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Inflammation Research
Published in: Inflammation Research 12/2020

01-12-2020 | Edema | Original Research Paper

Methyl gallate attenuates inflammation induced by Toll-like receptor ligands by inhibiting MAPK and NF-Κb signaling pathways

Authors: Luana Barbosa Correa, Leonardo Noboru Seito, Marília F. Manchope, Waldiceu A. Verri Jr, Thiago Mattar Cunha, Maria G. Henriques, Elaine Cruz Rosas

Published in: Inflammation Research | Issue 12/2020

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Abstract

Objective and design

Methyl gallate (MG) is a prevalent polyphenol in the plant kingdom, which may be related to the effects of several medicinal plants. Although it is widely reported that polyphenols have therapeutic effects, there are few studies demonstrating that MG has anti-inflammatory action. This study aimed to investigate the molecular mechanism behind the anti-inflammatory activity of MG and its effect on hyperalgesia.

Methods

Swiss mice were pretreated orally with different doses of MG and subjected to i.pl. injection of zymosan to induce paw edema. RAW264.7 macrophages and BMDMs stimulated with different TLR agonists such as zymosan, LPS, or Pam3CSK4 were used to investigate the molecular mechanisms of MG

Results

MG inhibits zymosan-induced paw edema and hyperalgesia and modulates molecular pathways crucial for inflammation development. Pretreatment with MG inhibited cytokines production and NF-κB activity by RAW 264.7 cells stimulated with zymosan, Pam3CSK4 or LPS, but not with PMA. Moreover, pretreatment with MG decreased IκB degradation, nuclear translocation of NF-κBp65, c-jun and c-fos and ERK1/2, p38 and JNK phosphorylation.

Conclusion

Thus, the results of this study demonstrate that MG has a promising anti-inflammatory effect and suggests an explanation of its mechanism of action through the inhibition of NF-κB signaling and the MAPK pathway.
Appendix
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Metadata
Title
Methyl gallate attenuates inflammation induced by Toll-like receptor ligands by inhibiting MAPK and NF-Κb signaling pathways
Authors
Luana Barbosa Correa
Leonardo Noboru Seito
Marília F. Manchope
Waldiceu A. Verri Jr
Thiago Mattar Cunha
Maria G. Henriques
Elaine Cruz Rosas
Publication date
01-12-2020
Publisher
Springer International Publishing
Keyword
Edema
Published in
Inflammation Research / Issue 12/2020
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-020-01407-0

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