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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Research

Early tissue and healing responses after maxillary sinus augmentation using horizontal platelet rich fibrin bone blocks

Authors: Shimin Yu, Yinping Tian BD, Yan Wei BD, Mengge Feng BD, Sensen Li BMed, Guoyong Tong BMed, Zhouqing Yu BMed, Richard J. Miron, Yufeng Zhang, Zaibo Yang, Yulan Wang

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Background

The effects of horizontal platelet-rich fibrin (H-PRF) bone block on the healing and immune response during sinus augmentation have not been fully investigated histologically at early time points.

Methods

Eighteenth male New Zealand white rabbits underwent bilateral sinus augmentation and were divided into two groups: deproteinized bovine bone mineral (DBBM) alone and H-PRF + DBBM (H-PRF bone block) group. Maxilla samples were collected at 3, 7 and 14 days post sinus augmentation procedures and analyzed using histological staining for the number of inflammatory cells, new blood vessels and evidence for early osteoclast bone turnover/remodeling. Furthermore, the effects of H-PRF bone blocks on the migration of osteoblasts and THP-1 macrophages were evaluated using a Transwell assay in vitro.

Results

A higher number of immune cells were found in the H-PRF bone block group at 3 and 7 days post-surgery when compared to the DBBM alone group,most notably in the regions close to the mucosal lining and bone plates. Furthermore, a significantly greater number of new blood vessel formations and early signs of osteoclast development were found in the H-PRF bone block group at 14 days. The in vitro transwell assay further confirmed that culture medium from H-PRF bone block markedly promote the migration of osteoblasts and THP-1 macrophages.

Conclusions

The findings from this study have shown that H-PRF bone block is capable of increasing early immune cell infiltration leading to the acceleration of neovascularization and speeding the process of bone metabolism in vivo following maxillary sinus grafting with DBBM.
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Metadata
Title
Early tissue and healing responses after maxillary sinus augmentation using horizontal platelet rich fibrin bone blocks
Authors
Shimin Yu
Yinping Tian BD
Yan Wei BD
Mengge Feng BD
Sensen Li BMed
Guoyong Tong BMed
Zhouqing Yu BMed
Richard J. Miron
Yufeng Zhang
Zaibo Yang
Yulan Wang
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03228-z

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