Published in:
01-07-2005 | Original article
Early peritoneal macrophage function after laparoscopic surgery compared with laparotomy in a mouse mode
Published in: Surgical Endoscopy | Issue 7/2005
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Background
The authors previously demonstrated postoperative preservation of the immune function measured by delayed-type skin reaction and tumor growth after laparoscopic surgery, as compared with laparotomy. For further elucidation of the origin of the demonstrated immune preservation, peritoneal macrophage (PMo) function was investigated 1 h after different surgical procedures.
Methods
Female NMRI mice were divided into five groups: anesthesia only, abdominal skin incision, laparotomy, peritoneal carbon dioxide (CO2) insufflation, and peritoneal air insufflation. Escherichia Coli phagocytosis, tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1), and interleukin-10 (IL-10) release of isolated PMo were investigated.
Results
All invasive interventions reduced the PMo phagocytosis by factors of approximately 2 to 4.7, as compared with the sham control group. Spontaneous ex vivo TNF-α release was significantly increased whenever the abdominal cavity was exposed to ambient air. The macrophage’s ability to release TNF-α after E. coli exposure was diminished in the abdominal air exposure groups, as compared with the CO2 insufflation group.
Conclusion
Reduced phagocytosis 1 h after surgical interventions suggests a contribution of PMo to the altered immune function. When exposed to CO2, PMo show a decreased basal TNF-α release. However, PMo also show an increased TNF-α release after a second immune stimulation (E. coli), suggesting a greater competency of interaction in an immune defense reaction after CO2 exposure.