Haenni S, Schmidlin PR, Mueller B, Sener B, Zehnder M (2003) Chemical and antimicrobial properties of calcium hydroxide mixed with irrigating solutions. Int Endod J 36:100–105PubMedCrossRef
14.
go back to reference Holland R, de Mello W, Nery MJ, Bernabe PF, de Souza V (1977) Reaction of human periapical tissue to pulp extirpation and immediate root canal filling with calcium hydroxide. J Endod 3:63–67PubMedCrossRef Holland R, de Mello W, Nery MJ, Bernabe PF, de Souza V (1977) Reaction of human periapical tissue to pulp extirpation and immediate root canal filling with calcium hydroxide. J Endod 3:63–67PubMedCrossRef
15.
go back to reference Isidor F, Karring T, Nyman S, Lindhe J (1985) New attachment–reattachment following reconstructive periodontal surgery. J Clin Periodontol 12:728–735PubMedCrossRef Isidor F, Karring T, Nyman S, Lindhe J (1985) New attachment–reattachment following reconstructive periodontal surgery. J Clin Periodontol 12:728–735PubMedCrossRef
16.
go back to reference Karring T, Nyman S, Gottlow J, Laurell L (1993) Development of the biological concept of guided tissue regeneration–animal and human studies. Periodontol 2000 1:26–35CrossRef Karring T, Nyman S, Gottlow J, Laurell L (1993) Development of the biological concept of guided tissue regeneration–animal and human studies. Periodontol 2000 1:26–35CrossRef
17.
go back to reference Karring T, Lindhe J, Cortellini P (1997) Regenerative periodontal therapy. Clinical periodontology and implant dentistry, 3rd edn. Munksgaard, Copenhagen, pp 597–646 Karring T, Lindhe J, Cortellini P (1997) Regenerative periodontal therapy. Clinical periodontology and implant dentistry, 3rd edn. Munksgaard, Copenhagen, pp 597–646
18.
go back to reference Klaiber B, Mittermayer C, Kaden P, Schwechten I (1981) Determination of the toxicity of root canal filling materials and their separate components in cell cultures. Dtsch Zahnarztl Z 36:212–216PubMed Klaiber B, Mittermayer C, Kaden P, Schwechten I (1981) Determination of the toxicity of root canal filling materials and their separate components in cell cultures. Dtsch Zahnarztl Z 36:212–216PubMed
19.
go back to reference Liang Y, Wang J, Yu S (2000) Mechanisms of bone repairment in periapical diseases: studies on adjusting bone metabolism with calcium hydroxide in vitro. Zhonghua Kou Qiang Yi Xue Za Zhi 35:112–114PubMed Liang Y, Wang J, Yu S (2000) Mechanisms of bone repairment in periapical diseases: studies on adjusting bone metabolism with calcium hydroxide in vitro. Zhonghua Kou Qiang Yi Xue Za Zhi 35:112–114PubMed
20.
go back to reference Lowenguth RA, Blieden TM (1993) Periodontal regeneration: root surface demineralization. Periodontol 2000 1:54–68CrossRef Lowenguth RA, Blieden TM (1993) Periodontal regeneration: root surface demineralization. Periodontol 2000 1:54–68CrossRef
21.
go back to reference Lynch SE, Williams RC, Polson AM, Howell TH, Reddy MS, Zappa UE, Antoniades HN (1989) A combination of platelet-derived and insulin-like growth factors enhances periodontal regeneration. J Clin Periodontol 16:545–548PubMedCrossRef Lynch SE, Williams RC, Polson AM, Howell TH, Reddy MS, Zappa UE, Antoniades HN (1989) A combination of platelet-derived and insulin-like growth factors enhances periodontal regeneration. J Clin Periodontol 16:545–548PubMedCrossRef
22.
go back to reference Nyman S, Lindhe J, Karring T, Rylander H (1982) New attachment following surgical treatment of human periodontal disease. J Clin Periodontol 9:290–296PubMedCrossRef Nyman S, Lindhe J, Karring T, Rylander H (1982) New attachment following surgical treatment of human periodontal disease. J Clin Periodontol 9:290–296PubMedCrossRef
23.
go back to reference Sculean A, Donos N, Brecx M, Reich E, Karring T (2000) Treatment of intrabony defects with guided tissue regeneration and enamel-matrix-proteins. An experimental study in monkeys. J Clin Periodontol 27:466–472PubMedCrossRef Sculean A, Donos N, Brecx M, Reich E, Karring T (2000) Treatment of intrabony defects with guided tissue regeneration and enamel-matrix-proteins. An experimental study in monkeys. J Clin Periodontol 27:466–472PubMedCrossRef
24.
go back to reference Sculean A, Stavropoulos A, Berakdar M, Windisch P, Karring T, Brecx M (2005) Formation of human cementum following different modalities of regenerative therapy. Clin Oral Investig 9:58–64PubMedCrossRef Sculean A, Stavropoulos A, Berakdar M, Windisch P, Karring T, Brecx M (2005) Formation of human cementum following different modalities of regenerative therapy. Clin Oral Investig 9:58–64PubMedCrossRef
25.
go back to reference Tepel J, Darwisch el Sawaf M, Hoppe W (1994) Reaction of inflamed periapical tissue to intracanal medicaments and root canal sealers. Endod Dent Traumatol 10:233–238PubMedCrossRef Tepel J, Darwisch el Sawaf M, Hoppe W (1994) Reaction of inflamed periapical tissue to intracanal medicaments and root canal sealers. Endod Dent Traumatol 10:233–238PubMedCrossRef
26.
go back to reference Tronstad L (1988) Root resorption-etiology, terminology and clinical manifestations. Endod Dent Traumatol 4:241–252PubMedCrossRef Tronstad L (1988) Root resorption-etiology, terminology and clinical manifestations. Endod Dent Traumatol 4:241–252PubMedCrossRef
27.
go back to reference Wikesjö UM, Selvig KA, Zimmerman G, Nilveus R (1991) Periodontal repair in dogs: healing in experimentally created chronic periodontal defects. J Periodontol 62:258–263PubMed Wikesjö UM, Selvig KA, Zimmerman G, Nilveus R (1991) Periodontal repair in dogs: healing in experimentally created chronic periodontal defects. J Periodontol 62:258–263PubMed
Metadata
Title
Effect of an oily calcium hydroxide suspension (Osteoinductal) on healing of intrabony periodontal defects. A pilot study in dogs
Authors
Frank Schwarz
Stefan-Ioan Stratul
Monika Herten
Brigitte Beck
Jürgen Becker
Anton Sculean
Publication date
01-03-2006
Publisher
Springer-Verlag
Published in
Clinical Oral Investigations / Issue 1/2006
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-005-0019-y

Other articles of this Issue 1/2006

Clinical Oral Investigations 1/2006 Go to the issue

Calendar of Events

Calendar of Events