Published in:
Open Access
01-12-2009 | Research
Down-regulated miR-9 and miR-433 in human gastric carcinoma
Authors:
Hongchun Luo, Hongbin Zhang, Zhenzhen Zhang, Xia Zhang, Bo Ning, Jinjun Guo, Na Nie, Bo Liu, Xiaoling Wu
Published in:
Journal of Experimental & Clinical Cancer Research
|
Issue 1/2009
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Abstract
Background
MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.
Methods
To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.
Results
We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.
Conclusion
Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.