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01-06-2013 | Research Paper

Ecto-5′-nucleotidase (CD73) promotes tumor angiogenesis

Authors: Li Wang, Shaoxian Tang, Yingjian Wang, Siguang Xu, Jerry Yu, Xiuling Zhi, Zhouluo Ou, Jiayin Yang, Ping Zhou, Zhimin Shao

Published in: Clinical & Experimental Metastasis | Issue 5/2013

Abstract

Angiogenesis is essential for tumor growth, progression and metastasis. Studies indicate that expression and activity of ecto-5′-nucleotidase (CD73) are elevated in metastatic carcinomas. Our previous studies found that angiogenesis of tumor xenografts was decreased when the activity of CD73 in cancer cells was inhibited, implying that this enzyme is involved in tumor angiogenesis. To elucidate the mechanism, we investigated CD73 influence on tumor angiogenesis in both in vitro assays and in tumor bearing mice. We found that capillary-like structures were formed more in CD73^+/+ pulmonary microvascular endothelial cells (PMECs) than CD73^−/− PMECs, and this was more pronounced when the cells were cultured in cancer-conditioned medium. Meanwhile, CD73 decreased endothelial cells adhesion to collagen IV and promoted migration. Additionally, the extent of tumor angiogenesis and the size of tumors were greater in CD73^+/+ mice than in CD73^−/− mice. Thus, we concluded that CD73 can promote endothelial cells forming new vessels in cancer condition, facilitating tumor growth and hematogenous metastasis.

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Title: Ecto-5′-nucleotidase (CD73) promotes tumor angiogenesis
Authors: Li Wang
Shaoxian Tang
Yingjian Wang
Siguang Xu
Jerry Yu
Xiuling Zhi
Zhouluo Ou
Jiayin Yang
Ping Zhou
Zhimin Shao
Publication date: 01-06-2013
Publisher: Springer Netherlands
Published in: Clinical & Experimental Metastasis / Issue 5/2013
Print ISSN: 0262-0898
Electronic ISSN: 1573-7276
DOI: https://doi.org/10.1007/s10585-013-9571-z

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