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European Radiology

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01-09-2014 | Ultrasound

Dynamic contrast–enhanced ultrasonographic (DCE-US) assessment of the early response after combined gemcitabine and HIFU with low-power treatment for the mouse xenograft model of human pancreatic cancer

Authors: Jung Hoon Kim, Haeri Kim, Young Jae Kim, Jae Young Lee, Joon Koo Han, Byung-Ihn Choi

Published in: European Radiology | Issue 9/2014

Abstract

Purpose

To assess therapeutic efficacy of gemcitabine and HIFU for a mouse model of pancreatic cancer, and the role of DCE-US for predicting early treatment response compared with pathology.

Materials and methods

In 48 PANC-1- nude mice (G1, HIFU_higher power [n = 14]; G2, gemcitabine [n = 12]; G3, combined gemcitabine and HIFU_low power [n = 12]; and G4, control [n = 10]), pulsed HIFU or gemcitabine therapy was used. DCE-US was performed 1 day before and after first treatment. Seven DCE-US perfusion parameters were obtained. Therapeutic efficacy was estimated using necrotic fraction and apoptosis. Correlation between tumour size and US perfusion parameters was analysed.

Results

Pathology results showed that combined gemcitabine and HIFU using low-power treatment had a more effective response than other treatments, including in the control group, i.e. necrotic fraction: 40.5 ± 4.9 vs. 16.9 ± 8.0, p = 0.000 and apoptosis: 44.3 ± 29.4 vs. 7.9 ± 4.9, p = 0.002. In this group, US perfusion parameters, including peak intensity (22.6 ± 22.6 vs. 9.6 ± 6.3, p = 0.002), AUC (961.8 ± 96.9 vs. 884.4 ± 91.4, p = 0.000), and AUC_out (799.9 ± 75.6 vs. 747.1 ± 77.9, p = 0.000), had significantly decreased 1 day following first treatment (p < 0.05). In addition, peak intensity, AUC, and AUC_out showed a tendency to decrease in treated groups. Alternatively, peak intensity, AUC, and AUC_out showed a tendency to increase in control group.

Conclusion

Gemcitabine and HIFU were more effective and safer than other treatments. US perfusion parameters were useful for predicting early therapeutic response 1 day following treatment.

Key points

• Recently, treatment of pancreatic cancer has changed based on a multidisciplinary approach.

• Combined gemcitabine_HIFU demonstrated more effective therapeutic response than other treatments.

• DCE-US is useful for predicting early therapeutic response 1 day after treatment.

• In the combined group, PI, AUC, and AUC ^out decreased 1 day after treatment.

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Title: Dynamic contrast–enhanced ultrasonographic (DCE-US) assessment of the early response after combined gemcitabine and HIFU with low-power treatment for the mouse xenograft model of human pancreatic cancer
Authors: Jung Hoon Kim
Haeri Kim
Young Jae Kim
Jae Young Lee
Joon Koo Han
Byung-Ihn Choi
Publication date: 01-09-2014
Publisher: Springer Berlin Heidelberg
Published in: European Radiology / Issue 9/2014
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-014-3260-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Dynamic contrast–enhanced ultrasonographic (DCE-US) assessment of the early response after combined gemcitabine and HIFU with low-power treatment for the mouse xenograft model of human pancreatic cancer

Abstract

Purpose

Materials and methods

Results

Conclusion

Key points

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Materials and methods

Results

Conclusion

Key points

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